Construct: ORF TRCN0000476743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006714.1_s317c1
- Derived from:
- ccsbBroadEn_08630
- DNA Barcode:
- TACCCAATACGCTCCCGGGGTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHA11 (56138)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476743
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56138 | PCDHA11 | protocadherin alpha 11 | NM_031861.2 | 99.9% | 100% | 1407A>G |
| 2 | human | 56138 | PCDHA11 | protocadherin alpha 11 | NM_018902.4 | 84.9% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2499
- ORF length:
- 2430
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttggtttt cagagaaggg gattgggcac cccacgacta cagctctggc 121 ttctcctcct cgaattctgg gaggtgggga gcggccagct ccactactcc gtctcggagg 181 aggccaaaca cggcaccttc gtgggccgca tcgcgcagga cctggggctg gagctggcgg 241 agctggtgca gcgcctgttc cgggtggcgt ccaaaacaca tggggacctt ctggaggtaa 301 atctgcagaa tggcattttg tttgtgaatt ctcggatcga ccgcgaggag ctgtgcgggc 361 agagcgcgga gtgcagcatc cacctggagg tgatcgtgga caggccgctg caggttttcc 421 atgtgaacgt ggaggtgaag gacattaacg acaacccgcc ggtgttctcg ctcagagaac 481 aaaagctgct gattgcggaa tctaagcaat cggactcgcg ttttccacta gagggagctt 541 ctgatgctga cattgaagag aatgctctat tgacctacag gctaagtaaa aatgagtatt 601 tttctttaga ttcaccaaca aatggtaagc agattaaaag actgtcactt attttaaaga 661 agtctctgga tagagagaaa actccggaac ttaatttgct gctaacagct acagacgggg 721 gaaaaccaga gcttactggc accgttcggc tgttagtcca agtcttggat gtcaacgaca 781 atgatccaga gtttgataaa tcagaatata aggtgagcct tatggaaaat gctgctaaag 841 aaactcttgt gctcaaacta aacgccacag accgagacga aggagtcaat ggagaggtaa 901 catactcctt aatgtcaatt aagcccaatg gaagacactt atttacacta gatcaaaata 961 atggagaagt gagggtcaat ggaactttag attatgaaga aaacaagttt tataaaattg 1021 aagtacaggc tacagataag gggactcccc caatggcagg tcactgtaca gtctgggtgg 1081 aaatcttgga caccaacgat aactctcctg aagtcgccgt gacttccttg tccctcccag 1141 tacgagagga cgctcagccc agcacggtca ttgccctgat cagcgtgtct gaccgtgact 1201 caggtgtcaa cggacaggtg acctgctcgc tgacgcccca cgttcccttc aagctggtgt 1261 ccaccttcaa gaattactac tcgttggtgc tggacagcgc cctggaccgc gagaacgtgt 1321 gggcctatga actggtggtg actgcgcggg atgggggttc gccttctctg tgggccacgg 1381 ccagggtatc cgtggaggtg gccgacgtga acgacaatgc gcctgcgttc gcacagcccg 1441 agtacaccgt gttcgtgaag gagaacaacc caccgggctg ccacatcttc acagtgtcgg 1501 cgcgggacgc ggacgcgcag gagaacgcgc tggtgtccta ctcgctggtg gagcggcggt 1561 tgggcgaccg cgcgctgtcg agctacgtgt cggtgcacgc ggagagcggc aaggtgtacg 1621 cgctgcagcc gttggaccac gaggagctgg agctgctaca gttccaggtg agcgcgcgcg 1681 atgcgggcgt gccgcctctg agcagcaacg tgacgctgca ggtgttcgtg ctggacgaga 1741 acgacaacgc gccggcactg ctggcgactc aggctggcag cgcgggaggc gcagttaaca 1801 agctagtacc gcggtcggtg ggtgcgggcc acgtggtggc gaaggtgcgc gcagtggatg 1861 cggactcagg ctacaacgcg tggctttcat atgaattgca gccggcggcg ggcggctcgc 1921 gcatcccgtt ccgcgtgggg ctgtacacgg gcgagataag cacaacgcgt gccctggacg 1981 aggcagactc gccgcgccac cgacttctag tactggtgaa ggatcacggt gagccggcgc 2041 tgacggccac ggccaccgtg ctggtgtcgt tggtggagag cggacaggcg ccaaaggcct 2101 cttcccggac ttTGGCGGGC GCCGCGAGCC CAGAGGCTGC GCTGGTGGAT GTCAACGTGT 2161 ACCTGATCAT CGCCATCTGC GTGGTGTCCA GCCTCCTGGT ACTCACGCTG CTGCTGTATA 2221 CGGCGCTGTG GTGGTCGGCA ACGCCCACTG AGGGCGCGTG CGCGCCGGGG AAGCCCACGC 2281 TGGTGTGCTC CCGCGCGGTG GGGAGCTGGT CATACTCGCA GCAGAGGCGG CAGAGGGTGT 2341 GCTCTGAGGA GGGCCCACCT AAGACGGACC TCATGGCCTT CAGCCCCAGT CTTCCTCTAG 2401 GTCTGAATAA AGAGGAGGAA GGGGAAAGAC AGGAGCCAGG GTCAAATCAC CCCGGACAGG 2461 TGAGTTTTCT ACAGATTCCA CCTATCAGGA AGTGTATGTT GCCAACTTTC TTGTACAAAG 2521 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2581 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2641 ACGATACCCA ATACGCTCCC GGGGTTCTAC GCGTTAAGTC gacaatcaac ctctggatta 2701 caaaatttgt gaaagatt