Construct: ORF TRCN0000476753
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008553.1_s317c1
- Derived from:
- ccsbBroadEn_13856
- DNA Barcode:
- CAACAATCCGAAGTTAAAACCTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EPS15 (2060)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476753
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2060 | EPS15 | epidermal growth factor rec... | XM_017000617.2 | 91.9% | 91.5% | (many diffs) |
| 2 | human | 2060 | EPS15 | epidermal growth factor rec... | XM_017000618.2 | 88.3% | 87.9% | (many diffs) |
| 3 | human | 2060 | EPS15 | epidermal growth factor rec... | XM_017000616.2 | 87.9% | 87.6% | (many diffs) |
| 4 | human | 2060 | EPS15 | epidermal growth factor rec... | NM_001981.3 | 84.9% | 84.5% | (many diffs) |
| 5 | human | 2060 | EPS15 | epidermal growth factor rec... | XM_005270618.3 | 81.6% | 81.2% | (many diffs) |
| 6 | human | 2060 | EPS15 | epidermal growth factor rec... | XM_017000615.2 | 81.5% | 81.2% | (many diffs) |
| 7 | human | 2060 | EPS15 | epidermal growth factor rec... | XM_017000619.2 | 63.9% | 63.6% | (many diffs) |
| 8 | human | 2060 | EPS15 | epidermal growth factor rec... | XM_017000620.2 | 58.4% | 58% | (many diffs) |
| 9 | human | 2060 | EPS15 | epidermal growth factor rec... | NM_001159969.2 | 48.7% | 46.3% | (many diffs) |
| 10 | mouse | 13858 | Eps15 | epidermal growth factor rec... | NM_007943.3 | 76% | 76.1% | (many diffs) |
| 11 | mouse | 13858 | Eps15 | epidermal growth factor rec... | XM_006502738.2 | 73.1% | 73.2% | (many diffs) |
| 12 | mouse | 13858 | Eps15 | epidermal growth factor rec... | XM_006502739.3 | 70% | 69.9% | (many diffs) |
| 13 | mouse | 13858 | Eps15 | epidermal growth factor rec... | XM_017319956.1 | 65.1% | 65.1% | (many diffs) |
| 14 | mouse | 13858 | Eps15 | epidermal growth factor rec... | XM_006502740.2 | 62.6% | 62.6% | (many diffs) |
| 15 | mouse | 13858 | Eps15 | epidermal growth factor rec... | NM_001159964.1 | 42.9% | 40.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2343
- ORF length:
- 2277
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcggcggcc cagctctctc tgacacagtt atcaagtggg aatcctgtat 121 atgaaaaata ctatagacag gttgatacag gcaatactgg aagggtgttg gcttctgatg 181 ctgctgcttt cctgaaaaaa tcagggcttc cagacttgat acttggaaag atttgggatt 241 tagccgacac agatggcaaa ggtatcctga acaaacaaga attctttgtt gctttgcgtc 301 ttgtggcatg tgcccagaat ggattggaag tttcactaag tagtttgaac ctggctgttc 361 ctccaccaag atttcatgat accagtagtc ctttgctaat cagtggaacc tctgcagctg 421 agctcccatg ggctgtaaaa cctgaagata aggccaaata tgatgcaata tttgatagtt 481 taagcccagt gaatggattt ctgtctggtg ataaagtgaa accagtgttg ctcaactcta 541 agttacctgt ggatatcctt ggaagagttt gggagttgag tgatattgac catgatggaa 601 tgcttgacag agatgagttt gcagttgcta tgtttttggt atactgtgca ctggagaaag 661 aacctgtgcc aatgtccttg cctccagcct tggtgccacc atctaagaga aaaacgtggg 721 ttgtatcccc tgcagaaaaa gctaaatatg atgaaatctt cctgaaaact gataaagata 781 tggacggatt tgtgtctgga ttggaggtcc gtgaaatatt cttgaaaaca ggtttacctt 841 ctaccttact agcccatata tggtcattat gcgacacaaa ggactgtggg aagctttcaa 901 aggatcagtt tgccttggct tttcacttaa tcagtcagaa gttaatcaag ggcattgatc 961 ctcctcacgt tcttactcct gaaatgattc caccatcaga cagggccagt ttacaaaaga 1021 acatcatagg atcaagtcct gttgcagatt tctctgctat taaggaacta gatactctta 1081 acaatgaaat agttgaccta cagagggaaa agaataatgt ggaacaggac cttaaggaga 1141 aggaagatac tattaaacag aggacaagtg aggttcagga tcttcaagat gaagttcaaa 1201 gggagaatac taatctgcaa aaactacagg cccagaaaca gcaggtacag gaactccttg 1261 atgaactgga tgagcagaaa gcccagctgg aggagcaact caaggaagtc agaaagaaat 1321 gtgctgagga ggcccaactg gcaagaagta gtcctgaact actgccttct ggtgtgactg 1381 atgaaaatga ggtgactaca gctgttactg aaaaagtttg ttctgaactc gacaataata 1441 gacattcaaa agaggaagat ccatttaatg tagactcaag ttcgctgaca ggtccagttg 1501 cagatacaaa cttggatttt ttccagtctg atccttttgt tggcagtgat cctttcaagg 1561 atgatccttt tggaaaaatc gatccatttg gtggtgatcc tttcaaaggt tcagatccat 1621 ttgcatcaga ctgtttcttc aggcaatcta ctgatccttt tgccacttca agcactgacc 1681 ctttcagtgc agccaacaat agcagtatta catcggtaga aacgttgaag cacaatgatc 1741 cttttgctcc tggtggaaca gttgttgcag caagcgattc agccacagac ccctttgctt 1801 ctgtttttgg gaatgaatca tttggaggtg gatttgctga cttcagcaca ttgtcaaagg 1861 tcaacaatga agatcctttt cgttcagcca catcgagctc tgtcagcaac gtagtgatta 1921 caaaaaatgt atttgaggaa acatcggtca aaagtgaaga tgaaccccca gcactgccac 1981 caaagatcgg aactccaaca agacccTGCC CTCTACCACC TGGGAAAAGA TCCATCAACA 2041 AATTGGATTC TCCTGATCCC TTTAAACTGA ATGATCCATT TCAGCCTTTC CCAGGCAACG 2101 ATAGCCCCAA AGAAAAAGAT CCTGAAATGT TTTGTGATCC ATTCACTTCT GCTACTACCA 2161 CTACCAATAA AGAGGCTGAT CCAAGCAATT TTGCCAACTT CAGTGCTTAT CCCTCTGAAG 2221 AAGATATGAT CGAATGGGCC AAGAGGGAAA GTGAGAGAGA GGAAGAGCAG AGGCTTGCCC 2281 GACTAAATCA GCAGGAACAA GAAGACTTAG AACTGGCTAT TGCACTCAGC AAATCTGAGA 2341 TATAGAAGCA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA 2401 CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC 2461 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAAC AATCCGAAGT TAAAACCTCC 2521 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt