Construct: ORF TRCN0000476789
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005179.1_s317c1
- Derived from:
- BRDN0000391324
- DNA Barcode:
- AGCCACTTGTTGCCACTCCAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYNE3 (161176)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476789
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 161176 | SYNE3 | spectrin repeat containing ... | NM_152592.5 | 99.6% | 99.5% | (many diffs) |
| 2 | human | 161176 | SYNE3 | spectrin repeat containing ... | XM_005267377.4 | 99.6% | 99.5% | (many diffs) |
| 3 | human | 161176 | SYNE3 | spectrin repeat containing ... | XM_006720063.4 | 99.6% | 99.5% | (many diffs) |
| 4 | human | 161176 | SYNE3 | spectrin repeat containing ... | NM_001363692.1 | 99.3% | 99.2% | (many diffs) |
| 5 | human | 161176 | SYNE3 | spectrin repeat containing ... | XM_011536515.3 | 98.8% | 98.7% | (many diffs) |
| 6 | human | 161176 | SYNE3 | spectrin repeat containing ... | XM_017021057.1 | 50% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2991
- ORF length:
- 2922
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gactcagcag ccccaggacg actttgacag gagcgtggag gatgcccagg 121 catggatgaa ggctgtgcag gaccagctgc aggtcaatga caacacgcag ggaccccgcg 181 cggccctgga ggccaggctg tgggagaccg agaaaatatg ccagctggag cccgaggggc 241 gtgtgagggt ggacctcgtg ctacggatgg ctgaagccct cttggcatgc tgccctgggg 301 accagaagcc cgggatcctg gcccggctga aggacatcaa ggcccaatgg gaggagacag 361 tcacctacat gactcactgt cacagccgca tcgagtgggt gtggctgcac tggagcgagt 421 acctgctggc ccgagatgag ttctaccgct ggttccagaa gatgatggtc acactggagc 481 cccacatcga gctccagctg ggcctgaagg agaagcagtg gcagctgagc cacgcccagg 541 tgctgctgca caacgtggac aaccaggcgg tgctcctgga ccggctgctg gaggaggcag 601 cctccctgtt caacaggatc ggggacccca gcgtggacga agatgcccag aagagaatga 661 aggctgagta cgatgcagtg aaggccaagg cccagaagcg ggtagatctg ctggagcagg 721 tggcccggga gcatgaggag taccaggcag gtgtggacga gttccaactg tggctgaagg 781 cggtggtgga gaaggtgaat ggctgcctgg ggctgcactg caagctgccc atcacgcagc 841 gcctctccac actgcaggac attgccaaag attttcccag gggcgaggag tctctggaga 901 cgctggagga gcagtctgcg ggtgtcattc ggaacacctc tcctttgggt gcagagaaga 961 tcaccggaga actggaagag atgaggaaag ttctggagaa gctgcgcgcc ctctgggagg 1021 aggaggagga gcggctgcgg ggcctgctcc ggtccagggg agcctgggag cagcagatta 1081 agcagctgga ggctgagctc agtgagttca ggatggtcct gcagaggctg gcccaggagg 1141 gcctgcagcc tgcggcgaaa gcggggaccg aggacgaact ggtggcacac tggagacgct 1201 actcggcaac acgggcggcg ctggcctcgg aggagccccg ggtggaccgg ctgcaagccc 1261 agctgaagga gctcatcgtc ttccctcaca acctgaagcc gctctctgat agtgtcatcg 1321 ctaccatcca ggagtatcag agcctgaagg tgaagagcgc gaggctgcgc aatgccgcgg 1381 cggtggagct gtggcagcat ttccagcggc ctctgcagga tctgcagctg tggaaggccc 1441 tggcccagcg gctcttggag gtcactgcca gcctgccgga cctgccttcc cttcacacct 1501 tcctgcccca gatcgaggca gccctgatgg aaagctcccg cctgaaagag ctgctgacga 1561 tgctgcagct gaagaaagac ctcctgattg gcatctttgg ccaggagaga gccacggcac 1621 tcctggagca ggtggcaggt tccatgaggg acagagacct gctgcataac agcctcctgc 1681 agaggaaaag caaactgcag agcctgctcg ctcagcacaa agactttgga gcagcttttg 1741 agcccctgca gaggaagctc ttggacctcc aggtcagggt ccaagccgag aaggggcttc 1801 agcgggacct tcctggaaaa caggcccagc tctcaaggtt gcaggggctg caggaagagg 1861 ggctggactt gggggcacag atggaggctg caaggcctct ggtccaggag aaccccaacc 1921 accagcacaa aatggaccag ctttcctccg acttccaggc cctgcagagg tctctggagg 1981 accttgtgga caggtgtcgg cagagtgtgc aggagcactg caccttcagc caccagctgc 2041 tggagctgcg gcagtggatc gttgtgacca tgcaaaagct ggaggcacac cggggagagg 2101 cgggcccggg ggatgccgag tcccaagagg ccgagtttga gaggctggtg gcagaattcc 2161 cggagaagga ggcccagctg tccctggtgg aagcgcaggg ctggctggtg atggagaagt 2221 cttctccgga gggtgctgcc gtggtgcagg aggagctcag ggagctggca gagtcgtggc 2281 gggccttgag gctgctggaa gaaagtctgc tgagcctcat cagaaactgg catctgcaga 2341 ggatggaagt ggattcgggg aagaaaatgg ttttcaccaa caacatccca aagtcaggat 2401 ttctcatcaa tcccatggat cctattccca ggcatcgtcg acgcgcaaat ctacaggaag 2461 aagaagggag ccatgaagat ttctcccagc tcctgaggaa ctttgggcag tggttgcagg 2521 tggaaaactc caagctggtt agaatcatcg caatgagaac ttctacagct gaggacttga 2581 ggaccagaaa atcgaagctg caggagctgg aggctcgggt gccagaaggt cagcatctct 2641 ttgagaacct ccttcgtctc gggccagcaa gggggacctc ggatgagctg gaagatctgc 2701 gctaccagtg gatgctgtac aagtccaagc tgaaggactc tggccacctg ctgacacaaa 2761 gttctccagg ggagccgact ggattccaaa agactcggcg gtggcgagga ctgggctccc 2821 tcttccggag ggcgtgctgt gtggcgctcc cactgcagct gcttctgctg ctgttcctcc 2881 tcctgctgtt cctgctccca atcagggaag aggaccgcag ctgcaccctg gccaacaact 2941 tcgcccgctc cttcacgctc atgctgcgct acaatggCCC ACCACCCACC TTGCCAACTT 3001 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 3061 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 3121 CTTGTGGAAA GGACGAAGCC ACTTGTTGCC ACTCCAACCA ACGCGTTAAG TCgacaatca 3181 acctctggat tacaaaattt gtgaaagatt