Construct: ORF TRCN0000476794
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002597.1_s317c1
- Derived from:
- ccsbBroadEn_01714
- DNA Barcode:
- TATTATCTAGGACCGGTGTACCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRPC3 (7222)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476794
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7222 | TRPC3 | transient receptor potentia... | NM_003305.2 | 100% | 100% | |
| 2 | human | 7222 | TRPC3 | transient receptor potentia... | NM_001130698.2 | 92% | 92% | 1_219del |
| 3 | human | 7222 | TRPC3 | transient receptor potentia... | XM_011532218.3 | 91.9% | 91.9% | 1_219del;2544_2545insCAG |
| 4 | human | 7222 | TRPC3 | transient receptor potentia... | XM_011532217.3 | 90.5% | 90.5% | 1_219del;2463_2510del |
| 5 | human | 7222 | TRPC3 | transient receptor potentia... | XM_017008578.2 | 90.3% | 90.3% | 1_219del;2463_2510del;2592_2593insCAG |
| 6 | human | 7222 | TRPC3 | transient receptor potentia... | NM_001366479.2 | 89% | 89% | 1_219del;2462_2463ins84 |
| 7 | mouse | 22065 | Trpc3 | transient receptor potentia... | NM_019510.2 | 81.7% | 89.3% | (many diffs) |
| 8 | mouse | 22065 | Trpc3 | transient receptor potentia... | XM_006535439.3 | 78.7% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2613
- ORF length:
- 2544
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagggaagc ccatccctga gacgcatgac agtgatgcgg gagaagggcc 121 ggcgccaggc tgtcaggggc ccggccttca tgttcaatga ccgcggcacc agcctcaccg 181 ccgaggagga gcgcttcctc gacgccgccg agtacggcaa catcccagtg gtgcgcaaga 241 tgctggagga gtccaagacg ctgaacgtca actgcgtgga ctacatgggc cagaacgcgc 301 tgcagctggc tgtgggcaac gagcacctgg aggtgaccga gctgctgctc aagaaggaga 361 acctggcgcg cattggcgac gccctgctgc tcgccatcag caagggctac gtgcgcatcg 421 tagaggccat cctcaaccac cctggcttcg cggccagcaa gcgtctcact ctgagcccct 481 gtgagcagga gctgcaggac gacgacttct acgcttacga cgaggacggc acgcgcttct 541 cgccggacat cacccccatc atcctggcgg cgcactgcca gaaatacgaa gtggtgcaca 601 tgctgctgat gaagggtgcc aggatcgagc ggccgcacga ctatttctgc aagtgcgggg 661 actgcatgga gaagcagagg cacgactcct tcagccactc acgctcgagg atcaatgcct 721 acaaggggct ggccagcccg gcttacctct cattgtccag cgaggacccg gtgcttacgg 781 ccctagagct cagcaacgag ctggccaagc tggccaacat agagaaggag ttcaagaatg 841 actatcggaa gctctccatg caatgcaaag actttgtagt gggtgtgctg gatctctgcc 901 gagactcaga agaggtagaa gccattctga atggagatct ggaatcagca gagcctctgg 961 aggtacacag gcacaaagct tcattaagtc gtgtcaaact tgccattaag tatgaagtca 1021 aaaagtttgt ggctcatccc aactgccagc agcagctctt gacgatctgg tatgagaacc 1081 tctcaggcct aagggagcag accatagcta tcaagtgtct cgttgtgctg gtcgtggccc 1141 tgggccttcc attcctggcc attggctact ggatcgcacc ttgcagcagg ctggggaaaa 1201 ttctgcgaag cccttttatg aagtttgtag cacatgcagc ttctttcatc atcttcctgg 1261 gtctgcttgt gttcaatgcc tcagacaggt tcgaaggcat caccacgctg cccaatatca 1321 cagttactga ctatcccaaa cagatcttca gggtgaaaac cacccagttt acatggactg 1381 aaatgctaat tatggtctgg gttcttggaa tgatgtggtc tgaatgtaaa gagctctggc 1441 tggaaggacc tagggaatac attttgcagt tgtggaatgt gcttgacttt gggatgctgt 1501 ccatcttcat tgctgctttc acagccagat tcctagcttt ccttcaggca acgaaggcac 1561 aacagtatgt ggacagttac gtccaagaga gtgacctcag tgaagtgaca ctcccaccag 1621 agatacagta tttcacttat gctagagata aatggctccc ttctgaccct cagattatat 1681 ctgaaggcct ttatgccata gctgttgtgc tcagcttctc tcggattgcg tacatcctcc 1741 ctgcaaatga gagctttggc cccctgcaga tctctcttgg aaggactgta aaggacatat 1801 tcaagttcat ggtcctcttt attatggtgt tttttgcctt tatgattggc atgttcatac 1861 tttattctta ctaccttggg gctaaagtta atgctgcttt taccactgta gaagaaagtt 1921 tcaagacttt attttggtca atatttgggt tgtctgaagt gacttccgtt gtgctcaaat 1981 atgatcacaa attcatagaa aatattggat acgttcttta tggaatatac aatgtaacta 2041 tggtggtcgt tttactcaac atgctaattg ctatgattaa tagctcatat caagaaattg 2101 aggatgacag tgatgtagaa tggaagtttg ctcgttcaaa actttggtta tcctattttg 2161 atgatggaaa aacattacct ccacctttca gtctagttcc TAGTCCAAAA TCATTTGTTT 2221 ATTTCATCAT GCGAATTGTT AACTTTCCCA AATGCAGAAG GAGAAGGCTT CAGAAGGATA 2281 TAGAAATGGG AATGGGTAAC TCAAAGTCCA GGTTAAACCT CTTCACTCAG TCTAACTCAA 2341 GAGTTTTTGA ATCACACAGT TTTAACAGCA TTCTCAATCA GCCAACACGT TATCAGCAGA 2401 TAATGAAAAG ACTTATAAAG CGGTATGTTT TGAAAGCACA AGTAGACAAA GAAAATGATG 2461 AAGTTAATGA AGGTGAATTA AAAGAAATCA AGCAAGATAT CTCCAGCCTT CGTTATGAAC 2521 TTTTGGAAGA CAAGAGCCAA GCAACTGAGG AATTAGCCAT TCTAATTCAT AAACTTAGTG 2581 AGAAACTGAA TCCCAGCATG CTGAGATGTG AATTGCCAAC TTTCTTGTAC AAAGTGGTTG 2641 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2701 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 2761 TTATCTAGGA CCGGTGTACC TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2821 ttgtgaaaga tt