Construct: ORF TRCN0000476798
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000613.1_s317c1
- Derived from:
- ccsbBroadEn_00964
- DNA Barcode:
- TCAGATTCTGTTACAGAAGTAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMAD7 (4092)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476798
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4092 | SMAD7 | SMAD family member 7 | NM_005904.3 | 100% | 100% | |
| 2 | human | 4092 | SMAD7 | SMAD family member 7 | NM_001190821.1 | 99.7% | 99.7% | 666_667insGCA |
| 3 | human | 4092 | SMAD7 | SMAD family member 7 | NM_001190823.1 | 53.7% | 48.2% | (many diffs) |
| 4 | human | 4092 | SMAD7 | SMAD family member 7 | NM_001190822.2 | 49.5% | 49.5% | 0_1ins645 |
| 5 | mouse | 17131 | Smad7 | SMAD family member 7 | NM_001042660.1 | 95.3% | 98.1% | (many diffs) |
| 6 | mouse | 17131 | Smad7 | SMAD family member 7 | XM_006525703.2 | 95.1% | 97.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1347
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttcaggacc aaacgatctg cgctcgtccg gcgtctctgg aggagccgtg 121 cgcccggcgg cgaggacgag gaggagggcg cagggggagg tggaggagga ggcgagctgc 181 ggggagaagg ggcgacggac agccgagcgc atggggccgg tggcggcggc ccgggcaggg 241 ctggatgctg cctgggcaag gcggtgcgag gtgccaaagg tcaccaccat ccccacccgc 301 cagccgcggg cgccggcgcg gccgggggcg ccgaggcgga tctgaaggcg ctcacgcact 361 cggtgctcaa gaaactgaag gagcggcagc tggagctgct gctccaggcc gtggagtccc 421 gcggcgggac gcgcaccgcg tgcctcctgc tgcccggccg cctggactgc aggctgggcc 481 cgggggcgcc cgccggcgcg cagcctgcgc agccgccctc gtcctactcg ctccccctcc 541 tgctgtgcaa agtgttcagg tggccggatc tcaggcattc ctcggaagtc aagaggctgt 601 gttgctgtga atcttacggg aagatcaacc ccgagctggt gtgctgcaac ccccatcacc 661 ttagccgact ctgcgaacta gagtctcccc cccctcctta ctccagatac ccgatggatt 721 ttctcaaacc aactgcagac tgtccagatg ctgtgccttc ctccgctgaa acagggggaa 781 cgaattatct ggcccctggg gggctttcag attcccaact tcttctggag cctggggatc 841 ggtcacactg gtgcgtggtg gcatactggg aggagaagac gagagtgggg aggctctact 901 gtgtccagga gccctctctg gatatcttct atgatctacc tcaggggaat ggcttttgcc 961 tcggacagct caattcggac aacaagagtc agctggtgca gaaggtgcgg agcaaaatcg 1021 gctgcggcat ccagcTGACG CGGGAGGTGG ATGGTGTGTG GGTGTACAAC CGCAGCAGTT 1081 ACCCCATCTT CATCAAGTCC GCCACACTGG ACAACCCGGA CTCCAGGACG CTGTTGGTAC 1141 ACAAGGTGTT CCCCGGTTTC TCCATCAAGG CTTTCGACTA CGAGAAGGCG TACAGCCTGC 1201 AGCGGCCCAA TGACCACGAG TTTATGCAGC AGCCGTGGAC GGGCTTTACC GTGCAGATCA 1261 GCTTTGTGAA GGGCTGGGGC CAGTGCTACA CCCGCCAGTT CATCAGCAGC TGCCCGTGCT 1321 GGCTAGAGGT CATCTTCAAC AGCCGGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1381 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1441 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCAGATTC 1501 TGTTACAGAA GTAAAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1561 aagatt