Construct: ORF TRCN0000476821

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002662.1_s317c1
Derived from:
ccsbBroadEn_02951
DNA Barcode:
GCACAATATTAATATTCAAATAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IL1RAPL2 (26280)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476821
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26280 IL1RAPL2 interleukin 1 receptor acce... NM_017416.2 100% 100%
2 human 26280 IL1RAPL2 interleukin 1 receptor acce... XM_011530905.2 81.9% 81.9% 0_1ins372
3 mouse 60367 Il1rapl2 interleukin 1 receptor acce... NM_030688.2 91.7% 94.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2127
ORF length:
2058
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaagccacca tttcttttgg cccttgtggt ctgttctgta gtcagcacaa
121atctgaagat ggtgtcaaag agaaattctg tggatggctg cattgactgg tcagtggatc
181tcaagacata catggctttg gcaggtgaac cagtccgagt gaaatgtgcc cttttctaca
241gttatattcg taccaactat agcacggccc agagcactgg gctcaggctt atgtggtaca
301aaaacaaagg tgatttggaa gagcccatca tcttttcaga ggtcaggatg agcaaagagg
361aagattcaat atggtttcac tcagctgagg cacaagacag tggattctac acttgtgttt
421taagaaactc aacatattgc atgaaggtgt caatgtcctt gactgttgca gagaatgaat
481caggcctgtg ctacaacagc aggatccgct atttagaaaa atctgaagtc actaaaagaa
541aggagatctc ctgtccagac atggatgact ttaaaaagtc cgatcaggag cctgatgttg
601tgtggtataa ggaatgcaag ccaaaaatgt ggagaagcat aataatacag aaaggaaatg
661ctcttctgat ccaagaagtt caagaagaag atggaggaaa ttacacatgt gaacttaaat
721atgaaggaaa acttgtaaga cgaacaactg aattgaaagt tacagcttta ctcacagaca
781agcctcccaa gccattgttc cccatggaga atcagccaag tgttatagat gtccagctgg
841gtaagcctct gaacatcccc tgcaaagcat tcttcggatt cagtggagag tctgggccaa
901tgatctactg gatgaaagga gaaaagttta ttgaagaact ggcaggtcac attagagaag
961gtgaaataag gcttctcaaa gagcatcttg gagaaaaaga agttgaattg gcactcatct
1021ttgactcagt tgtggaagct gacctggcga attatacctg ccatgttgaa aaccgaaatg
1081gacggaaaca tgccagtgtt ttgctgcgta aaaaggattt aatctataaa attgagcttg
1141cagggggcct gggagcaatc ttcctcctcc ttgtactgct ggtggtcatt tacaaatgct
1201acaacattga attgatgctc ttctacaggc agcactttgg agctgatgaa actaatgatg
1261acaacaagga atatgatgcc tatctctctt acacaaaagt ggaccaagat actttagact
1321gtgacaatcc tgaagaagag cagtttgctc ttgaagtact gccagatgtc ctggaaaaac
1381actatggata taaactcttc atcccagaaa gagacctgat tccaagtgga acatacatgg
1441aagatctcac aagatatgtt gaacaaagca gaagacttat tatcgtgcta actccagact
1501atattctcag acggggatgg agtattttcg aactggaaag cagactccat aacatgctag
1561tcagtggaga aatcaaagtg attttgattg agtgtacaga attaaaaggg aaagtgaatt
1621gccaggaagt ggaatcacta aagcgtagca tcaaacttct gtccctgatc aagtggaagg
1681gatccaaaag cagcaaatta aattctaagt tttggaagca cttagtatat gaaatgccca
1741tcaagaaaaa agaaatgcta ccTCGGTGCC ATGTTCTGGA CTCCGCAGAA CAAGGACTTT
1801TTGGAGAACT CCAGCCTATA CCCTCTATTG CCATGACCAG TACTTCAGCC ACTCTGGTGT
1861CATCTCAGGC TGATCTCCCT GAATTCCACC CTTCAGATTC AATGCAAATC AGGCACTGTT
1921GCAGAGGTTA TAAACATGAG ATACCAGCCA CGACCTTGCC AGTACCTTCC TTAGGCAACC
1981ACCATACTTA TTGTAACCTG CCTCTGACGC TACTCAACGG ACAGCTACCC CTTAATAACA
2041CCCTGAAAGA TACCCAGGAA TTTCACAGGA ACAGTTCTTT GCTGCCTTTA TCCTCCAAAG
2101AGCTTAGCTT TACCAGTGAT ATTTGGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
2161GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2221AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCACAATA
2281TTAATATTCA AATAATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2341aagatt

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