Construct: ORF TRCN0000476821
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002662.1_s317c1
- Derived from:
- ccsbBroadEn_02951
- DNA Barcode:
- GCACAATATTAATATTCAAATAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL1RAPL2 (26280)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476821
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26280 | IL1RAPL2 | interleukin 1 receptor acce... | NM_017416.2 | 100% | 100% | |
| 2 | human | 26280 | IL1RAPL2 | interleukin 1 receptor acce... | XM_011530905.2 | 81.9% | 81.9% | 0_1ins372 |
| 3 | mouse | 60367 | Il1rapl2 | interleukin 1 receptor acce... | NM_030688.2 | 91.7% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2127
- ORF length:
- 2058
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagccacca tttcttttgg cccttgtggt ctgttctgta gtcagcacaa 121 atctgaagat ggtgtcaaag agaaattctg tggatggctg cattgactgg tcagtggatc 181 tcaagacata catggctttg gcaggtgaac cagtccgagt gaaatgtgcc cttttctaca 241 gttatattcg taccaactat agcacggccc agagcactgg gctcaggctt atgtggtaca 301 aaaacaaagg tgatttggaa gagcccatca tcttttcaga ggtcaggatg agcaaagagg 361 aagattcaat atggtttcac tcagctgagg cacaagacag tggattctac acttgtgttt 421 taagaaactc aacatattgc atgaaggtgt caatgtcctt gactgttgca gagaatgaat 481 caggcctgtg ctacaacagc aggatccgct atttagaaaa atctgaagtc actaaaagaa 541 aggagatctc ctgtccagac atggatgact ttaaaaagtc cgatcaggag cctgatgttg 601 tgtggtataa ggaatgcaag ccaaaaatgt ggagaagcat aataatacag aaaggaaatg 661 ctcttctgat ccaagaagtt caagaagaag atggaggaaa ttacacatgt gaacttaaat 721 atgaaggaaa acttgtaaga cgaacaactg aattgaaagt tacagcttta ctcacagaca 781 agcctcccaa gccattgttc cccatggaga atcagccaag tgttatagat gtccagctgg 841 gtaagcctct gaacatcccc tgcaaagcat tcttcggatt cagtggagag tctgggccaa 901 tgatctactg gatgaaagga gaaaagttta ttgaagaact ggcaggtcac attagagaag 961 gtgaaataag gcttctcaaa gagcatcttg gagaaaaaga agttgaattg gcactcatct 1021 ttgactcagt tgtggaagct gacctggcga attatacctg ccatgttgaa aaccgaaatg 1081 gacggaaaca tgccagtgtt ttgctgcgta aaaaggattt aatctataaa attgagcttg 1141 cagggggcct gggagcaatc ttcctcctcc ttgtactgct ggtggtcatt tacaaatgct 1201 acaacattga attgatgctc ttctacaggc agcactttgg agctgatgaa actaatgatg 1261 acaacaagga atatgatgcc tatctctctt acacaaaagt ggaccaagat actttagact 1321 gtgacaatcc tgaagaagag cagtttgctc ttgaagtact gccagatgtc ctggaaaaac 1381 actatggata taaactcttc atcccagaaa gagacctgat tccaagtgga acatacatgg 1441 aagatctcac aagatatgtt gaacaaagca gaagacttat tatcgtgcta actccagact 1501 atattctcag acggggatgg agtattttcg aactggaaag cagactccat aacatgctag 1561 tcagtggaga aatcaaagtg attttgattg agtgtacaga attaaaaggg aaagtgaatt 1621 gccaggaagt ggaatcacta aagcgtagca tcaaacttct gtccctgatc aagtggaagg 1681 gatccaaaag cagcaaatta aattctaagt tttggaagca cttagtatat gaaatgccca 1741 tcaagaaaaa agaaatgcta ccTCGGTGCC ATGTTCTGGA CTCCGCAGAA CAAGGACTTT 1801 TTGGAGAACT CCAGCCTATA CCCTCTATTG CCATGACCAG TACTTCAGCC ACTCTGGTGT 1861 CATCTCAGGC TGATCTCCCT GAATTCCACC CTTCAGATTC AATGCAAATC AGGCACTGTT 1921 GCAGAGGTTA TAAACATGAG ATACCAGCCA CGACCTTGCC AGTACCTTCC TTAGGCAACC 1981 ACCATACTTA TTGTAACCTG CCTCTGACGC TACTCAACGG ACAGCTACCC CTTAATAACA 2041 CCCTGAAAGA TACCCAGGAA TTTCACAGGA ACAGTTCTTT GCTGCCTTTA TCCTCCAAAG 2101 AGCTTAGCTT TACCAGTGAT ATTTGGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2161 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2221 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCACAATA 2281 TTAATATTCA AATAATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2341 aagatt