Construct: ORF TRCN0000476823
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018413.1_s317c1
- Derived from:
- ccsbBroadEn_12735
- DNA Barcode:
- GGTCTGTTTCACTGTGTGTTTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCF7L1 (83439)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476823
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83439 | TCF7L1 | transcription factor 7 like 1 | NM_031283.3 | 99.7% | 99.6% | 22_24delGGC;1597G>A |
| 2 | human | 83439 | TCF7L1 | transcription factor 7 like 1 | XM_006712109.2 | 99.6% | 99.4% | 22_24delGGC;656_658delCAG;1600G>A |
| 3 | mouse | 21415 | Tcf7l1 | transcription factor 7 like... | NM_009332.3 | 89.8% | 95.9% | (many diffs) |
| 4 | mouse | 21415 | Tcf7l1 | transcription factor 7 like... | NM_001079822.2 | 87.7% | 93.6% | (many diffs) |
| 5 | mouse | 21415 | Tcf7l1 | transcription factor 7 like... | XM_006505882.1 | 66.2% | 71% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1830
- ORF length:
- 1761
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccccagctc ggcggcgggg gcggcggcgg cggcggcagc gggggaggcg 121 gcggctccag cgccggggcg gccggcggag gggacgacct cggggcgaac gacgagctga 181 tccccttcca ggacgagggg ggcgaggagc aggagccgag cagcgatagc gcctcggcgc 241 agcgggacct agacgaggtc aagtcgtccc tggtcaacga gtcggagaac cagagcagca 301 gctcggactc ggaggcggag aggcgcccgc agcccgtccg ggacactttc cagaagccgc 361 gggactattt cgccgaagtg agaaggcctc aggacagcgc gttctttaaa ggacccccgt 421 accctgggta ccccttcctg atgatcccgg acctgagcag cccgtacctc tccaacggac 481 ccctgtctcc cggaggagcg cgcacctacc tgcagatgaa atggcccctc ctcgatgtcc 541 cctccagcgc cacagtcaag gacacgaggt caccatctcc agcacacttg tctaataaag 601 ttcctgtcgt tcagcacccg catcacatgc atccgctgac tcccctcatc acctacagca 661 atgaccactt ctcccccggc tcccctccca cccacctctc cccagagatc gatccaaaga 721 caggaatccc ccggccccct cacccatccg agctgtcacc gtattaccca ctctctcccg 781 gagctgtcgg acaaatcccc caccccctcg gctggctcgt cccacagcaa ggccagccca 841 tgtactccct tcctcccggt ggcttccggc acccttaccc cgccctcgcc atgaacgcct 901 cgatgtccag cctggtctcc agtcggttct ctcctcacat ggtggctcct gcccaccctg 961 gcctgcccac ctcagggatc ccccaccctg ccatcgtctc ccccatcgtc aagcaggaac 1021 cggcaccccc cagcctgagc cctgcagtga gcgtgaaatc accagtcacc gtgaaaaagg 1081 aggaggaaaa gaagccccac gtgaagaagc ctctgaatgc cttcatgttg tatatgaagg 1141 agatgagggc caaggtggtg gctgagtgca ccctgaagga aagtgcagcc attaaccaga 1201 tccttggaag aaagtggcac aacctgtctc gagaagaaca ggccaagtac tacgagctgg 1261 cccggaagga gcggcagctt cactcgcagc tctacccaac ctggtcagcc cgggacaact 1321 atggtaagaa aaagaagagg aagagagaaa agcagctgtc ccagacacag tcacagcagc 1381 aagtccagga ggcagagggt gccctggcct ccaagagcaa gaagccatgt gttcagtacc 1441 tgccccccga gaagccctgt gacagccctg cctcctccca cgggagcatg ctggactccc 1501 cggccactcc ctctgcagct ttggcctcac cagctgcccc tgctgccacc cattcggagc 1561 aagcccagcc cctctccctc accaccaaac cagaaacccg ggcccagctg gctctccacT 1621 CTGCCGCCTT CCTGTCGGCT AAGGCTGCAG CCTCCTCCTC TAGGCAGATG GGCAGCCAGC 1681 CTCCCCTCCT GTCCCGGCCC CTCCCCCTTG GGTCCATGCC CACAGCTCTG CTGGCCTCTC 1741 CCCCGTCCTT CCCCGCCACG CTCCATGCCC ACCAGGCCCT CCCGGTGCTA CAGGCCCAGC 1801 CTCTTTCCCT GGTCACCAAG TCTGCCCACT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1861 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1921 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGTCT 1981 GTTTCACTGT GTGTTTTTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2041 tgaaagatt