Construct: ORF TRCN0000476830
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007639.1_s317c1
- Derived from:
- ccsbBroadEn_03648
- DNA Barcode:
- ATAGCAGCTAGTCCCGCTTTCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEPTIN11 (55752)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476830
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55752 | SEPTIN11 | septin 11 | NM_018243.4 | 100% | 100% | |
| 2 | human | 55752 | SEPTIN11 | septin 11 | XM_017008404.1 | 98.9% | 99% | 1275_1275delCinsTGCAAGCTTCACA |
| 3 | human | 55752 | SEPTIN11 | septin 11 | XM_017008403.1 | 98.8% | 99.3% | 1274_1275insTGCAAG;1279_1287delTTCTTTATGinsACA |
| 4 | human | 55752 | SEPTIN11 | septin 11 | NM_001306147.2 | 96.2% | 94.1% | (many diffs) |
| 5 | human | 55752 | SEPTIN11 | septin 11 | XM_017008400.2 | 96.2% | 94.1% | (many diffs) |
| 6 | human | 55752 | SEPTIN11 | septin 11 | XM_017008402.1 | 95.2% | 93.2% | (many diffs) |
| 7 | human | 55752 | SEPTIN11 | septin 11 | XM_017008401.1 | 95.1% | 93.5% | (many diffs) |
| 8 | human | 55752 | SEPTIN11 | septin 11 | XM_006714259.4 | 86.7% | 86.7% | 0_1ins171 |
| 9 | mouse | 52398 | Sept11 | septin 11 | NM_001009818.2 | 88.9% | 99.5% | (many diffs) |
| 10 | mouse | 52398 | Sept11 | septin 11 | NM_001310669.1 | 88.2% | 98.3% | (many diffs) |
| 11 | mouse | 52398 | Sept11 | septin 11 | NM_001310671.1 | 88% | 98.8% | (many diffs) |
| 12 | mouse | 52398 | Sept11 | septin 11 | XM_017320981.1 | 87.9% | 99% | (many diffs) |
| 13 | mouse | 52398 | Sept11 | septin 11 | NM_001347377.1 | 87.8% | 98.1% | (many diffs) |
| 14 | mouse | 52398 | Sept11 | septin 11 | XM_006535104.3 | 87.8% | 98.1% | (many diffs) |
| 15 | mouse | 52398 | Sept11 | septin 11 | XM_017320980.1 | 87.8% | 98.1% | (many diffs) |
| 16 | mouse | 52398 | Sept11 | septin 11 | XM_011249532.2 | 86.5% | 94.3% | (many diffs) |
| 17 | mouse | 52398 | Sept11 | septin 11 | XM_006535106.3 | 86.3% | 95.8% | (many diffs) |
| 18 | mouse | 52398 | Sept11 | septin 11 | XM_006535100.3 | 85.8% | 93.2% | (many diffs) |
| 19 | mouse | 52398 | Sept11 | septin 11 | XM_006535101.3 | 85.8% | 93.2% | (many diffs) |
| 20 | mouse | 52398 | Sept11 | septin 11 | XM_011249531.2 | 85.8% | 93.2% | (many diffs) |
| 21 | mouse | 52398 | Sept11 | septin 11 | XM_006535103.3 | 85.6% | 93.6% | (many diffs) |
| 22 | mouse | 52398 | Sept11 | septin 11 | XM_006535102.3 | 85.5% | 93.9% | (many diffs) |
| 23 | mouse | 52398 | Sept11 | septin 11 | XM_017320978.1 | 85.5% | 93.9% | (many diffs) |
| 24 | mouse | 52398 | Sept11 | septin 11 | XM_006535098.3 | 85.4% | 93% | (many diffs) |
| 25 | mouse | 52398 | Sept11 | septin 11 | XM_006535099.3 | 85.4% | 93% | (many diffs) |
| 26 | mouse | 52398 | Sept11 | septin 11 | XM_011249530.2 | 85.4% | 93% | (many diffs) |
| 27 | mouse | 52398 | Sept11 | septin 11 | XM_006535108.3 | 76.2% | 84.9% | (many diffs) |
| 28 | mouse | 52398 | Sept11 | septin 11 | XM_017320982.1 | 76.2% | 84.9% | (many diffs) |
| 29 | mouse | 52398 | Sept11 | septin 11 | XR_389284.3 | 28.1% | (many diffs) | |
| 30 | mouse | 52398 | Sept11 | septin 11 | XR_001784719.1 | 11.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1353
- ORF length:
- 1287
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgtggccgtg gggagaccgt ctaatgaaga gcttcgaaac ttgtctttgt 121 ctggccatgt gggatttgac agcctccctg accagctggt caacaagtct acttctcaag 181 gattctgttt caacatcctt tgtgttggtg agacaggcat tggcaaatcc acgttaatgg 241 acactttgtt caacaccaaa tttgaaagtg acccagctac tcacaatgaa ccaggtgttc 301 ggttaaaagc cagaagttat gagcttcagg aaagcaatgt acggctgaag ttaaccattg 361 ttgacaccgt gggatttgga gaccagataa ataaagatga cagctataag ccgatagtag 421 aatatattga tgcccagttc gaggcctacc tgcaagagga attgaagatt aaacgttctc 481 tcttcaacta ccatgacacg aggatccatg cctgcctcta ctttattgcc cctactggac 541 attcactaaa gtccctggat ctggtcacca tgaaaaagct ggacagtaag gtgaacatca 601 ttccaataat tgcaaaagct gacaccattg ccaagaatga actgcacaaa ttcaagagta 661 agatcatgag tgaactggtc agcaatgggg tccagatata tcagtttccc actgatgaag 721 aaacggtggc agagattaac gcaacaatga gtgtccatct cccatttgca gtggttggca 781 gcaccgaaga ggtgaagatt ggcaacaaga tggcaaaggc caggcagtac ccctggggtg 841 tggtgcaggt tgagaatgaa aatcattgcg attttgtgaa acttcgagag atgctgatcc 901 gcgtgaacat ggaggacttg cgagagcaga ctcacacccg ccactatgaa ttGTACCGAC 961 GCTGTAAGCT TGAAGAGATG GGGTTCAAGG ACACTGACCC TGACAGCAAA CCCTTCAGTC 1021 TTCAGGAGAC ATATGAAGCA AAAAGGAATG AATTCCTGGG AGAACTGCAG AAGAAAGAAG 1081 AAGAAATGAG ACAAATGTTT GTTATGAGAG TGAAGGAGAA AGAAGCTGAA CTTAAGGAGG 1141 CAGAGAAAGA GCTTCACGAG AAGTTTGACC TTCTAAAGCG GACACACCAA GAAGAAAAGA 1201 AGAAAGTGGA AGACAAGAAG AAGGAGCTTG AGGAGGAGGT GAACAACTTC CAGAAGAAGA 1261 AAGCAGCGGC TCAGTTACTA CAGTCCCAGG CCCAGCAATC TGGGGCCCAG CAAACCAAGA 1321 AAGACAAGGA TAAGAAAAAT GCAAGCTTCA CATACCCAAC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 1501 AGCAGCTAGT CCCGCTTTCC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt