Construct: ORF TRCN0000476863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004373.1_s317c1
- Derived from:
- ccsbBroadEn_11676
- DNA Barcode:
- ATCCATATACTTACAGTTGACGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D2B (23102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476863
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | NM_015079.6 | 86.2% | 86.2% | 1_378del |
| 2 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | NM_144572.1 | 81.8% | 80.5% | 1_378del;2695_2746del;2795_2889del |
| 3 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XM_017022022.1 | 81.7% | 80.2% | 0_1ins312;2005_2056del;2105_2199del |
| 4 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XM_011521387.2 | 77.9% | 76.7% | (many diffs) |
| 5 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XR_001751162.1 | 71.9% | (many diffs) | |
| 6 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XR_001751163.1 | 36.6% | (many diffs) | |
| 7 | mouse | 67016 | Tbc1d2b | TBC1 domain family, member 2B | XM_011242794.2 | 81.7% | 82.3% | (many diffs) |
| 8 | mouse | 67016 | Tbc1d2b | TBC1 domain family, member 2B | NM_194334.2 | 71.1% | 71.6% | (many diffs) |
| 9 | mouse | 67016 | Tbc1d2b | TBC1 domain family, member 2B | XR_001778980.1 | 33.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2433
- ORF length:
- 2364
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacttactgg ttacaggagc ttcagcagaa gagatgggaa tattgtaaca 121 gtcttgacat ggtcaagtgg gacagcagga cctctccaac tcccggggat tttcctaagg 181 gtcttgtagc cagagataac actgatttaa tttacccaca cccaaatgct tctgcagaaa 241 aagccagaaa tgtcctagct gtggagactg tgcctggaga gctggtggga gaacaagctg 301 caaatcagcc cgccccaggg catccaaatt ccattaattt ttactctttg aaacagtggg 361 gcaatgagct caagaattcg atgtcttctt tccgtcctgg gagaggacat aatgatagtc 421 ggaggactgt gttttatacc aatgaagagt gggaactttt agacccaacc cctaaggacc 481 tagaggagtc catagtacag gaagaaaaga agaagctgac ccctgaagga aacaaaggag 541 taactggctc aggattcccc tttgattttg gacgtaaccc ctacaaagga aagcgccctt 601 tgaaagacat aattgggtcg tacaaaaatc gtcacagcag tggtgaccct tcaagtgaag 661 gcacatcagg cagtggcagc gtcagcatca ggaagccggc ctccgaaatg caactgcagg 721 tccagagcca gcaggaagag ctggaacagt taaagaaaga cctgtccagt cagaaggagc 781 ttgttcgact gctccagcag acagtccggt catcccagta tgacaagtat ttcacaagca 841 gccggctctg tgagggggtc ccaaaggaca cgctcgagct tctgcaccaa aaggatgatc 901 agattctggg ccttaccagc cagctggaga ggttcagctt ggagaaggag agtcttcagc 961 aggaagtaag gacgctgaag agcaaagtgg gcgagctcaa cgagcagctg ggaatgctca 1021 tggagaccat ccaagccaag gacgaggtca tcatcaagct cagcgagggc gagggcaacg 1081 ggcctcctcc caccgtggcg cccagctccc cttcggttgt gcctgttgcc agggaccagc 1141 tggaactgga caggctgaaa gataatctac aggggtacaa aacccaaaac aaatttctaa 1201 ataaggagat tttggaactc tcagctctac gaagaaatgc agaaaggaga gagagggatc 1261 tgatggcaaa gtattctagc ctggaagcca agctctgcca gatagaaagt aaatacctga 1321 tattgctcca agaaatgaag acaccagtgt gctcagaaga ccaggggccc acccgggagg 1381 tcatagccca gttgctggag gatgctctgc aggttgagag ccaagagcag ccggagcaag 1441 catttgttaa acctcatctt gtcagtgaat atgatattta tgggttcagg actgtacctg 1501 aggatgatga ggaagagaaa ttggttgcca aggtccgcgc gttggatctg aagactctct 1561 acctcacaga aaaccaggaa gtctccactg gggtcaagtg ggaaaactat tttgcaagta 1621 cagtgaacag ggagatgatg tgctctccag agttaaaaaa cctcatccgt gcgggcattc 1681 cccacgagca ccgttccaag gtgtggaagt ggtgtgtgga ccgtcacacc aggaagttca 1741 aggacaacac tgagcctggc cacttccaga ccttgctgca gaaggcgctg gagaaacaga 1801 acccagcctc caagcagatt gagctggact tgctgcgaac tctgcccaac aacaaacatt 1861 actcctgccc cacctcagaa ggcatacaga agttacgcaa tgtcctcctc gccttctcct 1921 ggcggaatcc agatatcggc tactgtcaag gcctaaacag gttggtggca gtggccctcc 1981 tgtacctgga acaagaagat gctttctggt gtctcgttac catagtggaa gttttcatgc 2041 ctcgagacta ttatacaaag actcttttag gaTCCCAGGT GGACCAGCGG GTGTTCAGAG 2101 ACCTTATGAG TGAGAAGCTG CCTCGGTTGC ATGGCCACTT TGAACAGTAC AAAGTCGACT 2161 ACACTCTCAT CACTTTCAAC TGGTTTCTGG TGGTATTTGT GGATAGTGTC GTTAGTGACA 2221 TCCTCTTTAA AATATGGGAC TCTTTCCTTT ATGAAGGACC AAAGGTTATT TTCCGTTTTG 2281 CTCTGGCACT TTTTAAGTAC AAGGAAGAGG AGATTTTGAA ATTGCAAGAT TCGATGTCTA 2341 TATTTAAGTA TCTCCGCTAC TTCACTCGCA CTATCCTTGA TGCTAGATCC GGAACCGACG 2401 CGCCTACCAC TTGGAGAAAG TCCGGCTGGA GCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2461 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2521 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 2581 CCATATACTT ACAGTTGACG CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2641 ttgtgaaaga tt