Construct: ORF TRCN0000476863

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004373.1_s317c1
Derived from:
ccsbBroadEn_11676
DNA Barcode:
ATCCATATACTTACAGTTGACGCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TBC1D2B (23102)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476863
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23102 TBC1D2B TBC1 domain family member 2B NM_015079.6 86.2% 86.2% 1_378del
2 human 23102 TBC1D2B TBC1 domain family member 2B NM_144572.1 81.8% 80.5% 1_378del;2695_2746del;2795_2889del
3 human 23102 TBC1D2B TBC1 domain family member 2B XM_017022022.1 81.7% 80.2% 0_1ins312;2005_2056del;2105_2199del
4 human 23102 TBC1D2B TBC1 domain family member 2B XM_011521387.2 77.9% 76.7% (many diffs)
5 human 23102 TBC1D2B TBC1 domain family member 2B XR_001751162.1 71.9% (many diffs)
6 human 23102 TBC1D2B TBC1 domain family member 2B XR_001751163.1 36.6% (many diffs)
7 mouse 67016 Tbc1d2b TBC1 domain family, member 2B XM_011242794.2 81.7% 82.3% (many diffs)
8 mouse 67016 Tbc1d2b TBC1 domain family, member 2B NM_194334.2 71.1% 71.6% (many diffs)
9 mouse 67016 Tbc1d2b TBC1 domain family, member 2B XR_001778980.1 33.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2433
ORF length:
2364
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gacttactgg ttacaggagc ttcagcagaa gagatgggaa tattgtaaca
121gtcttgacat ggtcaagtgg gacagcagga cctctccaac tcccggggat tttcctaagg
181gtcttgtagc cagagataac actgatttaa tttacccaca cccaaatgct tctgcagaaa
241aagccagaaa tgtcctagct gtggagactg tgcctggaga gctggtggga gaacaagctg
301caaatcagcc cgccccaggg catccaaatt ccattaattt ttactctttg aaacagtggg
361gcaatgagct caagaattcg atgtcttctt tccgtcctgg gagaggacat aatgatagtc
421ggaggactgt gttttatacc aatgaagagt gggaactttt agacccaacc cctaaggacc
481tagaggagtc catagtacag gaagaaaaga agaagctgac ccctgaagga aacaaaggag
541taactggctc aggattcccc tttgattttg gacgtaaccc ctacaaagga aagcgccctt
601tgaaagacat aattgggtcg tacaaaaatc gtcacagcag tggtgaccct tcaagtgaag
661gcacatcagg cagtggcagc gtcagcatca ggaagccggc ctccgaaatg caactgcagg
721tccagagcca gcaggaagag ctggaacagt taaagaaaga cctgtccagt cagaaggagc
781ttgttcgact gctccagcag acagtccggt catcccagta tgacaagtat ttcacaagca
841gccggctctg tgagggggtc ccaaaggaca cgctcgagct tctgcaccaa aaggatgatc
901agattctggg ccttaccagc cagctggaga ggttcagctt ggagaaggag agtcttcagc
961aggaagtaag gacgctgaag agcaaagtgg gcgagctcaa cgagcagctg ggaatgctca
1021tggagaccat ccaagccaag gacgaggtca tcatcaagct cagcgagggc gagggcaacg
1081ggcctcctcc caccgtggcg cccagctccc cttcggttgt gcctgttgcc agggaccagc
1141tggaactgga caggctgaaa gataatctac aggggtacaa aacccaaaac aaatttctaa
1201ataaggagat tttggaactc tcagctctac gaagaaatgc agaaaggaga gagagggatc
1261tgatggcaaa gtattctagc ctggaagcca agctctgcca gatagaaagt aaatacctga
1321tattgctcca agaaatgaag acaccagtgt gctcagaaga ccaggggccc acccgggagg
1381tcatagccca gttgctggag gatgctctgc aggttgagag ccaagagcag ccggagcaag
1441catttgttaa acctcatctt gtcagtgaat atgatattta tgggttcagg actgtacctg
1501aggatgatga ggaagagaaa ttggttgcca aggtccgcgc gttggatctg aagactctct
1561acctcacaga aaaccaggaa gtctccactg gggtcaagtg ggaaaactat tttgcaagta
1621cagtgaacag ggagatgatg tgctctccag agttaaaaaa cctcatccgt gcgggcattc
1681cccacgagca ccgttccaag gtgtggaagt ggtgtgtgga ccgtcacacc aggaagttca
1741aggacaacac tgagcctggc cacttccaga ccttgctgca gaaggcgctg gagaaacaga
1801acccagcctc caagcagatt gagctggact tgctgcgaac tctgcccaac aacaaacatt
1861actcctgccc cacctcagaa ggcatacaga agttacgcaa tgtcctcctc gccttctcct
1921ggcggaatcc agatatcggc tactgtcaag gcctaaacag gttggtggca gtggccctcc
1981tgtacctgga acaagaagat gctttctggt gtctcgttac catagtggaa gttttcatgc
2041ctcgagacta ttatacaaag actcttttag gaTCCCAGGT GGACCAGCGG GTGTTCAGAG
2101ACCTTATGAG TGAGAAGCTG CCTCGGTTGC ATGGCCACTT TGAACAGTAC AAAGTCGACT
2161ACACTCTCAT CACTTTCAAC TGGTTTCTGG TGGTATTTGT GGATAGTGTC GTTAGTGACA
2221TCCTCTTTAA AATATGGGAC TCTTTCCTTT ATGAAGGACC AAAGGTTATT TTCCGTTTTG
2281CTCTGGCACT TTTTAAGTAC AAGGAAGAGG AGATTTTGAA ATTGCAAGAT TCGATGTCTA
2341TATTTAAGTA TCTCCGCTAC TTCACTCGCA CTATCCTTGA TGCTAGATCC GGAACCGACG
2401CGCCTACCAC TTGGAGAAAG TCCGGCTGGA GCTTGCCAAC TTTCTTGTAC AAAGTGGTTG
2461ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2521GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT
2581CCATATACTT ACAGTTGACG CAACGCGTTA AGTCgacaat caacctctgg attacaaaat
2641ttgtgaaaga tt

Download FASTA (ORF) (Full)