Construct: ORF TRCN0000476871
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005492.1_s317c1
- Derived from:
- ccsbBroadEn_00574
- DNA Barcode:
- CCCACCCTAACGAGCTCGAATCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FOXD4 (2298)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476871
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2298 | FOXD4 | forkhead box D4 | NM_207305.4 | 100% | 100% | |
| 2 | human | 200350 | FOXD4L1 | forkhead box D4 like 1 | NM_012184.4 | 90% | 65.8% | (many diffs) |
| 3 | human | 653404 | FOXD4L6 | forkhead box D4 like 6 | NM_001085476.1 | 84.2% | 78% | (many diffs) |
| 4 | human | 286380 | FOXD4L3 | forkhead box D4 like 3 | NM_199135.4 | 84.2% | 78% | (many diffs) |
| 5 | human | 653427 | FOXD4L5 | forkhead box D4 like 5 | NM_001126334.1 | 83.6% | 77.3% | (many diffs) |
| 6 | human | 349334 | FOXD4L4 | forkhead box D4 like 4 | NM_199244.3 | 83.6% | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1386
- ORF length:
- 1317
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacttgcca agagctgagc gccttcgctc cacaccgcag cgcagcctcc 121 gggactccga tggggaagac ggtaaaatcg atgtcctggg agaggaggaa gatgaagacg 181 aggaggaggc ggcgagccag cagttcctag agcagtcgct ccagccgggg ctgcaggtgg 241 cccggtgggg cggggttgcg cttccccgag agcacatcga gggcggcggc ggcccgagcg 301 acccctcaga gtttggcacc gagttcaggg caccgccaag gtctgcggcg gcctctgaag 361 atgcccggca gccggcaaag cccccctcct cgtacatcgc gctcatcacc atggccatcc 421 tgcaaagccc gcacaagcgc ctcacgctca gcggcatctg cgccttcatt agtgaccgct 481 tcccctacta ccgccgcaag ttccccgcct ggcagaacag catccgccac aacctctcgc 541 tgaacgactg cttcgtcaag atcccccgcg agccgggccg cccaggcaag ggcaactact 601 ggagcctgga ccccgcctcc caggacatgt tcgacaatgg cagctttctc cggcgtagga 661 agcgtttcca gcgccaccaa ccgaccccgg gagcccacct gccccacccc ttccctctac 721 ctgctgcaca cgccgccctg cacaaccccc gcccaggccc tctgcttggg gcccctgccc 781 cgccgcagcc agtcccgggg gcctacccca acaccggccc cgggagacgc ccttacgctc 841 tgctgcaccc gcatcctcct cgctacctac tgctctcggc ccccgcctat gccggggcac 901 cgaagaaagc agaaggcgcg gacctggcga ccccggcacc cttcccgtgc tgcagccctc 961 acttggtcct cagccttggg aggagggcaa gggtctggcg tcgccaccgg gaggcggatg 1021 catctctttc agcattgaga gtatcatgca aggggtCAGG GGAGCGGGTA CAGGGGCTGC 1081 GCAGAGTTTG TCCCCGACCG CGTGGAGCTA CTGCCCCCTG CTCCAGCGAC CGTCAAGCCT 1141 GTCGGACAAT TTTGCAGCAA CAGCAGCGGC ATCAGGAGGA GGACTGCGCC AACGGCTGCG 1201 CTCCCACCAA GGGCGCGGTG CTGGGCGGGC ACCTGTCGGC CGCGTCGGCG CTGCTGCGGT 1261 ATCAGGCGGT GGCAGAGGGC TCTGGGCTGA CATCGCTGGC CGCCCCTTTG GGCGGAGAGG 1321 GGACCTCACC AGTTTTTTTA GTATCGCCCA CGCCCAGTTC CCTGGCCGAG TCCGCAGGGC 1381 CCTCCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCACCCTA ACGAGCTCGA ATCCAACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt