Construct: ORF TRCN0000476888
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004063.1_s317c1
- Derived from:
- ccsbBroadEn_13829
- DNA Barcode:
- TTTCTTTGTTTGGAAACAGATCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CES1 (1066)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476888
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1066 | CES1 | carboxylesterase 1 | NM_001266.5 | 99.6% | 99.2% | (many diffs) |
| 2 | human | 1066 | CES1 | carboxylesterase 1 | NM_001025194.2 | 99.4% | 99.1% | (many diffs) |
| 3 | human | 1066 | CES1 | carboxylesterase 1 | XM_005255774.2 | 99.4% | 99.1% | (many diffs) |
| 4 | human | 1066 | CES1 | carboxylesterase 1 | NM_001025195.2 | 99.2% | 98.9% | (many diffs) |
| 5 | human | 390732 | CES1P2 | carboxylesterase 1 pseudoge... | NR_033740.1 | 72% | (many diffs) | |
| 6 | human | 107987423 | LOC107987423 | liver carboxylesterase 1-like | XM_017030236.2 | 50.2% | 50.2% | 0_1ins843;240_242delGCA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1764
- ORF length:
- 1698
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gctccttgct cttgtcctgg ccactctcgc tgcttccgcg gcttgggggc 121 atccgtcctc gccacctgtg gtggacaccg tgcatggcaa agtgctgggg aagttcgtca 181 gcttagaagg atttgcacag cctgtggcca ttttcctggg aatccctttt gccaagccgc 241 ctcttggacc cctgaggttt actccaccgc agcctgcaga accatggagc tttgtgaaga 301 atgccacctc gtaccctcct atgtgcaccc aagatcccaa ggcggggcag ttactctcag 361 agctatttac aaaccgaaag gagaacattc ctctcaagct ttctgaagac tgtctttacc 421 tcaatattta cactcctgct gacttgacca agaaaaacag gctgccggtg atggtgtgga 481 tccacggagg ggggctgatg gtgggtgcgg catcaaccta tgatgggctg gcccttgctg 541 cccatgaaaa cgtggtggtg gtgaccattc aatatcgcct gggcatctgg ggattcttca 601 gcacagggga tgaacacagc cgggggaact ggggtcacct ggaccaggtg gctgccctgc 661 gctgggtcca ggacaacatt gccagctttg gagggaaccc aggctctgtg accatctttg 721 gagagtcagc gggaggagaa agtgtctctg ttcttgtttt gtctccattg gccaagaatc 781 tcttccaccg ggccatttct gagagtggcg tggccctcac ttctgttctg gtgaagaaag 841 gtgatgtcaa gcccttggct gagcaaattg ctatcactgc tgggtgcaaa accaccacct 901 ctgctgtcat ggttcactgc ctgcgacaga agacggaaga ggagctcttg gagacgacat 961 tgaaaatgaa attcttatct ctggacttac agggagaccc cagagagagt caaccccttc 1021 tgggcactgt gattgatggg atgctgctgc tgaaaacacc tgaagagctt caagctgaaa 1081 ggaatttcca cactgtcccc tacatggtcg gaattaacaa gcaggagttt ggctggttga 1141 ttccaatgtt gatgagctat ccactctccg aagggcaact ggaccagaag acagccatgt 1201 cactcctgtg gaagtcctat ccccttgttt gcattgctaa ggaactgatt ccagaagcca 1261 ctgagaaata cttaggagga acagacgaca ctgtcaaaaa gaaagacctg ttcctggact 1321 tgatagcaga tgtgatgttt ggtgtcccat ctgtgattgt ggcccggaac cacagagatg 1381 ctggagcacc cacctacatg tatgagtttc agtaccgtcc aagcttctca tcagacatga 1441 aacccaagac ggtgatagga gaccacgggg atgagctcTT CTCCGTCTTT GGGGCCCCAT 1501 TTTTAAAAGA GGGTGCCTCA GAAGAGGAGA TCAGACTTAG CAAGATGGTG ATGAAATTCT 1561 GGGCCAACTT TGCTCGCAAT GGAAACCCCA ATGGGGAAGG GCTGCCCCAC TGGCCAGAGT 1621 ACAACCAGAA GGAAGGGTAT CTGCAGATTG GTGCCAACAC CCAGGCGGCC CAGAAGCTGA 1681 AGGACAAAGA AGTAGCTTTC TGGACCAACC TCTTTGCCAA GAAGGCAGTG GAGAAGCCAC 1741 CCCAGACAGA ACACATAGAG CTGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1801 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1861 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TTCTTTGTTT 1921 GGAAACAGAT CTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1981 att