Construct: ORF TRCN0000476921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002440.1_s317c1
- Derived from:
- ccsbBroadEn_13303
- DNA Barcode:
- CTCTTCCACAATTAAGATATCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SBSPON (157869)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476921
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 157869 | SBSPON | somatomedin B and thrombosp... | NM_153225.4 | 98.1% | 98.1% | 1_15del |
| 2 | human | 157869 | SBSPON | somatomedin B and thrombosp... | XM_017013145.1 | 75.7% | 74.9% | (many diffs) |
| 3 | human | 157869 | SBSPON | somatomedin B and thrombosp... | XM_024447081.1 | 54.8% | 47.1% | (many diffs) |
| 4 | human | 157869 | SBSPON | somatomedin B and thrombosp... | XR_928762.2 | 45.4% | 1_126del;521_681del;771_772ins293 | |
| 5 | mouse | 226866 | Sbspon | somatomedin B and thrombosp... | NM_001033288.3 | 85.8% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctgtgcgcg ctgtcgcggc tgtggcccgg ggcccaggcc ggctgcgccg 121 aggccgggcg ctgctgtccc ggccgggacc ccgcctgctt cgcccgcggc tggaggctgg 181 acagggtcta cgggacgtgt ttctgcgacc aagcctgtcg cttcaccggg gactgctgct 241 tcgactacga cagggcgtgc ccagctcgcc cgtgcttcgt gggggaatgg agcccctgga 301 gtggttgtgc agaccagtgc aagcctacaa cccgtgtgcg gaggcgctcg gtgcagcagg 361 agcctcagaa cggcggggcg ccctgcccac ccctggaaga gagagctggc tgcctggagt 421 actccacccc gcagggccag gactgcgggc acacctatgt tcctgccttt ataactacct 481 ctgcattcaa caaggagaga acacgacaag ctacGTCTCC ACACTGGTCT ACACACACAG 541 AGGATGCTGG ATACTGTATG GAGTTTAAGA CAGAGTCCTT GACTCCTCAC TGTGCTCTGG 601 AAAACTGGCC CTTGACTAGA TGGATGCAGT ATCTCCGAGA GGGATACACG GTGTGTGTGG 661 ATTGTCAGCC TCCAGCTATG AACTCTGTGA GCCTTCGTTG TTCTGGAGAT GGCCTGGACT 721 CCGATGGAAA TCAGACTCTC CATTGGCAAG CAATTGGTAA TCCTCGGTGT CAAGGAACTT 781 GGAAAAAAGT TCGGCGAGTA GACCAGTGTT CTTGTCCAGC TGTTCACAGT TTTATTTTTA 841 TATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGACT CTTCCACAAT TAAGATATCT AAACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt