Construct: ORF TRCN0000476936
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009320.1_s317c1
- Derived from:
- ccsbBroadEn_13009
- DNA Barcode:
- CGGTACTGGGGTTGGAATGCGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D27P (96597)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476936
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | NM_001039397.2 | 11.9% | 10.9% | (many diffs) |
| 2 | human | 353149 | TBC1D26 | TBC1 domain family member 26 | NM_178571.4 | 9.7% | 8.4% | (many diffs) |
| 3 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_011523781.2 | 8% | 7.4% | (many diffs) |
| 4 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_024450684.1 | 8% | 7.4% | (many diffs) |
| 5 | human | 96597 | TBC1D27P | TBC1 domain family member 2... | NR_147084.1 | 2.3% | 1_268del;350_3421del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 323
- ORF end:
- 404
- ORF length:
- 81
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA CCATGGAGAT GGATGAGCAC CCGGATATCC TGCCCACCCA 361 GGGGCAAGGC AACATCATCA TTACTAAGTA TGAGCAGGGA CGCTTGCCAA CTTTCTTGTA 421 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 481 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 541 AAAGGACGAC GGTACTGGGG TTGGAATGCG CACACGCGTT AAGTCgacaa tcaacctctg 601 gattacaaaa tttgtgaaag att