Construct: ORF TRCN0000476979
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002798.1_s317c1
- Derived from:
- BRDN0000391308
- DNA Barcode:
- GGAATTTGTCACGGTGGCAGCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HGF (3082)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476979
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3082 | HGF | hepatocyte growth factor | NM_000601.6 | 99.7% | 99.4% | (many diffs) |
| 2 | human | 3082 | HGF | hepatocyte growth factor | XM_006715956.2 | 99.7% | 99.4% | (many diffs) |
| 3 | human | 3082 | HGF | hepatocyte growth factor | NM_001010932.3 | 99% | 98.7% | (many diffs) |
| 4 | human | 3082 | HGF | hepatocyte growth factor | XM_011516115.2 | 99% | 98.7% | (many diffs) |
| 5 | human | 3082 | HGF | hepatocyte growth factor | NM_001010931.3 | 39.6% | 39.2% | (many diffs) |
| 6 | human | 3082 | HGF | hepatocyte growth factor | XM_017012097.1 | 39.6% | 39.2% | (many diffs) |
| 7 | human | 3082 | HGF | hepatocyte growth factor | NM_001010933.3 | 38.9% | 38.5% | (many diffs) |
| 8 | human | 3082 | HGF | hepatocyte growth factor | XM_017012098.1 | 38.9% | 38.5% | (many diffs) |
| 9 | human | 3082 | HGF | hepatocyte growth factor | NM_001010934.3 | 28.6% | 28.2% | (many diffs) |
| 10 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289458.1 | 87.6% | 89.8% | (many diffs) |
| 11 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289459.1 | 87.6% | 89.8% | (many diffs) |
| 12 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_010427.5 | 87.6% | 89.8% | (many diffs) |
| 13 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289461.1 | 86.9% | 89.1% | (many diffs) |
| 14 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289460.2 | 25.3% | 25.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2253
- ORF length:
- 2184
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgggtgacc aaactcctgc caggcctgct gtcgcagcat gtcctcctgc 121 atctcctcct gctccccatc gccatcccct atgcagaggg acaaaggaaa agaagaaata 181 caattcatga attcaaaaaa tcagcaaaga ctaccctaat caaaatagat ccagcactga 241 agataaaaac caaaaaagtg aatactgcag accaatgtgc taatagatgt actaggaata 301 aaggacttcc attcacttgc aaggcttttg tttttgataa agcaagaaaa caatgcctct 361 ggttcccctt caatagcatg tcaagtggag tgaaaaaaga atttggccat gaatttgacc 421 tctatgaaaa caaagactac attagaaact gcatcattgg taaaggacgc agctacaagg 481 gaacagtatc tatcactaag agtggcatca aatgtcagcc ctggagttcc atgataccac 541 acgaacacag ctttttgcct tcgagctatc ggggtaaaga cctacaggaa aactactgtc 601 gaaatcctcg aggggaagaa gggggaccct ggtgtttcac aagcaatcca gaggtacgct 661 acgaagtctg tgacattcct cagtgttcag aagttgaatg catgacctgc aatggggaga 721 gttatcgagg tctcatggat catacagaat caggcaagat ttgtcagcgc tgggatcatc 781 agacaccaca ccggcacaaa ttcttgcctg aaagatatcc cgacaagggc tttgatgata 841 attattgccg caatcccgat ggccagccga ggccatggtg ctatactctt gaccctcaca 901 cccgctggga gtactgtgca attaaaacat gcgctgacaa tactatgaat gacactgatg 961 ttcctttgga aacaactgaa tgcatccaag gtcaaggaga aggctacagg ggcactgtca 1021 ataccatttg gaatggaatt ccatgtcagc gttgggattc tcagtatcct cacgagcatg 1081 acatgactcc tgaaaatttc aagtgcaagg acctacgaga aaattactgc cgaaatccag 1141 atgggtctga atcaccctgg tgttttacca ctgatccaaa catccgagtt ggctactgct 1201 cccaaattcc aaactgtgat atgtcacatg gacaagattg ttatcgtggg aatggcaaaa 1261 attatatggg caacttatcc caaacaagat ctggactaac atgttcaatg tgggacaaga 1321 acatggaaga cttacatcgt catatcttct gggaaccaga tgcaagtaag ctgaatgaga 1381 attactgccg aaatccagat gatgatgctc atggaccctg gtgctacacg ggaaatccac 1441 tcattccttg ggattattgc cctatttctc gttgtgaagg tgataccaca cctacaatag 1501 tcaatttaga ccatcccgta atatcttgtg ccaaaacgaa acaattgcga gttgtaaatg 1561 ggattccaac acgaacaaac ataggatgga tggttagttt gagatacaga aataaacata 1621 tctgcggagg atcattgata aaggagagtt gggttcttac tgcacgacag tgtttccctt 1681 ctcgagactt gaaagattat gaagcttggc ttggaattca tgatgtccac ggaagaggag 1741 atgagaaatg caaacaggtt ctcaatgttt cccagctggt atatggccct gaaggatcag 1801 atctggtttt aatgactcct gccaggccTG CTGTCCTGGA TGATTTTGTT AGTACGATTG 1861 ATTTACCTAA TTATGGATGC ACAATTCCTG AAAAGACCAG TTGCAGTGTT TATGGCTGGG 1921 GCTACACTGG ATTGATCAAC TATGATGGCC TATTACGAGT GGCACATCTC TATATAATGG 1981 GAAATGAGAA ATGCAGCCAG CATCATCGAG GGAAGGTGAC TCTGAATGAG TCTGAAATAT 2041 GTGCTGGGGC TGAAAAGATT GGATCAGGAC CATGTGAGGG GGATTATGGT GGCCCACTTG 2101 TTTGTGAGCA ACATAAAATG AGAATGGTTC TTGGTGTCAT TGTTCCTGGT CGTGGATGTG 2161 CCATTCCAAA TCGTCCTGGT ATTTTTGTCC GAGTAGCATA TTATGCAAAA TGGATACACA 2221 AAATTATTTT AACATATAAG GTACCACAGT CATTGCCAAC TTTCTTGTAC AAAGTGGTTG 2281 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2341 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 2401 AATTTGTCAC GGTGGCAGCA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2461 ttgtgaaaga tt