Construct: ORF TRCN0000476992
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012701.1_s317c1
- Derived from:
- ccsbBroadEn_14293
- DNA Barcode:
- TTGCGATTATTTGTACTGCCTCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- KRTAP1-1 (81851)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476992
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81851 | KRTAP1-1 | keratin associated protein 1-1 | NM_030967.3 | 99.6% | 22% | 117_118insT;132_133insA |
| 2 | human | 81850 | KRTAP1-3 | keratin associated protein 1-3 | NM_030966.2 | 89.4% | 20.9% | (many diffs) |
| 3 | human | 83895 | KRTAP1-5 | keratin associated protein 1-5 | NM_031957.2 | 81.1% | 18.9% | (many diffs) |
| 4 | human | 728255 | KRTAP1-4 | keratin associated protein 1-4 | NM_001257305.1 | 62.8% | 19% | (many diffs) |
| 5 | mouse | 75586 | Krtap9-3 | keratin associated protein 9-3 | NM_029351.2 | 63.3% | 23.5% | (many diffs) |
| 6 | mouse | 69664 | Krtap1-5 | keratin associated protein 1-5 | NM_027157.3 | 60.7% | 27% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 189
- ORF length:
- 120
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctgctgt cagaccagct tctgtggatt tcccagctgc tccaccagtg 121 ggacctgcgg ctccagctgc tgccagccaa gctgctgtga gaccagctcc tgccagccac 181 gctgcttgtg agaccagctg catgccagcc aagctgctgc cagaccagct tctgtggatt 241 tcctagcttc TCAACCGGTG GGACTTGTGA CTCTAGCTGC TGCCAGCCAA GCTGCTGTGA 301 AACTAGCTGC TGCCAGCCAA GCTGCTACCA GACCAGCTCC TGCGGAACTG GCTGTGGCAT 361 TGGTGGTGGC ATTGGCTATG GCCAGGAGGG CAGCAGTGGA GCTGTGAGCA CCCGTATCAG 421 GTGGTGCCGC CCAGACTGCC GTGTGGAGGG TACCTGCCTG CCCCCCTGCT GCGTGGTGAG 481 CTGCACACCC CCATCCTGCT GCCAGCTGCA CCACGCCGAG GCCTCCTGCT GCCGCCCATC 541 CTACTGTGGA CAGTCCTGTT GCCGCCCAGT CTGCTGCTGC TACTGCTCTG AGCCCACTTG 601 TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT 661 CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG 721 GCTTTATATA TCTTGTGGAA AGGACGATTG CGATTATTTG TACTGCCTCT CACGCGTTAA 781 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t