Construct: ORF TRCN0000476992

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012701.1_s317c1
Derived from:
ccsbBroadEn_14293
DNA Barcode:
TTGCGATTATTTGTACTGCCTCTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
KRTAP1-1 (81851)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476992
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81851 KRTAP1-1 keratin associated protein 1-1 NM_030967.3 99.6% 22% 117_118insT;132_133insA
2 human 81850 KRTAP1-3 keratin associated protein 1-3 NM_030966.2 89.4% 20.9% (many diffs)
3 human 83895 KRTAP1-5 keratin associated protein 1-5 NM_031957.2 81.1% 18.9% (many diffs)
4 human 728255 KRTAP1-4 keratin associated protein 1-4 NM_001257305.1 62.8% 19% (many diffs)
5 mouse 75586 Krtap9-3 keratin associated protein 9-3 NM_029351.2 63.3% 23.5% (many diffs)
6 mouse 69664 Krtap1-5 keratin associated protein 1-5 NM_027157.3 60.7% 27% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
189
ORF length:
120
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcctgctgt cagaccagct tctgtggatt tcccagctgc tccaccagtg
121ggacctgcgg ctccagctgc tgccagccaa gctgctgtga gaccagctcc tgccagccac
181gctgcttgtg agaccagctg catgccagcc aagctgctgc cagaccagct tctgtggatt
241tcctagcttc TCAACCGGTG GGACTTGTGA CTCTAGCTGC TGCCAGCCAA GCTGCTGTGA
301AACTAGCTGC TGCCAGCCAA GCTGCTACCA GACCAGCTCC TGCGGAACTG GCTGTGGCAT
361TGGTGGTGGC ATTGGCTATG GCCAGGAGGG CAGCAGTGGA GCTGTGAGCA CCCGTATCAG
421GTGGTGCCGC CCAGACTGCC GTGTGGAGGG TACCTGCCTG CCCCCCTGCT GCGTGGTGAG
481CTGCACACCC CCATCCTGCT GCCAGCTGCA CCACGCCGAG GCCTCCTGCT GCCGCCCATC
541CTACTGTGGA CAGTCCTGTT GCCGCCCAGT CTGCTGCTGC TACTGCTCTG AGCCCACTTG
601TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT
661CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG
721GCTTTATATA TCTTGTGGAA AGGACGATTG CGATTATTTG TACTGCCTCT CACGCGTTAA
781GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)