Transcript: Human NM_030966.2

Homo sapiens keratin associated protein 1-3 (KRTAP1-3), mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
KRTAP1-3 (81850)
Length:
991
CDS:
54..557

Additional Resources:

NCBI RefSeq record:
NM_030966.2
NBCI Gene record:
KRTAP1-3 (81850)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_030966.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000159135 GATTCCTTTCAATTCCAGAAA pLKO.1 714 3UTR 100% 4.950 6.930 N KRTAP1-3 n/a
2 TRCN0000160706 CAAGCACTTATGGACAACGAA pLKO.1 626 3UTR 100% 3.000 4.200 N KRTAP1-3 n/a
3 TRCN0000425092 TTCCATTCATGAACGAATTAT pLKO_005 600 3UTR 100% 15.000 10.500 N KRTAP1-3 n/a
4 TRCN0000428664 TTCAACCTTTCTTTGTCTTTC pLKO_005 654 3UTR 100% 10.800 7.560 N KRTAP1-3 n/a
5 TRCN0000161183 GCACTTATGGACAACGAACAA pLKO.1 629 3UTR 100% 4.950 3.465 N KRTAP1-3 n/a
6 TRCN0000417173 TTCAACTTGAAATTTCCACTT pLKO_005 575 3UTR 100% 4.050 2.835 N KRTAP1-3 n/a
7 TRCN0000163496 GCGGATTTCCTAGCTTCTCAA pLKO.1 187 CDS 100% 4.950 2.970 N KRTAP1-3 n/a
8 TRCN0000159076 GATTTCCTAGCTTCTCAACTA pLKO.1 190 CDS 100% 4.950 2.475 Y KRTAP1-3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_030966.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14293 pDONR223 100% 89.4% 20.9% None (many diffs) n/a
2 ccsbBroad304_14293 pLX_304 0% 89.4% 20.9% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000476992 TTGCGATTATTTGTACTGCCTCTC pLX_317 65.1% 89.4% 20.9% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_14300 pDONR223 100% 87.9% 18.5% None (many diffs) n/a
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