Construct: ORF TRCN0000477014
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014977.1_s317c1
- Derived from:
- ccsbBroadEn_09143
- DNA Barcode:
- AAGGTTTATCCCAAGTATCTGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRIP1 (83990)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477014
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | NM_032043.3 | 99.9% | 99.9% | 2637A>G;2755T>C;3411T>C |
| 2 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_011525335.3 | 98.5% | 97.8% | (many diffs) |
| 3 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_011525332.3 | 98.3% | 98.3% | (many diffs) |
| 4 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_011525333.3 | 98.3% | 98.3% | (many diffs) |
| 5 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_011525334.2 | 98.3% | 98.3% | (many diffs) |
| 6 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_011525336.2 | 98.3% | 98.3% | (many diffs) |
| 7 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_011525337.2 | 96.1% | 96.1% | (many diffs) |
| 8 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_017025200.1 | 87% | 86.4% | (many diffs) |
| 9 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_011525338.2 | 85.6% | 85.1% | (many diffs) |
| 10 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_017025201.1 | 83.2% | 82.4% | (many diffs) |
| 11 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_011525339.3 | 65.7% | 61.8% | (many diffs) |
| 12 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_011525340.3 | 65.6% | 65.4% | (many diffs) |
| 13 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_011525341.3 | 52.3% | 51.8% | (many diffs) |
| 14 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_017025202.1 | 49.2% | 48.5% | (many diffs) |
| 15 | human | 83990 | BRIP1 | BRCA1 interacting protein C... | XM_017025203.1 | 49.2% | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3816
- ORF length:
- 3747
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcttcaatg tggtctgaat atacaattgg tggggtgaag atttactttc 121 cttataaagc ttacccgtca cagcttgcta tgatgaattc tattctcaga ggattaaaca 181 gcaagcaaca ttgtttgttg gagagtccca caggaagtgg aaaaagctta gccttacttt 241 gttctgcttt agcatggcaa caatctctta gtgggaaacc agcagatgag ggcgtaagtg 301 aaaaagctga agtacaattg tcatgttgtt gtgcatgcca ttcaaaggat tttacaaaca 361 atgacatgaa ccaaggaact tcacgtcatt tcaactatcc aagcacacca ccttctgaaa 421 gaaatggcac ttcatcaact tgtcaagact cccctgaaaa aaccactctg gctgcaaagt 481 tatctgctaa gaaacaggca tccatataca gagatgaaaa tgatgatttt caagtagaga 541 agaaaagaat tcgaccctta gaaactacac agcagattag aaaacgtcat tgctttggaa 601 cagaagtaca caatttggat gcaaaagttg attcaggaaa gactgtaaaa ctcaactctc 661 cactggaaaa gataaactcc ttttcgccac agaaaccccc tggccactgt tctaggtgct 721 gttgttctac taaacaagga aacagtcaag agtcatcgaa taccattaag aaggatcata 781 cagggaaatc caagataccc aaaatatatt ttgggacacg cacacacaag cagattgctc 841 agattactag agagctccgg aggacggcat attcaggggt tccaatgact attctttcca 901 gcagggatca tacttgtgtc catcctgagg tagtcggtaa cttcaacaga aatgagaagt 961 gcatggaatt gctagatggg aaaaacggaa aatcctgcta tttttatcat ggagttcata 1021 aaattagtga tcagcacaca ttacagactt tccaagggat gtgcaaagcc tgggatatag 1081 aagaacttgt cagcctgggg aagaaactaa aggcctgtcc atattacaca gcccgagaac 1141 taatacaaga tgctgacatc atattttgtc cctacaacta tcttctagat gcacaaataa 1201 gggaaagtat ggatttaaat ctgaaagaac aggttgtcat tttagatgaa gctcataaca 1261 tcgaggactg tgctcgggaa tcagcaagtt acagtgtaac agaagttcag cttcggtttg 1321 ctcgggatga actagatagt atggtcaaca ataatataag gaagaaagat catgaacccc 1381 tacgagctgt gtgctgtagc ctcattaatt ggttagaagc aaacgctgaa tatcttgtag 1441 aaagagatta tgaatcagct tgtaaaatat ggagtggaaa tgaaatgctc ttaactttac 1501 acaaaatggg tatcaccact gctacttttc ccattttgca gggacatttt tctgctgttc 1561 ttcaaaaaga ggaaaaaatc tcaccaattt atggtaaaga ggaggcaaga gaagtacctg 1621 ttattagtgc atcaactcaa ataatgctta aaggactttt tatggtactt gactatcttt 1681 ttaggcaaaa tagcagattt gcagatgatt ataaaattgc gattcaacag acttactcct 1741 ggacaaatca gattgatatt tcagacaaaa atgggttgtt ggttctacca aaaaataaga 1801 aacgttcacg acagaaaact gcagttcatg tgctaaactt ttggtgctta aatccagctg 1861 tggccttttc agatattaat ggcaaagttc agaccattgt tttgacatct ggtacattat 1921 caccaatgaa atccttttcg tcagaacttg gtgttacatt tactatccag ctggaggcta 1981 atcatatcat taaaaattca caggtttggg ttggtaccat tgggtcaggc cccaagggtc 2041 ggaatctctg tgctaccttc cagaatactg aaacatttga gttccaagat gaagtgggag 2101 cacttttgtt atctgtgtgc cagactgtga gccaaggaat tttgtgtttc ttgccatctt 2161 acaagttatt agaaaaatta aaagaacgtt ggctctctac tggtttatgg cataatctgg 2221 agttggtgaa gacagtcatt gtagaaccac agggaggaga aaaaacaaat tttgatgaat 2281 tactgcaggt gtactatgac gcaatcaaat acaaaggaga gaaagatgga gctctcctgg 2341 tagcagtttg tcgtggtaaa gtgagtgagg gtctggattt ctcagatgac aatgcccgtg 2401 ctgtcataac aataggaatt ccttttccaa atgtgaaaga tctacaggtt gaactaaaac 2461 gacaatacaa tgaccaccat tcaaaattga gaggtcttct acctggccgt cagtggtatg 2521 aaattcaagc atacagggcc ttaaaccagg cccttggtag atgtattaga cacagaaatg 2581 attggggagc tcttattcta gtggatgatc gctttaggaa taacccaagt cgctatatat 2641 ctggactttc taaatgggta cggcagcaga ttcagcacca ttcaaccttt gaaagtgcac 2701 tggagtcctt ggctgaattt tccaaaaagc atcaaaaagt tcttaatgta tccataaagg 2761 acagaaccaa tatacaggac aatgagtcta cacttgaagt gacctcttta aagtacagta 2821 ccccacctta tttactggaa gcagcaagtc atctatcacc agaaaatttt gtggaagatg 2881 aagcaaagat atgtgtccag gaactacagt gtcctaaaat tattaccaaa aattcacctc 2941 taccaagtag cattatctcc agaaaggaga aaaatgatcc agtattcctg gaagaagcag 3001 ggaaagcaga aaaaattgtg atttccagat ccacaagccc aactttcaac aaacaaacaa 3061 agagagttag ctggtcaagc tttaattctt tgggacagta ttttactggt aaaataccga 3121 aggcaacacc tgagctcggg tcatcagaga atagtgcctc tagtcctccc cgtttcaaaa 3181 cagagaagat ggaaagtaaa actgttttgc ccttcactga taaatgtgaa tcctcaaatc 3241 tgacagtaaa cacatcgttt ggatcatgcc ctcaatcaga aaccattatt tcatcattaa 3301 agattgatgc cacccttact agaaaaaatc attctgaaca tccgctctgt tctgaagaag 3361 ccctGGATCC AGACATTGAA TTGTCTCTAG TAAGTGAAGA AGATAAACAG TCCACTTCAA 3421 ATAGAGATTT TGAAACAGAA GCAGAAGATG AATCTATCTA TTTTACACCT GAACTTTACG 3481 ATCCTGAAGA TACAGATGAA GAAAAAAATG ACCTAGCTGA AACTGATAGA GGAAATAGAT 3541 TGGCTAACAA TTCAGATTGC ATTTTAGCTA AAGACCTTTT TGAAATTAGA ACTATAAAAG 3601 AAGTAGATTC AGCCAGAGAA GTGAAAGCTG AGGATTGCAT AGATACAAAG TTGAATGGAA 3661 TTCTGCATAT TGAAGAAAGT AAAATTGATG ACATTGATGG TAATGTAAAA ACAACTTGGA 3721 TAAATGAACT GGAACTGGGA AAAACTCATG AAATAGAAAT AAAGAACTTT AAACCATCTC 3781 CTTCCAAAAA TAAAGGCATG TTTCCTGGTT TTAAGTTGCC AACTTTCTTG TACAAAGTGG 3841 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3901 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3961 AAAGGTTTAT CCCAAGTATC TGTGTACGCG TTAAGTCgac aatcaacctc tggattacaa 4021 aatttgtgaa agatt