Transcript: Human NM_032043.3

Homo sapiens BRCA1 interacting protein C-terminal helicase 1 (BRIP1), mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
BRIP1 (83990)
Length:
8182
CDS:
276..4025

Additional Resources:

NCBI RefSeq record:
NM_032043.3
NBCI Gene record:
BRIP1 (83990)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_032043.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000368638 ATAACCCAAGTCGCTATATAT pLKO_005 2827 CDS 100% 15.000 21.000 N BRIP1 n/a
2 TRCN0000358768 ACTAAAGGCCTGTCCATATTA pLKO_005 1313 CDS 100% 15.000 12.000 N BRIP1 n/a
3 TRCN0000049914 CGTCAGAACTTGGTGTTACAT pLKO.1 2146 CDS 100% 5.625 4.500 N BRIP1 n/a
4 TRCN0000358703 CTCAATCAGAAACCATTATTT pLKO_005 3478 CDS 100% 15.000 10.500 N BRIP1 n/a
5 TRCN0000049916 CCAAGGAACTTCACGTCATTT pLKO.1 578 CDS 100% 13.200 9.240 N BRIP1 n/a
6 TRCN0000049913 GCTAAGAAACAGGCATCCATA pLKO.1 693 CDS 100% 4.950 3.465 N BRIP1 n/a
7 TRCN0000049915 GCTCTTATTCTAGTGGATGAT pLKO.1 2796 CDS 100% 4.950 3.465 N BRIP1 n/a
8 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 6562 3UTR 100% 4.950 2.475 Y ERAP2 n/a
9 TRCN0000008902 CCTCCCAAAGTGTTGGGATTA pLKO.1 7215 3UTR 100% 1.080 0.540 Y GPR83 n/a
10 TRCN0000156315 CCTCCCAAAGTGTTGGGATTA pLKO.1 7215 3UTR 100% 1.080 0.540 Y MYORG n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 6563 3UTR 100% 13.200 6.600 Y LIAS n/a
12 TRCN0000130146 CAGGTTCAAGTGATTCTCCTA pLKO.1 4264 3UTR 100% 2.640 1.320 Y DICER1-AS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_032043.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09143 pDONR223 100% 99.9% 99.9% None 2637A>G;2755T>C;3411T>C n/a
2 ccsbBroad304_09143 pLX_304 0% 99.9% 99.9% V5 2637A>G;2755T>C;3411T>C n/a
3 TRCN0000477014 AAGGTTTATCCCAAGTATCTGTGT pLX_317 12.2% 99.9% 99.9% V5 2637A>G;2755T>C;3411T>C n/a
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