Construct: ORF TRCN0000477016
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014568.1_s317c1
- Derived from:
- ccsbBroadEn_07689
- DNA Barcode:
- CCGGCCGTAATACAAAGCCAGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBGCP2 (10844)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477016
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10844 | TUBGCP2 | tubulin gamma complex assoc... | NM_006659.3 | 99.8% | 99.8% | 1941C>T;2097T>C;2677C>A |
| 2 | human | 10844 | TUBGCP2 | tubulin gamma complex assoc... | NM_001256617.1 | 96.8% | 96.8% | (many diffs) |
| 3 | human | 10844 | TUBGCP2 | tubulin gamma complex assoc... | NM_001256618.1 | 85.4% | 85.4% | (many diffs) |
| 4 | human | 10844 | TUBGCP2 | tubulin gamma complex assoc... | NR_046330.1 | 57.9% | (many diffs) | |
| 5 | mouse | 74237 | Tubgcp2 | tubulin, gamma complex asso... | NM_001286007.1 | 85.9% | 90.8% | (many diffs) |
| 6 | mouse | 74237 | Tubgcp2 | tubulin, gamma complex asso... | NM_133755.2 | 85.9% | 90.8% | (many diffs) |
| 7 | mouse | 74237 | Tubgcp2 | tubulin, gamma complex asso... | NM_001286009.1 | 75% | 79.1% | (many diffs) |
| 8 | mouse | 74237 | Tubgcp2 | tubulin, gamma complex asso... | XM_011249861.2 | 60.2% | 63.5% | (many diffs) |
| 9 | mouse | 74237 | Tubgcp2 | tubulin, gamma complex asso... | NM_001286011.1 | 57.9% | 61.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2775
- ORF length:
- 2706
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtgaattt cggattcacc atgacgtcaa tgaactgctt agcctgctgc 121 gtgtccacgg aggagatggg gctgaggtct acattgacct gcttcaaaag aacaggaccc 181 cgtacgtcac taccactgtc tctgctcaca gtgccaaggt taaaattgca gagttttctc 241 gtactccaga agactttcta aagaaatatg atgaactgaa atctaaaaat acaaggaacc 301 ttgacccgct ggtgtacctg ttgtcaaagc tcacggaaga caaagagact ctgcagtact 361 tacaacagaa tgcaaaagaa agagctgagc ttgcagccgc tgctgtgggc agcagtacca 421 ccagcatcaa cgtccctgcc gcggcctcca agatctccat gcaggagctt gaggaactga 481 ggaagcagct tggcagcgtg gccacaggct ccacgctgca gcagtctctg gaacttaaaa 541 gaaagatgct tcgagacaag cagaacaaaa aaaattcagg ccagcacctc cccatcttcc 601 cagcatgggt gtatgagaga cctgccctga tcggggattt cctgattggt gctggcatca 661 gcacagacac cgctttgccg ataggcacgt tgcccctggc ctcgcaggag tcggccgtgg 721 tggaggacct gctgtacgtg ctggtgggcg tggacgggag gtacgtcagt gctcagcccc 781 tggctgggag gcagagccgg accttcctcg tggaccccaa cctggacctg tccatcaggg 841 agctggtgca caggatcctc ccagtggccg ccagctactc cgctgtgacc aggttcattg 901 aagagaagtc ttccttcgag tacgggcagg tgaaccacgc cctggcggcc gccatgcgca 961 ccctggtgaa ggagcacctg attctggtgt cacagctgga gcagctgcac aggcagggcc 1021 tcctttcgct gcagaagctc tggttctaca tccagccagc catgcgcacc atggacatcc 1081 tggcctccct cgccacctcg gtggacaaag gcgaatgtct tggggggtcc acgctgagcc 1141 tgctccacga caggagcttc agctacacag gggacagcca ggcgcaggag ctatgcctgt 1201 acctaaccaa ggcggccagt gctccctact tcgaggttct ggagaagtgg atctacaggg 1261 gcatcatcca cgacccatac agtgagttta tggtcgagga gcacgagctg cggaaggaga 1321 ggatccagga ggattacaac gacaagtact gggaccagcg gtacaccatc gtccagcagc 1381 agatcccgtc cttcctgcag aaaatggcgg acaagatcct cagcacagga aaatatctaa 1441 atgtggtcag agagtgtggc catgacgtca cctgcccggt ggctaaagag atcatctaca 1501 cgttaaaaga gcgggcgtat gtggagcaga tcgagaaggc gtttaactac gccagcaagg 1561 tgctgctgga cttcctgatg gaggagaagg agctggtggc tcacctcagg tccatcaagc 1621 gctacttcct catggaccag ggcgacttct tcgtgcactt catggacctc gcggaggagg 1681 agctccggaa gccggtggag gacatcacgc cccctcgcct ggaagcgctc ctggagctgg 1741 cgctgcgcat gagcacggcc aacactgacc ccttcaagga cgacctcaag atcgacctga 1801 tgccccatga cctcatcact cagctcttgc gcgtcctggc catcgagacc aagcaggaga 1861 aggcgatggc gcacgccgac cccacggagc tggcgctgag cggcctggag gccttctctt 1921 tcgactacat cgtcaagtgg cccctttcgc tcatcatcaa caggaaagcc ctcactcgct 1981 accagatgct cttcaggcac atgttctatt gcaagcacgt ggagcggcag ctctgcagcg 2041 tctggatcag caacaaaacc gccaagcagc actcgctgca ctccgcccag tggtttgctg 2101 gggctttcac tctgcggcag cgaatgctca acttcgtcca gaatattcaa tactacatga 2161 tgttcgaagt gatggaaccg acctggcaca tcctggagaa aaacctgaaa tccgcctcca 2221 acattgacga cgtccttggc caccacacag gcttcctgga cacctgcctg aaggactgca 2281 tgctcaccaa ccccgagctg ctgaaggTCT TCTCCAAGCT CATGTCTGTG TGCGTCATGT 2341 TCACCAACTG CATGCAGAAA TTTACACAGA GCATGAAATT AGATGGCGAG CTGGGCGGGC 2401 AGACGCTGGA GCACAGCACC GTCCTGGGGC TGCCCGCAGG GGCCGAGGAG CGGGCCCGGA 2461 AGGAGCTCGC CAGGAAGCAC CTGGCTGAGC ACGCAGACAC TGTGCAGCTG GTGTCCGGCT 2521 TCGAGGCCAC CATCAACAAG TTTGACAAGA ACTTCTCAGC CCACCTGCTG GACCTCCTGG 2581 CCCGGCTGAG CATCTATAGC ACCAGTGACT GTGAGCACGG CATGGCCAGC GTCATCTCCA 2641 GGCTTGACTT CAATGGTTTC TACACGGAGC GCCTGGAGCG CCTGTCTGCA GAGAGGAGCC 2701 AGAAGGCCAC CCCCCAAGTG CCTGTCCTGC GGGGGCCCCC GGCTACTGCA CCCAGGGTCG 2761 CAGTCACCGC ACAGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2821 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2881 TTTCGGTTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCGGCCGTAA TACAAAGCCA 2941 GACCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt