Construct: ORF TRCN0000477057
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002544.1_s317c1
- Derived from:
- ccsbBroadEn_03835
- DNA Barcode:
- CTAGTAGACCTTGTTCCTCTTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLAIN2 (57606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477057
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57606 | SLAIN2 | SLAIN motif family member 2 | NM_020846.2 | 100% | 100% | |
| 2 | human | 57606 | SLAIN2 | SLAIN motif family member 2 | XM_005248121.3 | 95.7% | 95.5% | 1361_1438del |
| 3 | mouse | 75991 | Slain2 | SLAIN motif family, member 2 | NM_001113423.2 | 90.8% | 93.9% | (many diffs) |
| 4 | mouse | 75991 | Slain2 | SLAIN motif family, member 2 | NM_153567.3 | 87% | 89.8% | (many diffs) |
| 5 | mouse | 75991 | Slain2 | SLAIN motif family, member 2 | XM_006504171.1 | 66.2% | 68.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1812
- ORF length:
- 1743
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggacgtt aactccaacg tgaacgcgga ccaggaggtg cggaagctgc 121 aggagctggt gaagaagctg gagaagcaga acgaacagct gaggagccgc tcgggggccg 181 tgcagggcgc cggctccctt gggcccggca gcccggttcg ggccggcgcg tccattccct 241 cctccggcgc ggcgtctcct cggggcttcc ccttgggcct cagcgccaag tcgggcggcg 301 ggcccgggtc gggcccgagg cggacgagta gcgaagagct gcgggacgcc acctccttgc 361 tagcggcggg cgagggcggc ttgctggacg aggtggagcc gctgcggccc gacgagctgg 421 agcgcctgtc aggctgggag gaggaggagg agagctggct gtattcatca ccaaagaaaa 481 aacttacacc aatgcagaaa tcggttagtc cattagtttg gtgcaggcaa gttttggatt 541 acccaagtcc tgatgttgag tgtgctaaaa aatctcttat ccacaaactt gatcaaacta 601 tgtcagctct caagaggcag aatttatata ataatccttt caactctatg agttacacca 661 gtccttacag tccaaatgcc agtagcccat acagcagtgg cttcaattct ccatcctcaa 721 ccccagtgcg acctcctata gtcaaacagc ttatacttcc tggaaattca ggtaacttga 781 aaagctcaga cagaaatcct ccactcagtc ctcagtcctc tatagatagt gagttaagtg 841 cttcagaatt agatgaagat tcaattggat ccaattataa gctaaatgat gtaactgatg 901 tacagattct agcccggatg caggaagaaa gtctccggca agaatatgca gccaccacgt 961 ctcggcgcag ttctggttca tcttgcaatt ctacaagacg gggtactttt agtgatcagg 1021 aacttgatgc acaaagttta gatgatgaag atgacaatat gcatcatgca gtataccctg 1081 ctgttaacag gttttcacca tcaccacgca attcacctcg accgtcacct aagcagtcac 1141 ccagaaattc acctcgttca cgatctcctg ctcggggaat agaatatagt agagtgtccc 1201 cacagcctat gattagccgc ttacagcaac ctcgcctttc acttcaaggc catcccacag 1261 atttacagac aagcaatgtt aaaaatgaag aaaaactaag acgcagtctt ccaaacctgt 1321 cccgaacatc taatacacaa gttgactcag tgaaaagcag cagaagtgac tcaaattttc 1381 aagtgccaaa cggaggaata cctcgtatgc aacctcaggc ttcagccata ccttctccag 1441 gcaaattccg ttccCCTGCA GCACCATCTC CTTTGGCTCT TCGGCAACCA GTGAAAGCAT 1501 TTAGTAACCA TGGCTCTGGT TCTCCTGGTA GCCAAGAAAT AACACAGCTC ACACAAACCA 1561 CCTCCTCACC TGGGCCTCCT ATGGTTCAGA GCACAGTCTC AGCAAATCCT CCCAGCAATA 1621 TCAACAGCGC TACTCTAACC AGACCTGCAG GGACAACTGC AATGAGAAGT GGCTTGCCCA 1681 GACCCAGTGC CCCTTCTGCT GGGGGCATAC CAGTGCCTCG CAGCAAACTT GCACAGCCTG 1741 TTCGCAGATC CTTGCCAGCT CCTAAAACCT ATGGTAGCAT GAAAGATGAC AGTTGGAAAG 1801 ATGGCTGTTA CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1861 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1921 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTA GTAGACCTTG TTCCTCTTGG 1981 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t