Construct: ORF TRCN0000477065
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012250.1_s317c1
- Derived from:
- ccsbBroadEn_09964
- DNA Barcode:
- TTGGAGACCAACTTCGTAATGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A42 (284439)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477065
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284439 | SLC25A42 | solute carrier family 25 me... | NM_001321544.2 | 99.7% | 99.3% | 115T>C;934C>A |
| 2 | human | 284439 | SLC25A42 | solute carrier family 25 me... | NM_178526.5 | 99.7% | 99.3% | 115T>C;934C>A |
| 3 | human | 284439 | SLC25A42 | solute carrier family 25 me... | XM_005259861.3 | 99.7% | 99.3% | 115T>C;934C>A |
| 4 | human | 284439 | SLC25A42 | solute carrier family 25 me... | XM_011527937.2 | 99.7% | 99.3% | 115T>C;934C>A |
| 5 | human | 284439 | SLC25A42 | solute carrier family 25 me... | XM_017026670.2 | 83% | 82.7% | 1_192del;307T>C;1126C>A |
| 6 | mouse | 73095 | Slc25a42 | solute carrier family 25, m... | NM_001007570.2 | 85.6% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg taatggtgtg aaggaaggcc cggtgcgatt gcatgaggat gctgaggctg 121 tcctgtcctc gtccgtctca tcaaagcgtg accacaggca agtgctcagc tccctgctgc 181 ctggggccct ggctggtgcc cttgccaaaa cagcggtagc tcccctggac cgaaccaaaa 241 tcatcttcca agtgtcttca aaaagatttt ctgccaagga ggccttccgg gtcctctact 301 acacctacct caacgaggga tttctcagct tgtggcgcgg gaactcggcc accatggtgc 361 gcgtggtgcc ctacgccgcc atccagttca gcgcacacga ggagtacaag cgcatcctgg 421 gcagctacta tggcttccgt ggagaagccc tgcccccttg gcctcgcctc ttcgccggcg 481 cactggctgg aacgacagcc gcttcactga cctaccccct ggacctggtc agagcgcgga 541 tggccgtaac cccgaaggaa atgtacagca acatctttca tgtcttcatc cgcatctcga 601 gagaagaggg gctgaagact ctctaccatg gatttatgcc caccgtgctg ggggtcattc 661 cctacgctgg ccTGAGCTTC TTCACCTATG AGACGCTCAA GAGCTTGCAC AGAGAGTACA 721 GCGGCCGCCG GCAGCCCTAC CCCTTCGAGC GCATGATCTT CGGCGCCTGC GCTGGCCTCA 781 TCGGGCAGTC GGCCTCGTAC CCGCTGGATG TGGTGCGGCG GCGCATGCAG ACGGCCGGCG 841 TCACGGGCTA CCCGCGCGCC TCCATCGCCC GCACGCTGCG CACCATCGTG CGGGAGGAGG 901 GCGCCGTGCG CGGCCTCTAC AAAGGCTTGA GCATGAACTG GGTCAAGGGT CCCATCGCCG 961 TGGGCATCAG CTTCACCACC TTCGACCTCA TGCAGATCAT GCTGCGGCAC CTGCAGAGCT 1021 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGATTGGA GACCAACTTC GTAATGTTGA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt