Construct: ORF TRCN0000477066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002875.1_s317c1
- Derived from:
- ccsbBroadEn_05134
- DNA Barcode:
- CCCCCTGAGCGTAGACCATACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBXN2A (165324)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477066
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 165324 | UBXN2A | UBX domain protein 2A | NM_181713.4 | 100% | 100% | |
| 2 | human | 165324 | UBXN2A | UBX domain protein 2A | XM_005264166.5 | 100% | 100% | |
| 3 | human | 165324 | UBXN2A | UBX domain protein 2A | XM_005264167.5 | 100% | 100% | |
| 4 | human | 165324 | UBXN2A | UBX domain protein 2A | XM_005264168.5 | 100% | 100% | |
| 5 | human | 165324 | UBXN2A | UBX domain protein 2A | XM_005264169.4 | 100% | 100% | |
| 6 | human | 165324 | UBXN2A | UBX domain protein 2A | XM_011532633.3 | 100% | 100% | |
| 7 | human | 165324 | UBXN2A | UBX domain protein 2A | XM_017003518.1 | 76.9% | 76.8% | 0_1ins178;2delT |
| 8 | human | 165324 | UBXN2A | UBX domain protein 2A | XM_024452738.1 | 76.9% | 76.8% | 0_1ins178;2delT |
| 9 | human | 165324 | UBXN2A | UBX domain protein 2A | XM_017003517.2 | 76.3% | 74.9% | (many diffs) |
| 10 | mouse | 217379 | Ubxn2a | UBX domain protein 2A | NM_145441.3 | 86.3% | 83.9% | (many diffs) |
| 11 | mouse | 217379 | Ubxn2a | UBX domain protein 2A | XR_381186.3 | 13.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 846
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaagacgta gataacctca aaagtataaa agaagaatgg gtttgtgaaa 121 caggatctga taatcaacct cttggtaata atcaacaatc aaattgtgaa tattttgttg 181 atagcctttt tgaggaagct cagaaggtta gttccaaatg tgtgtctccc gctgaacaga 241 agaaacaggt agatgtaaat ataaaattat ggaaaaacgg attcaccgtc aacgacgatt 301 tcagaagtta ttccgatggt gccagtcagc agtttttgaa ctccatcaaa aagggggaat 361 taccttcaga attacaggga atttttgata aagaagaggt ggacgttaaa gttgaagaca 421 agaaaaatga aatatgtttg tcTACGAAGC CTGTGTTCCA GCCCTTTTCA GGACAGGGTC 481 ACAGACTAGG AAGTGCCACA CCAAAAATTG TTTCTAAAGC AAAGAATATT GAAGTTGAAA 541 ATAAAAATAA TTTGTCTGCT GTTCCACTGA ACAACTTGGA ACCCATTACT AATATACAGA 601 TCTGGTTGGC CAATGGAAAA AGGATTGTCC AGAAATTTAA CATTACTCAT AGAGTAAGCC 661 ATATCAAAGA CTTCATTGAA AAATACCAAG GATCTCAAAG AAGTCCTCCG TTTTCCCTGG 721 CAACAGCTCT TCCTGTCCTC AGGTTGCTAG ATGAGACACT CACACTGGAA GAAGCAGATT 781 TACAGAATGC TGTCATCATT CAGAGACTCC AAAAAACTGC ATCTTTTAGA GAACTTTCAG 841 AGCACTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 901 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 961 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCCCTGAG CGTAGACCAT ACTCAACGCG 1021 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt