Construct: ORF TRCN0000477122
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013996.1_s317c1
- Derived from:
- ccsbBroadEn_00029
- DNA Barcode:
- ACACGGTGCCTTAGCACTTCCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADH7 (131)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477122
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 131 | ADH7 | alcohol dehydrogenase 7 (cl... | NM_000673.4 | 100% | 100% | |
| 2 | human | 131 | ADH7 | alcohol dehydrogenase 7 (cl... | NM_001166504.1 | 95.6% | 92.2% | (many diffs) |
| 3 | mouse | 11529 | Adh7 | alcohol dehydrogenase 7 (cl... | NM_009626.4 | 82.9% | 86.2% | (many diffs) |
| 4 | mouse | 11529 | Adh7 | alcohol dehydrogenase 7 (cl... | XM_006500919.3 | 82.9% | 86.2% | (many diffs) |
| 5 | mouse | 11529 | Adh7 | alcohol dehydrogenase 7 (cl... | XM_006500920.2 | 82% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1227
- ORF length:
- 1158
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttgcagaa atacagatcc aagacaaaga caggatgggc actgctggaa 121 aagttattaa atgcaaagca gctgtgcttt gggagcagaa gcaacccttc tccattgagg 181 aaatagaagt tgccccacca aagactaaag aagttcgcat taagattttg gccacaggaa 241 tctgtcgcac agatgaccat gtgataaaag gaacaatggt gtccaagttt ccagtgattg 301 tgggacatga ggcaactggg attgtagaga gcattggaga aggagtgact acagtgaaac 361 caggtgacaa agtcatccct ctctttctgc cacaatgtag agaatgcaat gcttgtcgca 421 acccagatgg caacctttgc attaggagcg atattactgg tcgtggagta ctggctgatg 481 gcaccaccag atttacatgc aagggcaaac cagtccacca cttcatgaac accagtacat 541 ttaccgagta cacagtggtg gatgaatctt ctgttgctaa gattgatgat gcagctcctc 601 ctgagaaagt ctgtttaatt ggctgtgggt tttccactgg atatggcgct gctgttaaaa 661 ctggcaaggt caaacctggt tccacttgcg tcgtctttgg cctgggagga gttggcctgt 721 cagtcatcat gggctgtaag tcagctggtg catctaggat cattgggatt gacctcaaca 781 aagacaaatt tgagaaGGCC ATGGCTGTAG GTGCCACTGA GTGTATCAGT CCCAAGGACT 841 CTACCAAACC CATCAGTGAG GTGCTGTCAG AAATGACAGG CAACAACGTG GGATACACCT 901 TTGAAGTTAT TGGGCATCTT GAAACCATGA TTGATGCCCT GGCATCCTGC CACATGAACT 961 ATGGGACCAG CGTGGTTGTA GGAGTTCCTC CATCAGCCAA GATGCTCACC TATGACCCGA 1021 TGTTGCTCTT CACTGGACGC ACATGGAAGG GATGTGTCTT TGGAGGTTTG AAAAGCAGAG 1081 ATGATGTCCC AAAACTAGTG ACTGAGTTCC TGGCAAAGAA ATTTGACCTG GACCAGTTGA 1141 TAACTCATGT TTTACCATTT AAAAAAATCA GTGAAGGATT TGAGCTGCTC AATTCAGGAC 1201 AAAGCATTCG AACGGTCCTG ACGTTTTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACACGGTG 1381 CCTTAGCACT TCCATGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt