Construct: ORF TRCN0000477131
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001485.1_s317c1
- Derived from:
- ccsbBroadEn_00184
- DNA Barcode:
- CATACACCTCACTTATGATTTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C2 (717)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477131
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 717 | C2 | complement C2 | NM_000063.6 | 100% | 100% | |
| 2 | human | 717 | C2 | complement C2 | NM_001282458.2 | 93.7% | 88.3% | 0_1ins115;142_169del |
| 3 | human | 717 | C2 | complement C2 | NM_001145903.3 | 82.4% | 82.4% | 45_46ins396 |
| 4 | human | 717 | C2 | complement C2 | NM_001178063.2 | 69.4% | 68.1% | (many diffs) |
| 5 | human | 717 | C2 | complement C2 | NM_001282457.2 | 67.2% | 62.8% | 0_1ins505;111_112ins233 |
| 6 | human | 717 | C2 | complement C2 | NM_001282459.2 | 41.3% | 37.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2325
- ORF length:
- 2256
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcccactg atggttcttt tttgcctgct gttcctgtac ccaggtctgg 121 cagactcggc tccctcctgc cctcagaacg tgaatatctc gggtggcacc ttcaccctca 181 gccatggctg ggctcctggg agccttctca cctactcctg cccccagggc ctgtacccat 241 ccccagcatc acggctgtgc aagagcagcg gacagtggca gaccccagga gccacccggt 301 ctctgtctaa ggcggtctgc aaacctgtgc gctgtccagc ccctgtctcc tttgagaatg 361 gcatttatac cccacggctg gggtcctatc ccgtgggtgg caatgtgagc ttcgagtgtg 421 aggatggctt catattgcgg ggctcgcctg tgcgtcagtg tcgccccaac ggcatgtggg 481 atggagaaac agctgtgtgt gataatgggg ctggccactg ccccaaccca ggcatttcac 541 tgggcgcagt gcggacaggc ttccgctttg gtcatgggga caaggtccgc tatcgctgct 601 cctcgaatct tgtgctcacg gggtcttcgg agcgggagtg ccagggcaac ggggtctgga 661 gtggaacgga gcccatctgc cgccaaccct actcttatga cttccctgag gacgtggccc 721 ctgccctggg cacttccttc tcccacatgc ttggggccac caatcccacc cagaagacaa 781 aggaaagcct gggccgtaaa atccaaatcc agcgctctgg tcatctgaac ctctacctgc 841 tcctggactg ttcgcagagt gtgtcggaaa atgactttct catcttcaag gagagcgcct 901 ccctcatggt ggacaggatc ttcagctttg agatcaatgt gagcgttgcc attatcacct 961 ttgcctcaga gcccaaagtc ctcatgtctg tcctgaacga caactcccgg gatatgactg 1021 aggtgatcag cagcctggaa aatgccaact ataaagatca tgaaaatgga actgggacta 1081 acacctatgc ggccttaaac agtgtctatc tcatgatgaa caaccaaatg cgactcctcg 1141 gcatggaaac gatggcctgg caggaaatcc gacatgccat catccttctg acagatggaa 1201 agtccaatat gggtggctct cccaagacag ctgttgacca tatcagagag atcctgaaca 1261 tcaaccagaa gaggaatgac tatctggaca tctatgccat cggggtgggc aagctggatg 1321 tggactggag agaactgaat gagctagggt ccaagaagga tggtgagagg catgccttca 1381 ttctgcagga cacaaaggct ctgcaccagg tctttgaaca tatgctggat gtctccaagc 1441 tcacagacac catctgcggg gtggggaaca tgtcagcaaa cgcctctgac caggagagga 1501 caccctggca tgtcactatt aagcccaaga gccaagagac ctgccggggg gccctcatct 1561 ccgaccaatg ggtcctgaca gcagctcatt gcttccgcga tggcaacgac cactccctgt 1621 ggagggtcaa tgtgggagac cccaaatccc agtggggcaa agaattcctt attgagaagg 1681 cggtgatctc cccagggttt gatgtctttg ccaaaaagaa ccagggaatc ctggagttct 1741 atggtgatga catagctctg ctgaagctgg cccagaaagt aaagatgtcc acccatgcca 1801 ggcccatctg ccttccctgc acgatggagg ccaatctggc tctgcggaga cctcaaggca 1861 gcacctgtag ggaccatgag aatgaactgc tgaacaaaca gagtgttcct gctcattttg 1921 tcgccttgaa tgggagcaaa ctgaacatta aCCTTAAGAT GGGAGTGGAG TGGACAAGCT 1981 GTGCCGAGGT TGTCTCCCAA GAAAAAACCA TGTTCCCCAA CTTGACAGAT GTCAGGGAGG 2041 TGGTGACAGA CCAGTTCCTA TGCAGTGGGA CCCAGGAGGA TGAGAGTCCC TGCAAGGGAG 2101 AATCTGGGGG AGCAGTTTTC CTTGAGCGGA GATTCAGGTT TTTTCAGGTG GGTCTGGTGA 2161 GCTGGGGTCT TTACAACCCC TGCCTTGGCT CTGCTGACAA AAACTCCCGC AAAAGGGCCC 2221 CTCGTAGCAA GGTCCCGCCG CCACGAGACT TTCACATCAA TCTCTTCCGC ATGCAGCCCT 2281 GGCTGAGGCA GCACCTGGGG GATGTCCTGA ATTTTTTACC CCTCTTGCCA ACTTTCTTGT 2341 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2401 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2461 GAAAGGACGA CATACACCTC ACTTATGATT TCAAACGCGT TAAGTCgaca atcaacctct 2521 ggattacaaa atttgtgaaa gatt