Construct: ORF TRCN0000477142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008860.1_s317c1
- Derived from:
- ccsbBroadEn_04376
- DNA Barcode:
- TTTTTGTCGTAGCTTACGCACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACBD6 (84320)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477142
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84320 | ACBD6 | acyl-CoA binding domain con... | NM_032360.4 | 100% | 100% | |
| 2 | human | 84320 | ACBD6 | acyl-CoA binding domain con... | XR_002957801.1 | 12.1% | 1_311del;1158_6986del | |
| 3 | human | 84320 | ACBD6 | acyl-CoA binding domain con... | XR_001737485.2 | 11.9% | 1_311del;1158_7067del | |
| 4 | human | 84320 | ACBD6 | acyl-CoA binding domain con... | XR_001737484.2 | 11.9% | 1_311del;1158_7084del | |
| 5 | human | 84320 | ACBD6 | acyl-CoA binding domain con... | XR_921977.3 | 11.7% | 1_311del;1158_7211del | |
| 6 | human | 84320 | ACBD6 | acyl-CoA binding domain con... | XR_921978.3 | 11.7% | 1_311del;1158_7215del | |
| 7 | mouse | 72482 | Acbd6 | acyl-Coenzyme A binding dom... | NM_028250.3 | 86.4% | 85.8% | (many diffs) |
| 8 | mouse | 72482 | Acbd6 | acyl-Coenzyme A binding dom... | NM_001145781.1 | 41.2% | 38.6% | (many diffs) |
| 9 | mouse | 72482 | Acbd6 | acyl-Coenzyme A binding dom... | NM_026683.1 | 40.7% | 38.9% | (many diffs) |
| 10 | mouse | 72482 | Acbd6 | acyl-Coenzyme A binding dom... | XR_387197.3 | 36% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 912
- ORF length:
- 846
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcatcattc ctgcccgcgg gggccatcac cggcgacagc ggtggagagc 121 tgagctcagg ggacgactcc ggggaggtgg agttccccca tagccctgag atcgaggaga 181 ccagttgcct ggccgagctg tttgagaagg ctgccgcgca cctgcaaggc ctgattcagg 241 tggccagcag ggagcagctc ttgtacctgt atgccaggta caaacaggtc aaagttggaa 301 attgtaatac tcctaaacca agcttctttg attttgaagg aaagcaaaaa tgggaagctt 361 ggaaagcact tggtgattca agccccagcc aagcaatgca ggaatatatc gcagtagtta 421 aaaaactaga tccaggttgg aatcctcaga taccagagaa gaaaggaaaa gaagcaaata 481 caggttttgg tgggccagtt attagttctc tatatcatga agaaaccaTC AGGGAAGAAG 541 ACAAAAATAT ATTTGATTAC TGCAGGGAAA ACAACATTGA CCATATAACC AAAGCCATCA 601 AATCGAAAAA TGTGGATGTG AATGTGAAAG ATGAAGAGGG TAGGGCTCTA CTTCACTGGG 661 CCTGTGATCG AGGACATAAG GAACTAGTCA CAGTGTTGCT GCAACATAGA GCTGACATTA 721 ACTGTCAGGA CAATGAAGGC CAAACAGCTC TACATTATGC CTCTGCCTGT GAGTTTCTGG 781 ATATTGTAGA GCTGCTGCTC CAGTCTGGTG CTGACCCCAC TCTCCGAGAC CAGGATGGCT 841 GCCTGCCAGA GGAGGTGACA GGCTGCAAAA CAGTTTCTTT GGTGCTGCAG CGGCACACAA 901 CTGGCAAGGC TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 961 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1021 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT TTGTCGTAGC TTACGCACTC 1081 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t