Construct: ORF TRCN0000477163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006484.1_s317c1
- Derived from:
- ccsbBroadEn_12347
- DNA Barcode:
- GAGTCTTGACCTGTAGGCAGTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHG5 (57449)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57449 | PLEKHG5 | pleckstrin homology and Rho... | NM_001265594.2 | 100% | 100% | |
| 2 | human | 57449 | PLEKHG5 | pleckstrin homology and Rho... | NM_001042664.1 | 92.4% | 91.1% | 2733_2932del;2991_3018del |
| 3 | human | 57449 | PLEKHG5 | pleckstrin homology and Rho... | NM_001042665.1 | 92.4% | 91.1% | 2733_2932del;2991_3018del |
| 4 | human | 57449 | PLEKHG5 | pleckstrin homology and Rho... | NM_020631.5 | 92.4% | 91.1% | 2733_2932del;2991_3018del |
| 5 | human | 57449 | PLEKHG5 | pleckstrin homology and Rho... | NM_001042663.2 | 87.5% | 86.3% | 1_168del;2901_3100del;3159_3186del |
| 6 | human | 57449 | PLEKHG5 | pleckstrin homology and Rho... | NM_001265593.1 | 86.5% | 85.3% | 1_207del;2940_3139del;3198_3225del |
| 7 | human | 57449 | PLEKHG5 | pleckstrin homology and Rho... | NM_198681.3 | 85.8% | 84.6% | 1_231del;2964_3163del;3222_3249del |
| 8 | human | 57449 | PLEKHG5 | pleckstrin homology and Rho... | NM_001265592.1 | 85.7% | 84.5% | 1_237del;2970_3169del;3228_3255del |
| 9 | mouse | 269608 | Plekhg5 | pleckstrin homology domain ... | XM_006538928.1 | 74.4% | 75.9% | (many diffs) |
| 10 | mouse | 269608 | Plekhg5 | pleckstrin homology domain ... | XM_006538927.1 | 73.2% | 74.7% | (many diffs) |
| 11 | mouse | 269608 | Plekhg5 | pleckstrin homology domain ... | XM_006538926.1 | 73.1% | 74.6% | (many diffs) |
| 12 | mouse | 269608 | Plekhg5 | pleckstrin homology domain ... | NM_001285999.1 | 72.2% | 73.7% | (many diffs) |
| 13 | mouse | 269608 | Plekhg5 | pleckstrin homology domain ... | XM_011250266.1 | 71.8% | 73.3% | (many diffs) |
| 14 | mouse | 269608 | Plekhg5 | pleckstrin homology domain ... | XM_006538925.2 | 70.4% | 71.9% | (many diffs) |
| 15 | mouse | 269608 | Plekhg5 | pleckstrin homology domain ... | XM_006538923.3 | 70.3% | 71.7% | (many diffs) |
| 16 | mouse | 269608 | Plekhg5 | pleckstrin homology domain ... | NR_104381.1 | 60.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2856
- ORF length:
- 2790
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ttatgatggg catgtccgct tcgaccttcc cccacaaggc tctgtgctgg 121 cccggaacgt gtccacccgg tcatgcccgc cgcgcaccag ccccgcagtg gacttggagg 181 aggaggagga ggagagctct gtggatggca aaggggaccg gaagagcaca ggcctgaaac 241 tctccaagaa gaaagcaagg aggagacaca cggatgaccc aagcaaggaa tgcttcactc 301 tgaaatttga cctgaatgtg gacattgaga cagagatcgt cccagccatg aagaagaagt 361 cactggggga ggtgctgctg cctgtatttg aaaggaaggg cattgcgctg ggcaaagtgg 421 acatctacct ggaccagtcc aacacacccc tgtccctcac cttcgaggcc tacaggttcg 481 ggggacacta ccttcgtgtc aaagccccag ccaagcctgg agatgagggc aaggtggagc 541 agggcatgaa ggactccaag tccctgagtt tgccgattct gcggccagct gggaccgggc 601 cccccgccct ggagcgtgtg gacgcccaga gccgccggga gagcctggac atcttggccc 661 ctggccgccg ccgcaagaac atgtcggagt tcctggggga ggcgagcatc cccgggcagg 721 agccccccac gccctccagc tgctctctgc ccagcggcag cagtggcagc accaacactg 781 gcgacagctg gaagaaccgg gcggccagtc gcttcagcgg ctttttcagc tccggcccca 841 gcaccagcgc ctttggccgg gaggtagaca agatggagca gctggagggc aagctgcaca 901 cctacagcct cttcgggctg cccaggctgc cccgggggct gcgcttcgac catgactcct 961 gggaggagga gtacgatgaa gacgaggatg aggacaatgc ctgcctgagg ctggaggaca 1021 gctggcggga gctcattgat gggcatgaga agctgacccg gcggcagtgc caccagcagg 1081 aggcggtgtg ggagctgctg cacacggagg cctcctacat caggaaactg cgggtgatca 1141 tcaacctgtt cctgtgctgc ctcctgaacc tgcaagagtc agggctgctg tgtgaggtgg 1201 aggcggagcg cctgttcagc aacatcccgg agatcgcgca gctgcaccgc aggctgtggg 1261 ctagcgtgat ggcgccggtg ctggagaagg cgcggcgcac gcgagcgctg ctacagcccg 1321 gggacttcct caaaggcttc aagatgttcg gctcgctctt caagccctac atccgctact 1381 gcatggagga ggagggctgc atggagtaca tgcgcggcct gctgcgcgac aacgacctct 1441 tccgggccta catcacgtgg gcggagaagc acccacagtg ccagaggctg aagctgagcg 1501 acatgctggc caaaccccac cagcggctca ccaagtaccc gctgctgctc aagtcggtgc 1561 tgaggaagac cgaggagccg cgcgccaagg aggccgtcgt cgccatgatc ggctccgtgg 1621 agcgcttcat ccaccacgtg aacgcgtgca tgcggcagcg gcaggagcgg cagcggctgg 1681 cggccgtggt gagccgcatc gacgcctacg aggtggtgga aagcagcagc gacgaagtgg 1741 acaagctcct gaaggaattt ctgcacctgg acttgacagc gcccatccct ggcgcctccc 1801 cggaggagac gcggcagctg ctgctggagg ggagcctgag gatgaaggag gggaaggaca 1861 gcaagatgga tgtgtactgc ttcctcttca cggatctgct gttggtgacc aaagcagtga 1921 agaaggcaga gaggaccagg gtcatcaggc cacccctgct cgtggacaag attgtgtgcc 1981 gggagctacg ggaccctggg tccttcctcc ttatctacct gaatgagttt cacagtgctg 2041 taggggccta cacgttccag gccagtggcc aggccttgtg ccgtggctgg gtggacacca 2101 tttacaatgc ccagaaccag ctgcaacagc tgcgtgcaca ggagccccca ggcagtcagc 2161 agcccctgca gagcctggaa gaggaggagg atgagcagga ggaggaagag gaggaggagg 2221 aggaggagga ggaaggcgag gacagtggca cttcagctgc cagctcccct accatcatgc 2281 ggaaaagcag cggcagcccc gactctcagc actgtgcctc agatggctcc acggagaccc 2341 tggccatggt tgtggtagag cctggggaca cgctgtcctc ccccgagttc gacagcggtc 2401 ctttcagctc ccagtctgat gagacctctc tcagcaccac tgcctcatct gccacgccca 2461 ccagtgagct gctgcccctg ggtccggtgg acggccgctc ctgctccatg gactctgccT 2521 ACGGCACCCT CTCCCCAACC TCCTTACAAG ACTTTGTGGC CCCAGGCCCA ATGGCAGAGC 2581 TAGTGCCTCG GGCCCCAGAG TCCCCACGAG TTCCTTCCCC TCCACCCTCG CCCCGTCTCC 2641 GCCGCCGCAC CCCTGTCCAG CTGTTGAGCT GCCCGCCCCA CCTGCTCAAG TCTAAGTCCG 2701 AGGCCAGCCT CCTCCAGCTG CTGGCAGGGG CTGGCACCCA TGGGACACCC TCTGCCCCCA 2761 GCCGCAGCCT GTCAGAGCTC TGCCTGGCTG TTCCAGCCCC AGCACAGGAA GCTGACCCTG 2821 GCCCAGCTCT ACCGAATCAG GACCACCCTG CTGCTTACCC AACTTTCTTG TACAAAGTGG 2881 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2941 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3001 AGAGTCTTGA CCTGTAGGCA GTGACACGCG TTAAGTCgac aatcaacctc tggattacaa 3061 aatttgtgaa agatt