Construct: ORF TRCN0000477173
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008198.1_s317c1
- Derived from:
- ccsbBroadEn_08713
- DNA Barcode:
- GCAAGACGTCATATGTCCCCACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PELI2 (57161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477173
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57161 | PELI2 | pellino E3 ubiquitin protei... | NM_021255.3 | 100% | 100% | |
| 2 | human | 57161 | PELI2 | pellino E3 ubiquitin protei... | XM_017021478.1 | 96.2% | 94.7% | (many diffs) |
| 3 | human | 57161 | PELI2 | pellino E3 ubiquitin protei... | XM_006720211.3 | 76.1% | 76.1% | 0_1ins300 |
| 4 | human | 57161 | PELI2 | pellino E3 ubiquitin protei... | XM_011536990.2 | 76.1% | 76.1% | 0_1ins300 |
| 5 | human | 57161 | PELI2 | pellino E3 ubiquitin protei... | XM_011536992.3 | 76.1% | 76.1% | 0_1ins300 |
| 6 | human | 57161 | PELI2 | pellino E3 ubiquitin protei... | XM_017021479.1 | 76.1% | 76.1% | 0_1ins300 |
| 7 | human | 57161 | PELI2 | pellino E3 ubiquitin protei... | XM_005267890.5 | 57.4% | 56.1% | (many diffs) |
| 8 | mouse | 93834 | Peli2 | pellino 2 | NM_033602.2 | 86.9% | 95.2% | (many diffs) |
| 9 | mouse | 93834 | Peli2 | pellino 2 | XM_006519740.3 | 74.5% | 82.3% | (many diffs) |
| 10 | mouse | 93834 | Peli2 | pellino 2 | XM_006519741.2 | 66.9% | 74.2% | (many diffs) |
| 11 | mouse | 93834 | Peli2 | pellino 2 | XM_006519742.3 | 53.7% | 47.3% | (many diffs) |
| 12 | mouse | 93834 | Peli2 | pellino 2 | XM_011245231.2 | 53.6% | 49.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1326
- ORF length:
- 1260
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ttcccctggc caggaggaac actgcgcccc caataaggag ccagtgaaat 121 acggggagct ggtggtgctc gggtacaatg gtgctttacc caatggagat agaggacgga 181 ggaaaagtag atttgccctc tacaagcggc ccaaggcaaa tggtgtcaaa cccagcaccg 241 tccatgtgat atccacgccc caggcatcca aggctatcag ctgcaaaggt caacacagta 301 tatcctacac tttgtcaagg aatcagactg tggtggtgga gtacacacat gataaggata 361 cggatatgtt tcaggtgggc agatcaacag aaagccctat cgacttcgtt gtcacagaca 421 cgatttctgg cagccagaac acggacgaag cccagatcac acagagcacc atatccaggt 481 tcgcctgcag gatcgtgtgc gacaggaatg aaccttacac agcacggata ttcgccgccg 541 gatttgactc ttccaaaaac atatttcttg gagaaaaggc agcaaagtgg aaaaaccccg 601 acggccacat ggatgggctc actactaatg gcgtcctggt gatgcatcca cgagggggct 661 tcaccgagga gtcccagccc ggggtctggc gcgagatctc tgtctgtgga gatgtgtaca 721 ccttgcgaga aaccaggtcg gcccagcaac gaggaaagct ggtggaaagt gagaccaacg 781 tcctgcagga cggctccctc attgacctgt gtggggccac tctcctctgg agaacagcag 841 atgggctttt tcatactcca actcagaagc acatagaagc cctccggcag gagattaacg 901 ccgcccggcc tcagtgtcct gtggggctca acaccctggc cttccccagc atcaacagga 961 aagaggtggt ggaggagaag cagccctggg catatcTCAG TTGTGGCCAC GTGCACGGGT 1021 ACCACAACTG GGGCCATCGG AGTGACACGG AGGCCAACGA GAGGGAGTGT CCCATGTGCA 1081 GGACTGTGGG CCCCTATGTG CCTCTCTGGC TTGGCTGTGA GGCAGGATTT TATGTAGACG 1141 CAGGACCGCC AACTCATGCT TTCACTCCCT GTGGACACGT GTGCTCGGAG AAGTCTGCAA 1201 AATACTGGTC TCAGATCCCG TTGCCTCATG GAACTCATGC ATTTCACGCT GCTTGCCCTT 1261 TCTGTGCTAC ACAGCTGGTT GGGGAGCAAA ACTGCATCAA ATTAATTTTC CAAGGTCCAA 1321 TTGACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1381 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1441 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCAAGACGT CATATGTCCC CACTCACGCG 1501 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt