Construct: ORF TRCN0000477175
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004216.1_s317c1
- Derived from:
- ccsbBroadEn_09476
- DNA Barcode:
- TAGTTTTCAAACACCGATGGCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEGS2 (123099)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477175
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 123099 | DEGS2 | delta 4-desaturase, sphingo... | NM_206918.3 | 99.7% | 99.6% | 169G>A;465A>G |
| 2 | human | 123099 | DEGS2 | delta 4-desaturase, sphingo... | XM_006720043.3 | 88.6% | 88.5% | 0_1ins108;61G>A;357A>G |
| 3 | mouse | 70059 | Degs2 | delta(4)-desaturase, sphing... | NM_027299.5 | 82.5% | 86% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1038
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaacagc gcgagccgca gcgacttcga gtgggtctac accgaccagc 121 cgcacacgca gcggcgcaag gagatactgg ccaagtaccc ggccatcaag gccctgatgc 181 ggccagaccc gcgcctcaag tgggcggtgc tggtgctggt gctggtgcag atgctgacct 241 gctggctggt gcgcgggctg gcctggcgct ggctgctgtt ctgggcctac gcctttggtg 301 gctgcgtgaa ccactcgctg acgctggcca tccacgacat ctcgcacaac gcggccttcg 361 gcacgggccg tgcggcacgc aaccgctggc tggccgtgtt cgccaacctg cccgtgggtg 421 tgccctacgc cgcctccttc aagaagtacc acgtggacca ccaccgctac ctgggcggcg 481 acgggctgga cgtggacgtg cccacgcgtc tggagggctg gttcttctgc acgcccgccc 541 gcaagctgct ctggctggtg ctgcagccct tcttctactc actacggccg ctctgcgtcc 601 accccaaggc cgtgacccgc atggaggtgc tcaacacgct ggtgcagctg gcggccgacc 661 tggccatctt tgccctttgg GGGCTCAAGC CCGTGGTCTA CCTGCTGGCC AGCTCCTTCC 721 TGGGCCTGGG CCTGCACCCC ATCTCGGGCC ACTTCGTGGC CGAGCACTAC ATGTTCCTCA 781 AGGGCCACGA GACCTACTCC TACTATGGGC CTCTCAACTG GATCACCTTC AATGTGGGCT 841 ACCACGTGGA GCACCACGAC TTCCCCAGCA TCCCGGGCTA CAACCTGCCG CTGGTGCGGA 901 AGATCGCGCC CGAGTACTAC GACCACCTGC CGCAGCACCA CTCCTGGGTG AAGGTGCTCT 961 GGGATTTTGT GTTTGAGGAC TCCCTGGGGC CCTATGCCAG GGTGAAGCGG GTGTACAGGC 1021 TGGCAAAAGA TGGTCTGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1081 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1141 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATAGTTTT CAAACACCGA 1201 TGGCATAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt