Construct: ORF TRCN0000477224
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016689.1_s317c1
- Derived from:
- ccsbBroadEn_02755
- DNA Barcode:
- ACTGCCACTGATAAACTACCGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R13B (23368)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477224
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | NM_015316.3 | 100% | 100% | |
| 2 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XM_017021116.1 | 96.8% | 96.8% | 1151_1255del |
| 3 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XM_017021117.1 | 96.6% | 96.5% | 0_1insATGATGCCG;1142_1246del |
| 4 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XM_005267487.5 | 94% | 93.8% | (many diffs) |
| 5 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XM_011536593.3 | 91.3% | 91% | (many diffs) |
| 6 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XM_017021119.1 | 74% | 71.5% | (many diffs) |
| 7 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XR_001750206.2 | 64.7% | (many diffs) | |
| 8 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XR_001750204.2 | 60.6% | (many diffs) | |
| 9 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XR_001750205.2 | 59.5% | (many diffs) | |
| 10 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XR_245676.4 | 54.1% | (many diffs) | |
| 11 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XR_943413.3 | 53.2% | (many diffs) | |
| 12 | mouse | 21981 | Ppp1r13b | protein phosphatase 1, regu... | NM_011625.1 | 86% | 91.8% | (many diffs) |
| 13 | mouse | 21981 | Ppp1r13b | protein phosphatase 1, regu... | XM_006515796.3 | 83.3% | 88.8% | (many diffs) |
| 14 | mouse | 21981 | Ppp1r13b | protein phosphatase 1, regu... | XM_006515797.3 | 83% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3339
- ORF length:
- 3270
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatgccgatg atattaactg ttttcttgag caacaatgaa cagattttaa 121 cagaagttcc tataacaccg gaaacaacct gtcgagatgt tgtagaattt tgcaaggaac 181 ctggagaagg cagctgccat ttagctgaag tgtggagggg aaatgaacgt cccataccct 241 ttgatcatat gatgtacgaa catcttcaga aatggggtcc acggagggaa gaagtgaaat 301 ttttccttcg acacgaggac tccccaactg agaacagtga acaaggtggc cgtcagaccc 361 aagagcaacg aactcagaga aatgtaataa atgtacctgg agaaaaacgt actgaaaatg 421 gggttgggaa tccacgtgtt gaacttaccc tctcagagct ccaagatatg gcagctaggc 481 aacagcagca gattgaaaat cagcagcaga tgttggttgc caaggaacag cgtttacatt 541 ttctaaagca acaggagcgc cgtcagcagc agtctatttc tgaaaatgaa aagcttcaga 601 aattgaaaga acgagttgaa gcccaggaga acaagctgaa gaaaattcgt gcaatgagag 661 gacaagtcga ctacagcaaa atcatgaacg gcaatctgtc tgctgaaata gaaaggttca 721 gtgccatgtt ccaggaaaag aagcaggaag tacagactgc aattttaagg gttgatcagc 781 ttagtcagca attggaagat ttaaagaaag gaaaactgaa tgggttccag tcttacaatg 841 gcaaattgac gggaccagcg gcggtggagt taaaaagact gtaccaagaa ctacagattc 901 gtaaccaact taaccaggaa caaaattcaa aacttcagca gcagaaggaa ctcttaaata 961 agcgcaacat ggaggtggcc atgatggaca agcgaatcag tgaactgcgt gaacgtctct 1021 atgggaaaaa aattcagctg aaccgtgtga atggcacgtc atcaccacag tcccctctga 1081 gcacatcggg cagggtcgct gctgtggggc cttatatcca ggttcccagt gccggaagct 1141 ttcctgtgct gggggaccct ataaagcccc agtctctcag tattgcctca aatgctgctc 1201 atggaagatc caaatccgct aatgatggaa actggccaac attaaaacag aattctagct 1261 cttccgtgaa accagtgcag gtggccggtg cagactggaa ggatccgagc gtggaggggt 1321 ctgtcaagca gggcactgtc tccagccagc ctgtgccctt ctcagcactg ggacccacgg 1381 agaagccggg catcgagatt ggtaaagtgc cacctcccat cccgggtgta ggcaagcagc 1441 tgcctccaag ctatgggaca tacccaagtc ctacacctct gggtcctggg tcgacaagct 1501 ccctggaaag gaggaaggaa ggcagcttgc ccaggcccag tgcaggcctg ccaagtcgac 1561 agaggcccac cctgctgccc gccacaggca gcacccccca gccaggctcc tcacaacaga 1621 ttcagcagag gatttccgta ccgccaagtc ccacgtaccc gccagcggga ccacctgcat 1681 ttccagctgg ggacagcaag cctgaactcc cactgacagt ggccattagg cctttcctgg 1741 ctgataaagg gtcaaggcca cagtctccca ggaaaggacc ccagacagtg aattcaagtt 1801 ccatatactc catgtacctc cagcaagcca caccacctaa gaattaccag ccggcagcac 1861 acagcgcctt aaataagtca gttaaagcag tgtatggtaa gcccgtttta ccttcgggtt 1921 caacctctcc atcgccgctg ccgtttcttc acgggtcact gtccacgggc acaccacagc 1981 ctcagccacc ttcagaaagt actgagaaag agcctgagca ggatggcccc gccgcccccg 2041 cagatggcag caccgtggag agcctgccac ggccactcag ccccaccaag ctcacgccca 2101 tcgtgcattc gccactgcgc taccagagtg atgcagacct ggaggccctc cgcaggaagc 2161 tggccaacgc gccccggccc ctgaaaaagc gcagctccat cacagagccc gagggccccg 2221 gcgggcccaa catccagaag ctgctgtacc agcgcttcaa caccctggcc ggtggcatgg 2281 agggcacccc tttctaccag cccagcccct cccaggactt catgggcacc ttggccgatg 2341 tggacaatgg aaacaccaat gccaatggaa acctggaaga gctcccccct gcccagccca 2401 cagccccact ccccgctgag cctgccccgt catcagatgc caatgataat gagttacctt 2461 cccccgaacc agaggagctc atctgtcccc aaaccaccca ccaaactgcc gagccggcag 2521 aggacaataa caacaacgtg gccacggtcc ccaccacgga gcagatcccg agtcctgtgg 2581 ctgaggcccc atctccaggg gaagagcagg tccctccagc acctcttccc cctgccagcc 2641 accctcctgc cacctccacg aacaagcgga ccaacttgaa gaagcccaac tcggagcgga 2701 cggggcacgg gctgagagtc cggtttaacc ccctggcact gctcctagac gcgtctctgg 2761 aaggagagtt cgatctggtg cagaggatca tctatgaggt ggaagatccc agcaagccca 2821 acgacgaagg gatcacccca ctgcacaacg ccgtctgcgc cggccaccat cacatcgtga 2881 agttccTGCT GGATTTTGGT GTCAACGTGA ATGCTGCTGA TAGTGATGGA TGGACGCCGC 2941 TGCACTGCGC TGCCTCTTGT AACAGCGTTC ACCTCTGCAA ACAGCTGGTG GAGAGTGGTG 3001 CCGCCATTTT TGCCTCAACC ATAAGCGACA TTGAAACTGC TGCAGACAAG TGTGAGGAGA 3061 TGGAGGAAGG CTACATCCAG TGCTCCCAGT TTCTATATGG GGTGCAGGAA AAGCTGGGTG 3121 TGATGAACAA AGGTGTGGCG TATGCTCTGT GGGACTACGA GGCCCAGAAC AGTGACGAGC 3181 TGTCCTTCCA CGAAGGGGAC GCCCTCACCA TCCTGAGGCG CAAGGACGAA AGCGAGACTG 3241 AGTGGTGGTG GGCTCGCCTT GGAGACCGGG AGGGCTATGT GCCCAAAAAC CTGCTGGGGC 3301 TGTATCCACG GATCAAACCC CGACAGCGAA CACTCGCCTT GCCAACTTTC TTGTACAAAG 3361 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3421 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3481 ACGAACTGCC ACTGATAAAC TACCGCGTAC GCGTTAAGTC gacaatcaac ctctggatta 3541 caaaatttgt gaaagatt