Construct: ORF TRCN0000477224

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016689.1_s317c1
Derived from:
ccsbBroadEn_02755
DNA Barcode:
ACTGCCACTGATAAACTACCGCGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPP1R13B (23368)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477224
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23368 PPP1R13B protein phosphatase 1 regul... NM_015316.3 100% 100%
2 human 23368 PPP1R13B protein phosphatase 1 regul... XM_017021116.1 96.8% 96.8% 1151_1255del
3 human 23368 PPP1R13B protein phosphatase 1 regul... XM_017021117.1 96.6% 96.5% 0_1insATGATGCCG;1142_1246del
4 human 23368 PPP1R13B protein phosphatase 1 regul... XM_005267487.5 94% 93.8% (many diffs)
5 human 23368 PPP1R13B protein phosphatase 1 regul... XM_011536593.3 91.3% 91% (many diffs)
6 human 23368 PPP1R13B protein phosphatase 1 regul... XM_017021119.1 74% 71.5% (many diffs)
7 human 23368 PPP1R13B protein phosphatase 1 regul... XR_001750206.2 64.7% (many diffs)
8 human 23368 PPP1R13B protein phosphatase 1 regul... XR_001750204.2 60.6% (many diffs)
9 human 23368 PPP1R13B protein phosphatase 1 regul... XR_001750205.2 59.5% (many diffs)
10 human 23368 PPP1R13B protein phosphatase 1 regul... XR_245676.4 54.1% (many diffs)
11 human 23368 PPP1R13B protein phosphatase 1 regul... XR_943413.3 53.2% (many diffs)
12 mouse 21981 Ppp1r13b protein phosphatase 1, regu... NM_011625.1 86% 91.8% (many diffs)
13 mouse 21981 Ppp1r13b protein phosphatase 1, regu... XM_006515796.3 83.3% 88.8% (many diffs)
14 mouse 21981 Ppp1r13b protein phosphatase 1, regu... XM_006515797.3 83% 88.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3339
ORF length:
3270
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gatgccgatg atattaactg ttttcttgag caacaatgaa cagattttaa
121cagaagttcc tataacaccg gaaacaacct gtcgagatgt tgtagaattt tgcaaggaac
181ctggagaagg cagctgccat ttagctgaag tgtggagggg aaatgaacgt cccataccct
241ttgatcatat gatgtacgaa catcttcaga aatggggtcc acggagggaa gaagtgaaat
301ttttccttcg acacgaggac tccccaactg agaacagtga acaaggtggc cgtcagaccc
361aagagcaacg aactcagaga aatgtaataa atgtacctgg agaaaaacgt actgaaaatg
421gggttgggaa tccacgtgtt gaacttaccc tctcagagct ccaagatatg gcagctaggc
481aacagcagca gattgaaaat cagcagcaga tgttggttgc caaggaacag cgtttacatt
541ttctaaagca acaggagcgc cgtcagcagc agtctatttc tgaaaatgaa aagcttcaga
601aattgaaaga acgagttgaa gcccaggaga acaagctgaa gaaaattcgt gcaatgagag
661gacaagtcga ctacagcaaa atcatgaacg gcaatctgtc tgctgaaata gaaaggttca
721gtgccatgtt ccaggaaaag aagcaggaag tacagactgc aattttaagg gttgatcagc
781ttagtcagca attggaagat ttaaagaaag gaaaactgaa tgggttccag tcttacaatg
841gcaaattgac gggaccagcg gcggtggagt taaaaagact gtaccaagaa ctacagattc
901gtaaccaact taaccaggaa caaaattcaa aacttcagca gcagaaggaa ctcttaaata
961agcgcaacat ggaggtggcc atgatggaca agcgaatcag tgaactgcgt gaacgtctct
1021atgggaaaaa aattcagctg aaccgtgtga atggcacgtc atcaccacag tcccctctga
1081gcacatcggg cagggtcgct gctgtggggc cttatatcca ggttcccagt gccggaagct
1141ttcctgtgct gggggaccct ataaagcccc agtctctcag tattgcctca aatgctgctc
1201atggaagatc caaatccgct aatgatggaa actggccaac attaaaacag aattctagct
1261cttccgtgaa accagtgcag gtggccggtg cagactggaa ggatccgagc gtggaggggt
1321ctgtcaagca gggcactgtc tccagccagc ctgtgccctt ctcagcactg ggacccacgg
1381agaagccggg catcgagatt ggtaaagtgc cacctcccat cccgggtgta ggcaagcagc
1441tgcctccaag ctatgggaca tacccaagtc ctacacctct gggtcctggg tcgacaagct
1501ccctggaaag gaggaaggaa ggcagcttgc ccaggcccag tgcaggcctg ccaagtcgac
1561agaggcccac cctgctgccc gccacaggca gcacccccca gccaggctcc tcacaacaga
1621ttcagcagag gatttccgta ccgccaagtc ccacgtaccc gccagcggga ccacctgcat
1681ttccagctgg ggacagcaag cctgaactcc cactgacagt ggccattagg cctttcctgg
1741ctgataaagg gtcaaggcca cagtctccca ggaaaggacc ccagacagtg aattcaagtt
1801ccatatactc catgtacctc cagcaagcca caccacctaa gaattaccag ccggcagcac
1861acagcgcctt aaataagtca gttaaagcag tgtatggtaa gcccgtttta ccttcgggtt
1921caacctctcc atcgccgctg ccgtttcttc acgggtcact gtccacgggc acaccacagc
1981ctcagccacc ttcagaaagt actgagaaag agcctgagca ggatggcccc gccgcccccg
2041cagatggcag caccgtggag agcctgccac ggccactcag ccccaccaag ctcacgccca
2101tcgtgcattc gccactgcgc taccagagtg atgcagacct ggaggccctc cgcaggaagc
2161tggccaacgc gccccggccc ctgaaaaagc gcagctccat cacagagccc gagggccccg
2221gcgggcccaa catccagaag ctgctgtacc agcgcttcaa caccctggcc ggtggcatgg
2281agggcacccc tttctaccag cccagcccct cccaggactt catgggcacc ttggccgatg
2341tggacaatgg aaacaccaat gccaatggaa acctggaaga gctcccccct gcccagccca
2401cagccccact ccccgctgag cctgccccgt catcagatgc caatgataat gagttacctt
2461cccccgaacc agaggagctc atctgtcccc aaaccaccca ccaaactgcc gagccggcag
2521aggacaataa caacaacgtg gccacggtcc ccaccacgga gcagatcccg agtcctgtgg
2581ctgaggcccc atctccaggg gaagagcagg tccctccagc acctcttccc cctgccagcc
2641accctcctgc cacctccacg aacaagcgga ccaacttgaa gaagcccaac tcggagcgga
2701cggggcacgg gctgagagtc cggtttaacc ccctggcact gctcctagac gcgtctctgg
2761aaggagagtt cgatctggtg cagaggatca tctatgaggt ggaagatccc agcaagccca
2821acgacgaagg gatcacccca ctgcacaacg ccgtctgcgc cggccaccat cacatcgtga
2881agttccTGCT GGATTTTGGT GTCAACGTGA ATGCTGCTGA TAGTGATGGA TGGACGCCGC
2941TGCACTGCGC TGCCTCTTGT AACAGCGTTC ACCTCTGCAA ACAGCTGGTG GAGAGTGGTG
3001CCGCCATTTT TGCCTCAACC ATAAGCGACA TTGAAACTGC TGCAGACAAG TGTGAGGAGA
3061TGGAGGAAGG CTACATCCAG TGCTCCCAGT TTCTATATGG GGTGCAGGAA AAGCTGGGTG
3121TGATGAACAA AGGTGTGGCG TATGCTCTGT GGGACTACGA GGCCCAGAAC AGTGACGAGC
3181TGTCCTTCCA CGAAGGGGAC GCCCTCACCA TCCTGAGGCG CAAGGACGAA AGCGAGACTG
3241AGTGGTGGTG GGCTCGCCTT GGAGACCGGG AGGGCTATGT GCCCAAAAAC CTGCTGGGGC
3301TGTATCCACG GATCAAACCC CGACAGCGAA CACTCGCCTT GCCAACTTTC TTGTACAAAG
3361TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
3421GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
3481ACGAACTGCC ACTGATAAAC TACCGCGTAC GCGTTAAGTC gacaatcaac ctctggatta
3541caaaatttgt gaaagatt

Download FASTA (ORF) (Full)