Construct: ORF TRCN0000477307
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012039.1_s317c1
- Derived from:
- ccsbBroadEn_06327
- DNA Barcode:
- TTAGTATTTATGGTTTTCTTGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRM8 (2918)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477307
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_000845.3 | 99.9% | 99.7% | 17A>G;2486C>T |
| 2 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371083.1 | 99.9% | 99.7% | 17A>G;2486C>T |
| 3 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371084.1 | 99.9% | 99.7% | 17A>G;2486C>T |
| 4 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001127323.1 | 98.5% | 98.2% | (many diffs) |
| 5 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371085.1 | 98.5% | 98.2% | (many diffs) |
| 6 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371086.1 | 97.7% | 96.2% | (many diffs) |
| 7 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516094.1 | 80.5% | 79.7% | (many diffs) |
| 8 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371087.1 | 77.3% | 77.3% | 0_1ins615;1871C>T |
| 9 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012075.1 | 77.3% | 77.3% | 0_1ins615;1871C>T |
| 10 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012076.1 | 77.3% | 77.3% | 0_1ins615;1871C>T |
| 11 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012077.1 | 77.3% | 77.3% | 0_1ins615;1871C>T |
| 12 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012078.1 | 77.3% | 77.3% | 0_1ins615;1871C>T |
| 13 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012079.1 | 77.3% | 77.3% | 0_1ins615;1871C>T |
| 14 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516095.2 | 77.2% | 76.8% | (many diffs) |
| 15 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516101.2 | 76% | 75.7% | (many diffs) |
| 16 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516102.2 | 76% | 75.7% | (many diffs) |
| 17 | human | 2918 | GRM8 | glutamate metabotropic rece... | NR_028041.1 | 74.7% | (many diffs) | |
| 18 | human | 2918 | GRM8 | glutamate metabotropic rece... | NR_163850.1 | 69.7% | (many diffs) | |
| 19 | human | 2918 | GRM8 | glutamate metabotropic rece... | NR_163849.1 | 68.4% | (many diffs) | |
| 20 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516103.2 | 67.9% | 67.6% | (many diffs) |
| 21 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_017012080.1 | 67.9% | 67.6% | (many diffs) |
| 22 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_024446736.1 | 67.9% | 67.6% | (many diffs) |
| 23 | human | 2918 | GRM8 | glutamate metabotropic rece... | XM_011516108.2 | 58.6% | 56.5% | (many diffs) |
| 24 | human | 2918 | GRM8 | glutamate metabotropic rece... | NM_001371088.1 | 50% | 49.6% | (many diffs) |
| 25 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | NM_001311072.1 | 91.8% | 98.2% | (many diffs) |
| 26 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_017321397.1 | 91.8% | 98.2% | (many diffs) |
| 27 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | NM_008174.2 | 90.8% | 96.6% | (many diffs) |
| 28 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504987.3 | 90.8% | 96.6% | (many diffs) |
| 29 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504989.1 | 90.8% | 96.6% | (many diffs) |
| 30 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504990.3 | 90.8% | 96.6% | (many diffs) |
| 31 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_006504991.3 | 90.8% | 96.6% | (many diffs) |
| 32 | mouse | 14823 | Grm8 | glutamate receptor, metabot... | XM_017321398.1 | 62.8% | 66.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2793
- ORF length:
- 2724
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtatgcgag ggaaggcgat cagcctcttg cccttgtttc ttcctcttga 121 ccgccaagtt ctactggatc ctcacaatga tgcaaagaac tcacagccag gagtatgccc 181 attccatacg ggtggatggg gacattattt tggggggtct cttccctgtc cacgcaaagg 241 gagagagagg ggtgccttgt ggggagctga agaaggaaaa ggggattcac agactggagg 301 ccatgcttta tgcaattgac cagattaaca aggaccctga tctcctttcc aacatcactc 361 tgggtgtccg catcctcgac acgtgctcta gggacaccta tgctttggag cagtctctaa 421 cattcgtgca ggcattaata gagaaagatg cttcggatgt gaagtgtgct aatggagatc 481 cacccatttt caccaagccc gacaagattt ctggcgtcat aggtgctgca gcaagctccg 541 tgtccatcat ggttgctaac attttaagac tttttaagat acctcaaatc agctatgcat 601 ccacagcccc agagctaagt gataacacca ggtatgactt tttctctcga gtggttccgc 661 ctgactccta ccaagcccaa gccatggtgg acatcgtgac agcactggga tggaattatg 721 tttcgacact ggcttctgag gggaactatg gtgagagcgg tgtggaggcc ttcacccaga 781 tctcgaggga gattggtggt gtttgcattg ctcagtcaca gaaaatccca cgtgaaccaa 841 gacctggaga atttgaaaaa attatcaaac gcctgctaga aacacctaat gctcgagcag 901 tgattatgtt tgccaatgag gatgacatca ggaggatatt ggaagcagca aaaaaactaa 961 accaaagtgg gcattttctc tggattggct cagatagttg gggatccaaa atagcacctg 1021 tctatcagca agaggagatt gcagaagggg ctgtgacaat tttgcccaaa cgagcatcaa 1081 ttgatggatt tgatcgatac tttagaagcc gaactcttgc caataatcga agaaatgtgt 1141 ggtttgcaga attctgggag gagaattttg gctgcaagtt aggatcacat gggaaaagga 1201 acagtcatat aaagaaatgc acagggctgg agcgaattgc tcgggattca tcttatgaac 1261 aggaaggaaa ggtccaattt gtaattgatg ctgtatattc catggcttac gccctgcaca 1321 atatgcacaa agatctctgc cctggataca ttggcctttg tccacgaatg agtaccattg 1381 atgggaaaga gctacttggt tatattcggg ctgtaaattt taatggcagt gctggcactc 1441 ctgtcacttt taatgaaaac ggagatgctc ctggacgtta tgatatcttc cagtatcaaa 1501 taaccaacaa aagcacagag tacaaagtca tcggccactg gaccaatcag cttcatctaa 1561 aagtggaaga catgcagtgg gctcatagag aacatactca cccggcgtct gtctgcagcc 1621 tgccgtgtaa gccaggggag aggaagaaaa cggtgaaagg ggtcccttgc tgctggcact 1681 gtgaacgctg tgaaggttac aactaccagg tggatgagct gtcctgtgaa ctttgccctc 1741 tggatcagag acccaacatg aaccgcacag gctgccagct tatccccatc atcaaattgg 1801 agtggcattc tccctgggct gtggtgcctg tgtttgttgc aatattggga atcatcgcca 1861 ccacctttgt gatcgtgacc tttgtccgct ataatgacac acctatcgtg agggcttcag 1921 gacgcgaact tagttacgtg ctcctaacgg ggatttttct ctgttattca atcacgtttt 1981 taatgattgc agcaccagat acaatcatat gctccttccg acgggtcttc ctaggacttg 2041 gcatgtgttt cagctatgca gcccttctga ccaaaacaaa ccgtatccac cgaatatttg 2101 agcaggggaa gaaatctgtc acagcgccca agttcattag tccagcatct cagctggtga 2161 tcaccttcag cctcatctcc gtccagctcc ttggagtgtt tgtctggttt gttgtggatc 2221 ccccccacat catcattgac tatggagagc agcggacact agatccagag aaggccaggg 2281 gagtgctcaa gtgtgacatt tctgatctct cactcatttg ttcacttgga tacagtatcc 2341 TCTTGATGGT CACTTGTACT GTTTATGCCA TTAAAACGAG AGGTGTCCCA GAGACTTTCA 2401 ATGAAGCCAA ACCTATTGGA TTTACCATGT ATACCACCTG CATCATTTGG TTAGCTTTCA 2461 TCCCCATCTT TTTTGGTACA GCCCAGTCAG CAGAAAAGAT GTACATCCAG ACAACAACAC 2521 TTACTGTCTC CATGAGTTTA AGTGCTTCAG TATTTCTGGG CATGCTCTAT ATGCCCAAGG 2581 TTTATATTAT AATTTTTCAT CCAGAACAGA ATGTTCAAAA ACGCAAGAGG AGCTTCAAGG 2641 CTGTGGTGAC AGCTGCCACC ATGCAAAGCA AACTGATCCA AAAAGGAAAT GACAGACCAA 2701 ATGGCGAGGT GAAAAGTGAA CTCTGTGAGA GTCTTGAAAC CAACACTTCC TCTACCAAGA 2761 CAACATATAT CAGTTACAGC AATCATTCAA TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2821 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2881 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 2941 AGTATTTATG GTTTTCTTGA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 3001 ttgtgaaaga tt