Construct: ORF TRCN0000477307

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012039.1_s317c1
Derived from:
ccsbBroadEn_06327
DNA Barcode:
TTAGTATTTATGGTTTTCTTGATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRM8 (2918)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477307
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2918 GRM8 glutamate metabotropic rece... NM_000845.3 99.9% 99.7% 17A>G;2486C>T
2 human 2918 GRM8 glutamate metabotropic rece... NM_001371083.1 99.9% 99.7% 17A>G;2486C>T
3 human 2918 GRM8 glutamate metabotropic rece... NM_001371084.1 99.9% 99.7% 17A>G;2486C>T
4 human 2918 GRM8 glutamate metabotropic rece... NM_001127323.1 98.5% 98.2% (many diffs)
5 human 2918 GRM8 glutamate metabotropic rece... NM_001371085.1 98.5% 98.2% (many diffs)
6 human 2918 GRM8 glutamate metabotropic rece... NM_001371086.1 97.7% 96.2% (many diffs)
7 human 2918 GRM8 glutamate metabotropic rece... XM_011516094.1 80.5% 79.7% (many diffs)
8 human 2918 GRM8 glutamate metabotropic rece... NM_001371087.1 77.3% 77.3% 0_1ins615;1871C>T
9 human 2918 GRM8 glutamate metabotropic rece... XM_017012075.1 77.3% 77.3% 0_1ins615;1871C>T
10 human 2918 GRM8 glutamate metabotropic rece... XM_017012076.1 77.3% 77.3% 0_1ins615;1871C>T
11 human 2918 GRM8 glutamate metabotropic rece... XM_017012077.1 77.3% 77.3% 0_1ins615;1871C>T
12 human 2918 GRM8 glutamate metabotropic rece... XM_017012078.1 77.3% 77.3% 0_1ins615;1871C>T
13 human 2918 GRM8 glutamate metabotropic rece... XM_017012079.1 77.3% 77.3% 0_1ins615;1871C>T
14 human 2918 GRM8 glutamate metabotropic rece... XM_011516095.2 77.2% 76.8% (many diffs)
15 human 2918 GRM8 glutamate metabotropic rece... XM_011516101.2 76% 75.7% (many diffs)
16 human 2918 GRM8 glutamate metabotropic rece... XM_011516102.2 76% 75.7% (many diffs)
17 human 2918 GRM8 glutamate metabotropic rece... NR_028041.1 74.7% (many diffs)
18 human 2918 GRM8 glutamate metabotropic rece... NR_163850.1 69.7% (many diffs)
19 human 2918 GRM8 glutamate metabotropic rece... NR_163849.1 68.4% (many diffs)
20 human 2918 GRM8 glutamate metabotropic rece... XM_011516103.2 67.9% 67.6% (many diffs)
21 human 2918 GRM8 glutamate metabotropic rece... XM_017012080.1 67.9% 67.6% (many diffs)
22 human 2918 GRM8 glutamate metabotropic rece... XM_024446736.1 67.9% 67.6% (many diffs)
23 human 2918 GRM8 glutamate metabotropic rece... XM_011516108.2 58.6% 56.5% (many diffs)
24 human 2918 GRM8 glutamate metabotropic rece... NM_001371088.1 50% 49.6% (many diffs)
25 mouse 14823 Grm8 glutamate receptor, metabot... NM_001311072.1 91.8% 98.2% (many diffs)
26 mouse 14823 Grm8 glutamate receptor, metabot... XM_017321397.1 91.8% 98.2% (many diffs)
27 mouse 14823 Grm8 glutamate receptor, metabot... NM_008174.2 90.8% 96.6% (many diffs)
28 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504987.3 90.8% 96.6% (many diffs)
29 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504989.1 90.8% 96.6% (many diffs)
30 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504990.3 90.8% 96.6% (many diffs)
31 mouse 14823 Grm8 glutamate receptor, metabot... XM_006504991.3 90.8% 96.6% (many diffs)
32 mouse 14823 Grm8 glutamate receptor, metabot... XM_017321398.1 62.8% 66.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2793
ORF length:
2724
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggtatgcgag ggaaggcgat cagcctcttg cccttgtttc ttcctcttga
121ccgccaagtt ctactggatc ctcacaatga tgcaaagaac tcacagccag gagtatgccc
181attccatacg ggtggatggg gacattattt tggggggtct cttccctgtc cacgcaaagg
241gagagagagg ggtgccttgt ggggagctga agaaggaaaa ggggattcac agactggagg
301ccatgcttta tgcaattgac cagattaaca aggaccctga tctcctttcc aacatcactc
361tgggtgtccg catcctcgac acgtgctcta gggacaccta tgctttggag cagtctctaa
421cattcgtgca ggcattaata gagaaagatg cttcggatgt gaagtgtgct aatggagatc
481cacccatttt caccaagccc gacaagattt ctggcgtcat aggtgctgca gcaagctccg
541tgtccatcat ggttgctaac attttaagac tttttaagat acctcaaatc agctatgcat
601ccacagcccc agagctaagt gataacacca ggtatgactt tttctctcga gtggttccgc
661ctgactccta ccaagcccaa gccatggtgg acatcgtgac agcactggga tggaattatg
721tttcgacact ggcttctgag gggaactatg gtgagagcgg tgtggaggcc ttcacccaga
781tctcgaggga gattggtggt gtttgcattg ctcagtcaca gaaaatccca cgtgaaccaa
841gacctggaga atttgaaaaa attatcaaac gcctgctaga aacacctaat gctcgagcag
901tgattatgtt tgccaatgag gatgacatca ggaggatatt ggaagcagca aaaaaactaa
961accaaagtgg gcattttctc tggattggct cagatagttg gggatccaaa atagcacctg
1021tctatcagca agaggagatt gcagaagggg ctgtgacaat tttgcccaaa cgagcatcaa
1081ttgatggatt tgatcgatac tttagaagcc gaactcttgc caataatcga agaaatgtgt
1141ggtttgcaga attctgggag gagaattttg gctgcaagtt aggatcacat gggaaaagga
1201acagtcatat aaagaaatgc acagggctgg agcgaattgc tcgggattca tcttatgaac
1261aggaaggaaa ggtccaattt gtaattgatg ctgtatattc catggcttac gccctgcaca
1321atatgcacaa agatctctgc cctggataca ttggcctttg tccacgaatg agtaccattg
1381atgggaaaga gctacttggt tatattcggg ctgtaaattt taatggcagt gctggcactc
1441ctgtcacttt taatgaaaac ggagatgctc ctggacgtta tgatatcttc cagtatcaaa
1501taaccaacaa aagcacagag tacaaagtca tcggccactg gaccaatcag cttcatctaa
1561aagtggaaga catgcagtgg gctcatagag aacatactca cccggcgtct gtctgcagcc
1621tgccgtgtaa gccaggggag aggaagaaaa cggtgaaagg ggtcccttgc tgctggcact
1681gtgaacgctg tgaaggttac aactaccagg tggatgagct gtcctgtgaa ctttgccctc
1741tggatcagag acccaacatg aaccgcacag gctgccagct tatccccatc atcaaattgg
1801agtggcattc tccctgggct gtggtgcctg tgtttgttgc aatattggga atcatcgcca
1861ccacctttgt gatcgtgacc tttgtccgct ataatgacac acctatcgtg agggcttcag
1921gacgcgaact tagttacgtg ctcctaacgg ggatttttct ctgttattca atcacgtttt
1981taatgattgc agcaccagat acaatcatat gctccttccg acgggtcttc ctaggacttg
2041gcatgtgttt cagctatgca gcccttctga ccaaaacaaa ccgtatccac cgaatatttg
2101agcaggggaa gaaatctgtc acagcgccca agttcattag tccagcatct cagctggtga
2161tcaccttcag cctcatctcc gtccagctcc ttggagtgtt tgtctggttt gttgtggatc
2221ccccccacat catcattgac tatggagagc agcggacact agatccagag aaggccaggg
2281gagtgctcaa gtgtgacatt tctgatctct cactcatttg ttcacttgga tacagtatcc
2341TCTTGATGGT CACTTGTACT GTTTATGCCA TTAAAACGAG AGGTGTCCCA GAGACTTTCA
2401ATGAAGCCAA ACCTATTGGA TTTACCATGT ATACCACCTG CATCATTTGG TTAGCTTTCA
2461TCCCCATCTT TTTTGGTACA GCCCAGTCAG CAGAAAAGAT GTACATCCAG ACAACAACAC
2521TTACTGTCTC CATGAGTTTA AGTGCTTCAG TATTTCTGGG CATGCTCTAT ATGCCCAAGG
2581TTTATATTAT AATTTTTCAT CCAGAACAGA ATGTTCAAAA ACGCAAGAGG AGCTTCAAGG
2641CTGTGGTGAC AGCTGCCACC ATGCAAAGCA AACTGATCCA AAAAGGAAAT GACAGACCAA
2701ATGGCGAGGT GAAAAGTGAA CTCTGTGAGA GTCTTGAAAC CAACACTTCC TCTACCAAGA
2761CAACATATAT CAGTTACAGC AATCATTCAA TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG
2821ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2881GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT
2941AGTATTTATG GTTTTCTTGA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat
3001ttgtgaaaga tt

Download FASTA (ORF) (Full)