Construct: ORF TRCN0000477340

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009340.1_s317c1
Derived from:
ccsbBroadEn_00343
DNA Barcode:
GACGGATTCTGTAATGGCCCATTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
COL10A1 (1300)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477340
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1300 COL10A1 collagen type X alpha 1 chain NM_000493.4 100% 100%
2 human 1300 COL10A1 collagen type X alpha 1 chain XM_006715333.3 100% 100%
3 human 1300 COL10A1 collagen type X alpha 1 chain XM_011535432.3 100% 100%
4 human 1300 COL10A1 collagen type X alpha 1 chain XM_011535433.3 100% 100%
5 human 1300 COL10A1 collagen type X alpha 1 chain XM_017010248.1 100% 100%
6 mouse 12813 Col10a1 collagen, type X, alpha 1 NM_009925.4 85.7% 87.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2109
ORF length:
2040
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gctgccacaa ataccctttt tgctgctagt atccttgaac ttggttcatg
121gagtgtttta cgctgaacga taccaaatgc ccacaggcat aaaaggccca ctacccaaca
181ccaagacaca gttcttcatt ccctacacca taaagagtaa aggtatagca gtaagaggag
241agcaaggtac tcctggtcca ccaggccctg ctggacctcg agggcaccca ggtccttctg
301gaccaccagg aaaaccaggc tacggaagtc ctggactcca aggagagcca gggttgccag
361gaccaccggg accatcagct gtagggaaac caggtgtgcc aggactccca ggaaaaccag
421gagagagagg accatatgga ccaaaaggag atgttggacc agctggccta ccaggacccc
481ggggcccacc aggaccacct ggaatccctg gaccggctgg aatttctgtg ccaggaaaac
541ctggacaaca gggacccaca ggagccccag gacccagggg ctttcctgga gaaaagggtg
601caccaggagt ccctggtatg aatggacaga aaggggaaat gggatatggt gctcctggtc
661gtccaggtga gaggggtctt ccaggccctc agggtcccac aggaccatct ggccctcctg
721gagtgggaaa aagaggtgaa aatggggttc caggacagcc aggcatcaaa ggtgatagag
781gttttccggg agaaatggga ccaattggcc caccaggtcc ccaaggccct cctggggaac
841gagggccaga aggcattgga aagccaggag ctgctggagc cccaggccag ccagggattc
901caggaacaaa aggtctccct ggggctccag gaatagctgg gcccccaggg cctcctggct
961ttgggaaacc aggcttgcca ggcctgaagg gagaaagagg acctgctggc cttcctgggg
1021gtccaggtgc caaaggggaa caagggccag caggtcttcc tgggaagcca ggtctgactg
1081gaccccctgg gaatatggga ccccaaggac caaaaggcat cccgggtagc catggtctcc
1141caggccctaa aggtgagaca gggccagctg ggcctgcagg ataccctggg gctaagggtg
1201aaaggggttc ccctgggtca gatggaaaac cagggtaccc aggaaaacca ggtctcgatg
1261gtcctaaggg taacccaggg ttaccaggtc caaaaggtga tcctggagtt ggaggacctc
1321ctggtctccc aggccctgtg ggcccagcag gagcaaaggg aatgcccgga cacaatggag
1381aggctggccc aagaggtgcc cctggaatac caggtactag aggccctatt gggccaccag
1441gcattccagg attccctggg tctaaagggg atccaggaag tcccggtcct cctggcccag
1501ctggcatagc aactaagggc ctcaatggac ccaccgggcc accagggcct ccaggtccaa
1561gaggccactc tggagagcct ggtcttccag ggccccctgg gcctccaggc ccaccaggtc
1621aagcagtcat gcctgagggt tttataaagg caggccaaag gccCAGTCTT TCTGGGACCC
1681CTCTTGTTAG TGCCAACCAG GGGGTAACAG GAATGCCTGT GTCTGCTTTT ACTGTTATTC
1741TCTCCAAAGC TTACCCAGCA ATAGGAACTC CCATACCATT TGATAAAATT TTGTATAACA
1801GGCAACAGCA TTATGACCCA AGGACTGGAA TCTTTACTTG TCAGATACCA GGAATATACT
1861ATTTTTCATA CCACGTGCAT GTGAAAGGGA CTCATGTTTG GGTAGGCCTG TATAAGAATG
1921GCACCCCTGT AATGTACACC TATGATGAAT ACACCAAAGG CTACCTGGAT CAGGCTTCAG
1981GGAGTGCCAT CATCGATCTC ACAGAAAATG ACCAGGTGTG GCTCCAGCTT CCCAATGCCG
2041AGTCAAATGG CCTATACTCC TCTGAGTATG TCCACTCCTC TTTCTCAGGA TTCCTAGTGG
2101CTCCAATGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
2161CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
2221TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGACGGA TTCTGTAATG GCCCATTTAC
2281GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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