Transcript: Human XM_006715333.3

PREDICTED: Homo sapiens collagen type X alpha 1 chain (COL10A1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
COL10A1 (1300)
Length:
4022
CDS:
816..2858

Additional Resources:

NCBI RefSeq record:
XM_006715333.3
NBCI Gene record:
COL10A1 (1300)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006715333.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000082798 CCTCACTTATTAAAGCACAAA pLKO.1 3836 3UTR 100% 4.950 6.930 N COL10A1 n/a
2 TRCN0000082802 CCGAGTCAAATGGCCTATACT pLKO.1 2785 CDS 100% 5.625 4.500 N COL10A1 n/a
3 TRCN0000415520 CATCCAGGAGGTATCATATAA pLKO_005 3290 3UTR 100% 15.000 10.500 N COL10A1 n/a
4 TRCN0000082799 CCCTACACCATAAAGAGTAAA pLKO.1 948 CDS 100% 13.200 9.240 N COL10A1 n/a
5 TRCN0000417638 GATTTGAGAAACTCGGCATTT pLKO_005 3218 3UTR 100% 10.800 7.560 N COL10A1 n/a
6 TRCN0000082800 CCTGTAATGTACACCTATGAT pLKO.1 2673 CDS 100% 5.625 3.938 N COL10A1 n/a
7 TRCN0000082801 GCATCAAAGGTGATAGAGGTT pLKO.1 1510 CDS 100% 2.640 1.848 N COL10A1 n/a
8 TRCN0000430740 GATACCAGGAATATACTATTT pLKO_005 2591 CDS 100% 13.200 7.920 N COL10A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006715333.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00343 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00343 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000477340 GACGGATTCTGTAATGGCCCATTT pLX_317 22.1% 100% 100% V5 n/a
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