Construct: ORF TRCN0000477341
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000925.1_s317c1
- Derived from:
- ccsbBroadEn_14308
- DNA Barcode:
- GCCTGCTTTTAAACAATCATGTAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ATP13A4 (84239)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477341
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84239 | ATP13A4 | ATPase 13A4 | XM_017007319.1 | 90.8% | 43.2% | 1202_1203insA;1208_1209insG;2520_2772delinsG |
| 2 | human | 84239 | ATP13A4 | ATPase 13A4 | XM_011513232.2 | 88.4% | 42% | 1202_1203insA;1208_1209insG;2520_2847delinsG |
| 3 | human | 84239 | ATP13A4 | ATPase 13A4 | XR_001740325.1 | 76.7% | (many diffs) | |
| 4 | human | 84239 | ATP13A4 | ATPase 13A4 | NM_032279.4 | 70.1% | 33.4% | 1202_1203insA;1208_1209insG;2520_3588delinsG |
| 5 | human | 84239 | ATP13A4 | ATPase 13A4 | XR_001740324.2 | 63% | (many diffs) | |
| 6 | human | 84239 | ATP13A4 | ATPase 13A4 | XM_017007318.1 | 61% | 24.3% | (many diffs) |
| 7 | human | 84239 | ATP13A4 | ATPase 13A4 | XR_924191.3 | 58.3% | (many diffs) | |
| 8 | human | 84239 | ATP13A4 | ATPase 13A4 | XR_002959602.1 | 49.4% | (many diffs) | |
| 9 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | NM_001164613.1 | 81.5% | 39.7% | (many diffs) |
| 10 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XM_017316956.1 | 81.5% | 39.7% | (many diffs) |
| 11 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XM_006522054.3 | 77.2% | 37.3% | (many diffs) |
| 12 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XM_006522053.3 | 75.9% | 36.6% | (many diffs) |
| 13 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XM_006522051.3 | 64.5% | 31.1% | (many diffs) |
| 14 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XM_017316955.1 | 63.8% | 30.8% | (many diffs) |
| 15 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | NM_001164612.1 | 60.7% | 29.3% | (many diffs) |
| 16 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | NM_172613.4 | 59.2% | 29.9% | (many diffs) |
| 17 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XM_006522048.3 | 57.7% | 26.1% | (many diffs) |
| 18 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XR_384556.3 | 50.8% | (many diffs) | |
| 19 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XR_001781812.1 | 42.1% | (many diffs) | |
| 20 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XR_384557.3 | 41.2% | (many diffs) | |
| 21 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XM_006522055.3 | 22.5% | 3.2% | (many diffs) |
| 22 | mouse | 224079 | Atp13a4 | ATPase type 13A4 | XM_006522056.3 | 20.3% | 9.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1287
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggacacttt gagaagggcc agcacgctct gctcaatgaa ggagaagaga 121 atgagatgga gatatttggc tatcggactc aaggctgccg gaaaagtctc tgccttgccg 181 gatccatctt ctcatttgga atcctcccct tggtgtttta ctggagacca gcatggcacg 241 tatgggcaca ttgtgtccca tgttccttgc aagaagcaga cactgtgttg ctgaggacaa 301 cggatgaatt ccaaatttac tcttggaaaa aggtaatatg gatctacctg tcagcattaa 361 acagcgcatt tggtctcact cctgaccacc ctctcatgac agatgaggag tatatcataa 421 atagagccat aagaaagcca gacctaaagg tgagatgcat caaagtgcag aaaataagat 481 atgtttggaa ctacttagaa ggacagttcc agaaaattgg ttctttggaa gactggctta 541 gttctgccaa gatacatcaa aaatttggat caggcttgac aagagaagaa caggagatta 601 ggaggttaat atgtgggcct aatactatcg atgttgaagt tacaccaatt tggaaactgc 661 tcatcaagga ggttctaaat ccattttata tatttcaact cttcagtgtc tgtttgtggt 721 ttagtgaaga ctataaggaa tatgcttttg ccatcataat catgtccata atttccatat 781 ctttgacagt atatgatctc agagagcaat ctgtaaaact ccaccatctc gtcgagtcac 841 ataatagcat tacggtctct gtatgtggga gaaaagctgg agttcaagag ctggaatcac 901 gcgtcctggt gcctggagat ttattaattt tgacagggaa caaagtgcta atgccatgtg 961 atgccgttct gattgaaggc agctgtgtgg tggatgaagg catgctgaca ggagaaagta 1021 ttccagtcac caaaactccg ttacccaaga tggatagctc tgtgccctgg aaaacacaga 1081 gtgaagcgga ttacaagcgg catgtcctct tctgtggaac agaggttatc caggccaagg 1141 cagcttgctc tgggaccgtg agagccgtgg tactgcagac tggattcaac actgcaaagg 1201 gagaccttgt gagatccatt ctctacccta agccagtgaa ttttcagttg tacagggatg 1261 ccatcaggtt acctcctggt gccttgtagg aacagccacc attgggatga tctatactct 1321 gtgtgtctat gtgcttagtg gggaacctcc agaggaggtg gtgaggaaag cccttgacgt 1381 catcacaatt gcggttcctc cggctctacc tgctgctctg accacaggca ttatctatgc 1441 ccagaggagg ctgaagaaga gaggcatctt ctgcattagc ccccagagga tcaacgtatg 1501 tggacagtta aaccttgtct gctttgacaa gacaggcacc ttaacaaggg acggcttgga 1561 cctctgggga gtcgtgtcct gtgataggaa tggctttcag gaagttcaca gctttgcctc 1621 aggccaggct ttgccatggg gcccactgtg tgcagcgatg gccagctgcc actctctgat 1681 ccttcttgat gggaccatcc agggagaccc tctggacctc aaaatgtttg aagccaccac 1741 ctgggaaatg gctttttctg gggacgattt ccacatcaag ggagtgccgg cacatgccat 1801 ggtagttaag ccctgcagaa cagccagcca ggtcccagtg gaaggaattg caatcctgca 1861 tcagttccca ttctcatcgg cactgcaaag aatgacagtc attgtccaag agatgggagg 1921 tgaccgactg gcattcatga aaggtgcacc agagagggtg gccagctttt gccaacctga 1981 gacagtaccc actagttttg ttagcgaact tcagatttac acgacacagg gcttccgagt 2041 catagcactg gcctacaaga agctggaaaa tgaccatcac gctactacct tgacgaggga 2101 gacggtagaa tcagacctga tatttctggg gctgctgatc ttggagaatc gattgaagga 2161 agagacaaaa cctgtcttgg aagagctcat ctcagcccgg ataaggactg taatgatcac 2221 aggtgacaat cttcagactg caataacagt ggccagaaAA TCTGGAATGG TTTCTGAAAG 2281 CCAGAAAGTC ATTCTCATTG AGGCAAATGA AACCACCGGG TCCTCATCAG CATCTATATC 2341 TTGGACGTTA GTAGAAGAGA AGAAACACAT TATGTATGGG AATCAGGACA ATTACATTAA 2401 CATCAGGGAT GAAGTCTCTG ATAAAGGCAG AGAAGGAAGT TACCATTTTG CCCTAACTGG 2461 AAAATCCTTT CATGTTATAA GTCAACATTT CAGCAGCCTA CTGCCAAAGA TATTGATCAA 2521 TGGGACCATC TTTGCAAGAA TGTCTCCTGG GCAGAAGTCC AGTCTGGTGG AAGAATTTCA 2581 GAAACTGGAG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA 2641 CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC 2701 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCCT GCTTTTAAAC AATCATGTAG 2761 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt