Construct: ORF TRCN0000477346
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017036.1_s317c1
- Derived from:
- ccsbBroadEn_14321
- DNA Barcode:
- CTCAAGGAAGTCTACGTTTCCCAG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC49A4 (84925)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477346
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84925 | SLC49A4 | solute carrier family 49 me... | NM_032839.3 | 99.9% | 99.1% | 1422delG |
| 2 | human | 84925 | SLC49A4 | solute carrier family 49 me... | XR_001740333.2 | 14.2% | 1_135del;1557delG;1570_10079del | |
| 3 | mouse | 224132 | Slc49a4 | solute carrier family 49 me... | NM_153550.4 | 87.6% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1500
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctctcgctgg agcagcgaag aggagaggca gccgctgctg gggcccgggc 121 tcgggcctgg gctgggggcc tcctggagaa gccgggaggc ggcggcggcg gcgctgcccg 181 cggcggtccc gggtcccggg cgggtatacg ggcgccgctg gctggtgctg ctgctcttct 241 cgctgctggc gttcgttcag ggcctggtct ggaacacctg gggtcccatc cagaactcgg 301 cgcgccaggc ctacggcttc tccagctggg acatcgcgct gctcgtgctg tgggggccca 361 tcggcttcct gccctgcttc gcgttcatgt ggctcctgga caagagaggt ctccggataa 421 ctgtgctcct gacatccttc cttatggttt tgggaactgg tctaagatgc atacctatat 481 cagacttaat ccttaaaaga agattaattc atggaggaca gatgttaaat ggattggcag 541 gtccaactgt aatgaatgca gcaccatttc tctctacgac gtggttttct gcagatgaaa 601 gggccacagc cacagctatt gcatcaatgc tcagttatct tgggggagca tgtgcatttt 661 tagttggacc acttgttgtt ccagctccca atgggacatc acctcttctt gctgcagaga 721 gcagcagggc gcatattaaa gatcgcatag aggctgtgtt atatgcagaa tttggagttg 781 tctgcttaat attttctgca acactagctt atttcccacc ccgacctcct cttcctccca 841 gtgttgctgc agctagccag cggctgagtt atcggagaag cgtttgtaga ttattaagca 901 attttcgatt tttgatgatt gctttagcat atgccatacc acttggtgta tttgctggct 961 ggtctggagt tctggactta attttaacac cagcgcatgt cagccaagta gatgctggct 1021 ggattggatt ttggtccata gttggaggct gtgttgttGG AATAGCTATG GCAAGGTTTG 1081 CAGATTTTAT CAGGGGTATG CTGAAACTAA TTCTTCTCCT CCTGTTTTCG GGAGCTACAC 1141 TGTCATCCAC GTGGTTCACC CTGACCTGTT TGAACAGCAT CACACACCTA CCTTTAACCA 1201 CAGTGACATT GTATGCCTCC TGTATTCTCC TGGGAGTGTT CTTGAATAGC AGCGTGCCTA 1261 TATTTTTTGA GCTTTTTGTG GAAACTGTCT ACCCAGTTCC AGAAGGAATT ACTTGTGGAG 1321 TTGTCACTTT TTTAAGTAAT ATGTTTATGG GAGTACTTTT ATTTTTTCTC ACATTTTATC 1381 ATACAGAGTT GTCTTGGTTC AACTGGTGCC TTCCCGGGTC GTGTTTGCTC AGTCTCCTCC 1441 TCATTCTGTG CTTCAGGGAA TCCTATGACA GACTCTATCT TGATGTGTTG TCTCCGTTTA 1501 CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 1561 TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 1621 TTATATATCT TGTGGAAAGG ACGACTCAAG GAAGTCTACG TTTCCCAGAC GCGTTAAGTC 1681 gacaatcaac ctctggatta caaaatttgt gaaagatt