Construct: ORF TRCN0000477372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006910.1_s317c1
- Derived from:
- BRDN0000392935
- DNA Barcode:
- AACGCCATTCTTCTCATAGTACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEFSEC (60678)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477372
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60678 | EEFSEC | eukaryotic elongation facto... | NM_021937.5 | 94.6% | 94.1% | (many diffs) |
| 2 | human | 60678 | EEFSEC | eukaryotic elongation facto... | XM_024453693.1 | 87.3% | 86.8% | (many diffs) |
| 3 | human | 60678 | EEFSEC | eukaryotic elongation facto... | XM_024453692.1 | 87.2% | 86.7% | (many diffs) |
| 4 | human | 60678 | EEFSEC | eukaryotic elongation facto... | XM_005247696.3 | 85.5% | 84.8% | (many diffs) |
| 5 | human | 60678 | EEFSEC | eukaryotic elongation facto... | XM_024453694.1 | 79.8% | 74.7% | (many diffs) |
| 6 | human | 60678 | EEFSEC | eukaryotic elongation facto... | XM_024453695.1 | 79.5% | 77.1% | (many diffs) |
| 7 | human | 60678 | EEFSEC | eukaryotic elongation facto... | XM_024453696.1 | 60.3% | 58.5% | (many diffs) |
| 8 | human | 60678 | EEFSEC | eukaryotic elongation facto... | XM_011513066.2 | 57.2% | 57.1% | 0_1ins726;530_531delTCinsGG;970G>A |
| 9 | mouse | 65967 | Eefsec | eukaryotic elongation facto... | NM_023060.3 | 79.1% | 82% | (many diffs) |
| 10 | mouse | 65967 | Eefsec | eukaryotic elongation facto... | XM_006506464.1 | 55.1% | 56.4% | (many diffs) |
| 11 | mouse | 65967 | Eefsec | eukaryotic elongation facto... | XM_006506465.3 | 48.8% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1773
- ORF length:
- 1707
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atcctgctcc acgggctact ttgacaagca gccgcagagc cgcgagcgcg 121 gcatcacgct cgatctgggc ttctcgtgct tctcggtgcc gctgcccgcg cgcctgcggt 181 cgtctttgcc cgagttccag gcagcgcccg aggccgagcc cgagcccggc gagccactgc 241 ttcaggtcac gctggtcgac tgccccgggc acgcctccct catccggacc atcatcggcg 301 gggcccagat cattgatctg atgatgctgg tcatcgatgt gaccaagggg atgcagaccc 361 agtcagcgga atgccttgtg atcggccaga ttgcctgcca gaagctggtc gtggtgctga 421 acaaaataga cctcttacct gaaggaaaga gacaggcagc aattgataaa atgaccaaga 481 aaatgcagaa gaccctagag aacaccaagt tccgaggtgc accgattata cccgtggcgg 541 ccaagccggg gggaccagag gcccccgaaa ctgaagctcc acagggcatt ccagagctca 601 ttgagctcct gacgtcccag atttccatcc caacgagaga cccctcggga ccgttcctca 661 tgtctgtgga ccactgtttc tccatcaaag gccaaggcac tgtgatgaca gggaccatcc 721 tttcaggctc catcagcctc ggtgacagtg tggagatccc tgccctcaag gtggtgaaga 781 aggtgaagtc catgcagatg ttccacatgc ccatcacttc agccatgcaa ggagaccggc 841 tgggcatctg cgtcacccag tttgacccta agctgctgga gcgcgggttg gtgtgtgccc 901 ccgagtccct gcacactgtc catgcggccc tcatctctgt ggaaaagata ccgtatttcc 961 gggggcccct gcaaaccaag gccaagttcc acattacagt gggccatgaa acagtcatgg 1021 gccggttgat gttcttcagt cctgctccag ataactttga ccaggagcct atactggact 1081 ctttcaactt ctctcaagaa taccttttcc aggagcagta cctgtccaag gatttgacac 1141 cagcagtgac agacaatgat gaggccgaca agaaggccgg ccaggccaca gagggccatt 1201 gtcctcggca gcagtgggcc ctggtggagt ttgagaagcc cgtcacctgc cctcggctgt 1261 gcctggtgat tggctccagg ctagatgcgg acattcacac caacacgtgc cggctagcct 1321 ggcatggcat cctgctccac gggctagagg acaggaacTA CGCCGACAGC TTCCTGCCCA 1381 GGCTGAAGGT GTACAAGCTG AAGCACAAGC ATGGCCTTGT GGAGCGGGCG ATGGATGACT 1441 ACAGTGTGAT CGGCCGCTCC CTGTTCAAAA AGGAAACCAA CATCCAGCTC TTCGTGGGGC 1501 TCAAGGTGCA CTTGTCCACT GGGGAACTGG GCATCATCGA CAGTGCCTTC GGCCAGAGCG 1561 GCAAGTTCAA GATCCACATC CCAGGTGGCC TCAGCCCCGA GTCCAAGAAG ATCCTGACAC 1621 CCGCCCTCAA GAAGCGGGCC CGGGCTGGCC GTGGGGAGGC CACCAGGCAG GAGGAGAGCG 1681 CCGAGCGGAG CGAGCCCTCA CAGCATGTGG TGCTCAGCCT GACTTTCAAG CGTTATGTCT 1741 TCGACACCCA CAAGCGCATG ATTCAGTCTC CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1801 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1861 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 1921 CGCCATTCTT CTCATAGTAC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1981 ttgtgaaaga tt