Construct: ORF TRCN0000477382
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005039.1_s317c1
- Derived from:
- ccsbBroadEn_04927
- DNA Barcode:
- TCCCTCGAGGGGGTGTACCTGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HDX (139324)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477382
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139324 | HDX | highly divergent homeobox | NM_001177479.2 | 100% | 100% | |
| 2 | human | 139324 | HDX | highly divergent homeobox | NM_144657.5 | 100% | 100% | |
| 3 | human | 139324 | HDX | highly divergent homeobox | XM_011530863.2 | 100% | 100% | |
| 4 | human | 139324 | HDX | highly divergent homeobox | XM_011530864.1 | 100% | 100% | |
| 5 | human | 139324 | HDX | highly divergent homeobox | XM_017029274.1 | 100% | 100% | |
| 6 | human | 139324 | HDX | highly divergent homeobox | XM_017029275.1 | 97.3% | 97.3% | 1251_1252ins54 |
| 7 | human | 139324 | HDX | highly divergent homeobox | NM_001177478.2 | 91.5% | 91.5% | 0_1ins174 |
| 8 | human | 139324 | HDX | highly divergent homeobox | XM_006724619.1 | 91.5% | 91.5% | 0_1ins174 |
| 9 | human | 139324 | HDX | highly divergent homeobox | XM_024452336.1 | 65.9% | 62.2% | (many diffs) |
| 10 | human | 139324 | HDX | highly divergent homeobox | XR_001755652.1 | 57% | 1_118del;2189_3626del | |
| 11 | human | 139324 | HDX | highly divergent homeobox | XR_001755654.1 | 56.2% | (many diffs) | |
| 12 | human | 139324 | HDX | highly divergent homeobox | XR_001755653.1 | 55.5% | 1_118del;1369_1370ins54;2135_3572del | |
| 13 | human | 139324 | HDX | highly divergent homeobox | XR_938392.1 | 55.4% | 1_227del;2298_3735del | |
| 14 | human | 139324 | HDX | highly divergent homeobox | XR_001755651.1 | 46.7% | 1_118del;2189_4426del | |
| 15 | mouse | 245596 | Hdx | highly divergent homeobox | NM_001080549.2 | 90.7% | 90.6% | (many diffs) |
| 16 | mouse | 245596 | Hdx | highly divergent homeobox | NM_001290459.1 | 82.5% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2139
- ORF length:
- 2070
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatctacgt tctgtattta ctgtagaaca acaaaggatt ttacagcgtt 121 attatgaaaa tggaatgaca aatcaaagta aaaattgctt tcagctcata ttacagtgtg 181 cacaggagac taagctggac ttcagtgtag tcaggacgtg ggttggcaat aagagaagaa 241 agatgagtag taagaactct gaatctggaa cagcaacaac aggaacctct ttgtcagctc 301 cagacatcac agtcagaaat gtggttaata ttgctcgacc ctcaagccag cagtcttctt 361 ggacatctgc caataatgat gtcattgtaa ctggtatata cagtccagcc agttcatcaa 421 gtaggcaagg aacaaacaaa catacagaca cacaaattac agaagcacat aaaatcccta 481 ttcagaaaac agccactaaa aatgatactg agtttcagtt acacattcct gtccaaagac 541 aagtagcaca ctgtaaaaat gcttccctac tcctaggtga aaaaacaatt attttgtcaa 601 gacagacaag tgtgctaaat gctggaaact cagtattcaa tcacgcaaag aaaaactatg 661 gaaactcttc agtacaagct tctgaaatga cagtacctca aaagccttct gtgtgccacc 721 gaccttgtaa aattgaacca gttgggattc aaaggtcata taagcctgaa cacacaggcc 781 cagcattaca taacttatgt gggcaaaagc caactattag agacccttac tgtagaacac 841 aaaacttgga aatccgtgaa gtgttttcat tggcagttag cgattacccc cagagaattc 901 tgggaggaaa tgccccacag aagcctagct cagcagaagg aaattgtttg tccattgcaa 961 tggagactgg agatgctgag gatgaatatg ccagagagga agagctggca tcgatgagag 1021 cacagatacc aagctattcg agattttatg aaagtggcag ttcccttcga gctgagaacc 1081 aaagtacaac cttgcccgga ccaggaagaa atatgccaaa ttcacaaatg gtgaatatta 1141 gagatatgtc agacaatgta ctgtatcaaa acagaaacta ccatttgaca ccacggacct 1201 cattacatac agcatctagt acaatgtaca gtaataccaa tccattacgg agtaattttt 1261 ctcctcattt tgcatcatca aaccaattga gattatcaca aaaccaaaac aattaccaga 1321 tttcaggaaa ccttactgtg ccttggatta cagggtgttc tagaaaaaga gcactacagg 1381 accgcactca gttcagtgac cgagacttag ccacccttaa gaagtattgg gacaatggca 1441 tgaccagcct gggctctgtt tgtagagaga aaattgaagc tgtggcaact gaattaaatg 1501 ttgactgtga aatagttcgg acttggattg ggaatcgaag aaggaaatat cgtttaatgg 1561 ggattgaagt tccacctcca agaggaggcc ctgctgattt ctctgagcag cctgagtctg 1621 gttctttatc tgcactcaca ccaggagagg aagctgggcc tgaagtagga gaggataatg 1681 acagaaatga tgaagtatcc atctgtttgt ctgaaggaag ctcTCAAGAA GAGCCCAATG 1741 AAGTTGTTCC GAATGATGCA AGGGCTCATA AGGAAGAGGA CCACCATGCA GTAACCACAG 1801 ATAATGTGAA AATAGAAATT ATTGATGATG AAGAAAGTGA CATGATAAGT AATTCTGAAG 1861 TAGAACAAGT AAACTCTTTC TTGGATTATA AGAATGAAGA AGTCAAATTC ATTGAAAATG 1921 AGCTCGAGAT TCAAAAGCAA AAATACTTTA AACTTCAGAC TTTTGTTAGA AGCTTGATAT 1981 TAGCAATGAA AGCTGATGAT AAGGAACAAC AGCAGGCACT GCTGTCAGAT TTACCTCCTG 2041 AATTAGAGGA AATGGATTTC AATCATGCCT CACTGGAGCC TGATGATACC TCATTCAGTG 2101 TATCTTCTTT GTCAGAGAAA AATGTCTCAG AAAGTTTGTT GCCAACTTTC TTGTACAAAG 2161 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2221 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2281 ACGATCCCTC GAGGGGGTGT ACCTGGAAAC GCGTTAAGTC gacaatcaac ctctggatta 2341 caaaatttgt gaaagatt