Construct: ORF TRCN0000477392
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007776.1_s317c1
- Derived from:
- ccsbBroadEn_13279
- DNA Barcode:
- ACAGGCCTATCCCGGCGGGGTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPM1L (151742)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477392
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151742 | PPM1L | protein phosphatase, Mg2+/M... | NM_001317911.2 | 100% | 100% | |
| 2 | human | 151742 | PPM1L | protein phosphatase, Mg2+/M... | NM_001317912.2 | 77.6% | 77.6% | 0_1ins156 |
| 3 | human | 151742 | PPM1L | protein phosphatase, Mg2+/M... | NM_139245.4 | 63.9% | 63.3% | (many diffs) |
| 4 | human | 151742 | PPM1L | protein phosphatase, Mg2+/M... | XM_011512440.3 | 17.3% | 16.6% | (many diffs) |
| 5 | human | 151742 | PPM1L | protein phosphatase, Mg2+/M... | NR_134243.1 | 6.3% | (many diffs) | |
| 6 | mouse | 242083 | Ppm1l | protein phosphatase 1 (form... | XM_006501470.3 | 81.6% | 88.6% | (many diffs) |
| 7 | mouse | 242083 | Ppm1l | protein phosphatase 1 (form... | XM_011240119.2 | 74.8% | 69.2% | (many diffs) |
| 8 | mouse | 242083 | Ppm1l | protein phosphatase 1 (form... | NM_178726.3 | 57.6% | 62.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 768
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctgctttt tcaacaactg cagctgaata tgtaaaatct cgactcccag 121 aggcccttaa acagcatctt caggactacg agaaagacaa agaaaatagt gtattatctt 181 accagaccat ccttgaacag cagattttgt caattgaccg agaaatgcta gaaaaattga 241 ctgtatccta tgatgaagca ggcacaacgt gtttgattgc tctgctatca gataaagacc 301 tcactgtggc caacgtgggt gactcgcgcg gggTCCTGTG TGACAAAGAT GGGAACGCTA 361 TTCCTTTGTC TCATGATCAC AAGCCTTACC AGTTGAAGGA AAGAAAGAGG ATAAAGAGAG 421 CAGGTGGTTT CATCAGTTTC AATGGCTCCT GGAGGGTCCA GGGAATCCTG GCCATGTCTC 481 GGTCCCTGGG GGATTATCCG CTGAAAAATC TCAACGTGGT CATCCCAGAC CCAGACATCC 541 TGACCTTTGA CCTGGACAAG CTTCAGCCTG AGTTCATGAT CTTGGCATCA GATGGTCTCT 601 GGGATGCTTT CAGCAATGAA GAAGCAGTTC GATTCATCAA GGAGCGCTTG GATGAACCTC 661 ACTTTGGGGC CAAGAGCATA GTTTTACAGT CATTTTACAG AGGCTGCCCT GACAATATAA 721 CAGTCATGGT GGTGAAGTTC AGAAATAGCA GCAAAACAGA AGAGCAGTTG CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGAACAGGCC TATCCCGGCG GGGTGTAACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt