Transcript: Human NM_139245.4

Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1L (PPM1L), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
PPM1L (151742)
Length:
10906
CDS:
79..1161

Additional Resources:

NCBI RefSeq record:
NM_139245.4
NBCI Gene record:
PPM1L (151742)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_139245.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000355917 GATTGCTCTGCTATCAGATAA pLKO_005 666 CDS 100% 13.200 18.480 N PPM1L n/a
2 TRCN0000002699 ACGTGTTTGATTGCTCTGCTA pLKO.1 658 CDS 100% 2.640 3.696 N PPM1L n/a
3 TRCN0000355916 CCTGGTGGTCTTAGGTCTATA pLKO_005 1285 3UTR 100% 13.200 9.240 N PPM1L n/a
4 TRCN0000355852 TCTCAGCTGCCTTAGACTAAA pLKO_005 1174 3UTR 100% 13.200 9.240 N PPM1L n/a
5 TRCN0000355853 ATCTTCAGGACTACGAGAAAG pLKO_005 527 CDS 100% 10.800 7.560 N PPM1L n/a
6 TRCN0000002698 AGGTGGTTTCATCAGTTTCAA pLKO.1 813 CDS 100% 5.625 3.938 N PPM1L n/a
7 TRCN0000002696 CCCATGAGATTTGCTTACTTT pLKO.1 2929 3UTR 100% 5.625 3.938 N PPM1L n/a
8 TRCN0000002700 CGTGTTTGATTGCTCTGCTAT pLKO.1 659 CDS 100% 4.950 3.465 N PPM1L n/a
9 TRCN0000080877 CCTATGATGAAGCAGGCACAA pLKO.1 638 CDS 100% 4.050 2.835 N Ppm1l n/a
10 TRCN0000002697 GTGACAAAGATGGGAACGCTA pLKO.1 731 CDS 100% 2.640 1.848 N PPM1L n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 5822 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 5822 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_139245.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13279 pDONR223 100% 63.9% 63.3% None (many diffs) n/a
2 ccsbBroad304_13279 pLX_304 0% 63.9% 63.3% V5 (many diffs) n/a
3 TRCN0000477392 ACAGGCCTATCCCGGCGGGGTGTA pLX_317 61.6% 63.9% 63.3% V5 (many diffs) n/a
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