Construct: ORF TRCN0000477419
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006237.1_s317c1
- Derived from:
- ccsbBroadEn_01816
- DNA Barcode:
- CGGGCTCGAAACTTATCCGTGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZKSCAN8 (7745)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477419
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7745 | ZKSCAN8 | zinc finger with KRAB and S... | NM_001278119.2 | 100% | 100% | |
| 2 | human | 7745 | ZKSCAN8 | zinc finger with KRAB and S... | NM_006298.4 | 100% | 100% | |
| 3 | human | 7745 | ZKSCAN8 | zinc finger with KRAB and S... | XM_011514870.2 | 100% | 100% | |
| 4 | human | 7745 | ZKSCAN8 | zinc finger with KRAB and S... | XM_017011266.2 | 100% | 100% | |
| 5 | human | 7745 | ZKSCAN8 | zinc finger with KRAB and S... | NM_001278121.1 | 67.6% | 67.6% | 0_1ins561 |
| 6 | human | 7745 | ZKSCAN8 | zinc finger with KRAB and S... | NM_001278122.2 | 67.6% | 67.6% | 0_1ins561 |
| 7 | human | 7745 | ZKSCAN8 | zinc finger with KRAB and S... | XM_011514873.2 | 67.6% | 67.6% | 0_1ins561 |
| 8 | human | 7745 | ZKSCAN8 | zinc finger with KRAB and S... | XM_011514874.2 | 67.6% | 67.6% | 0_1ins561 |
| 9 | mouse | 93681 | Zkscan8 | zinc finger with KRAB and S... | NM_139141.4 | 86.4% | 86.2% | (many diffs) |
| 10 | mouse | 93681 | Zkscan8 | zinc finger with KRAB and S... | XM_017315646.1 | 85% | 84.7% | (many diffs) |
| 11 | mouse | 93681 | Zkscan8 | zinc finger with KRAB and S... | NM_001251833.1 | 77.7% | 77.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1803
- ORF length:
- 1734
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgaagaa tcaagaaagc cttcagcccc atccccacca gaccagactc 121 ctgaagagga tcttgtaatc gtcaaggtag aggaggatca tggttgggac caggaatcta 181 gtctgcatga aagtaaccct cttggccaag aagtgttccg cctgcgcttc aggcagttac 241 gctaccagga gacactagga ccccgagaag ctctgatcca actacgggcc ctttgccatc 301 agtggctgag gccagatttg aacaccaagg aacagatcct ggagctgctg gtgctggagc 361 agttcttgac catcctacct gaggagctcc agacactggt taaggaacat cagctagaga 421 acggagagga ggtggtgacc ctattagagg atttggaaag gcagattgat atactaggac 481 gaccagtctc agctcgcgta catggacata gggtactctg ggaggaggta gtacattcag 541 catctgcacc agagcctcca aatactcagc tccaatctga ggcaacccaa cataaatctc 601 cagtgcccca agagtcacaa gagagagcca tgtctacttc ccagagtcct actcgttccc 661 agaaaggaag ttctggagac caggaaatga cagctacact tctcacagca gggttccaga 721 ctttggagaa gattgaagac atggctgtgt cccttattcg agaggagtgg cttcttgatc 781 catcacagaa ggatctgtgt agagataaca ggccagaaaa tttcagaaac atgttctccc 841 tgggtggtga gaccaggagt gagaacaggg aattagcttc aaaacaggta atatctactg 901 gaatccagcc acatggagag acagctgcca aatgcaacgg ggatgttatc aggggtcttg 961 agcatgaaga agcccgagac cttctgggca gattagagag gcagcgggga aatcccacac 1021 aagagagacg acataaatgt gatgaatgtg ggaaaagctt tgctcagagc tcaggccttg 1081 ttcgccactg gagaatccac actggggaga aaccctatca gtgtaatgtg tgtggtaaag 1141 ccttcagtta caggtcagcc cttctttcac atcaggatat ccacaacaaa gtaaaacgct 1201 atcactgtaa ggagtgtggc aaagccttca gtcagaacac aggcctgatt ctgcaccaga 1261 gaatccacac tggggagaag ccatatcagt gcaatcagtg tgggaaggct ttcagtcaga 1321 gtgcgggcct tattctgcac cagagaatcc acagtggaga gagaccctat gaatgtaatg 1381 agtgtgggaa agctttcagt catagctcac accTCATTGG ACATCAGAGA ATCCACACTG 1441 GGGAGAAGCC CTATGAGTGT GATGAGTGTG GGAAAACCTT CAGGCGGAGC TCACATCTTA 1501 TTGGTCATCA GAGGAGCCAC ACTGGGGAGA AACCCTACAA ATGCAATGAG TGTGGGAGGG 1561 CCTTCAGTCA GAAGTCAGGC CTTATTGAAC ATCAGAGAAT CCACACTGGA GAAAGACCCT 1621 ATAAATGTAA AGAATGTGGG AAAGCTTTCA ATGGGAACAC TGGTCTCATT CAACACCTGA 1681 GAATTCACAC AGGGGAGAAG CCCTACCAAT GTAATGAGTG TGGGAAAGCC TTTATTCAGA 1741 GGTCAAGTCT CATTCGACAT CAGAGAATCC ACAGTGGTGA AAAATCTGAA TCCATAAGCG 1801 TTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1861 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTCTTG 1921 GCTTTATATA TCTTGTGGAA AGGACGACGG GCTCGAAACT TATCCGTGGA CACGCGTTAA 1981 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t