Construct: ORF TRCN0000477425

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008872.1_s317c1
Derived from:
ccsbBroadEn_07949
DNA Barcode:
GGGGGGGACAGCACGACATCACTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MGAT4C (25834)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477425
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25834 MGAT4C MGAT4 family member C NM_001351285.2 99.9% 99.7% 1283C>G
2 human 25834 MGAT4C MGAT4 family member C NM_001351286.2 99.9% 99.7% 1283C>G
3 human 25834 MGAT4C MGAT4 family member C NM_001351287.2 99.9% 99.7% 1283C>G
4 human 25834 MGAT4C MGAT4 family member C NM_001351288.2 99.9% 99.7% 1283C>G
5 human 25834 MGAT4C MGAT4 family member C NM_001351289.2 99.9% 99.7% 1283C>G
6 human 25834 MGAT4C MGAT4 family member C NM_001351291.2 99.9% 99.7% 1283C>G
7 human 25834 MGAT4C MGAT4 family member C NM_013244.5 99.9% 99.7% 1283C>G
8 human 25834 MGAT4C MGAT4 family member C XM_024448932.1 99.3% 99.1% 1_9delATGAAGAGA;1292C>G
9 human 25834 MGAT4C MGAT4 family member C NM_001351283.2 94.2% 94% 1_87del;1370C>G
10 human 25834 MGAT4C MGAT4 family member C NM_001351284.2 94.2% 94% 1_87del;1370C>G
11 human 25834 MGAT4C MGAT4 family member C XM_017019146.2 94.2% 94% 1_87del;1370C>G
12 human 25834 MGAT4C MGAT4 family member C NM_001351282.2 92.5% 92.4% 1_114del;1397C>G
13 human 25834 MGAT4C MGAT4 family member C XM_011538151.3 92.5% 92.4% 1_114del;1397C>G
14 human 25834 MGAT4C MGAT4 family member C XM_017019144.2 92.5% 92.4% 1_114del;1397C>G
15 human 25834 MGAT4C MGAT4 family member C XM_024448931.1 92.5% 92.4% 1_114del;1397C>G
16 human 25834 MGAT4C MGAT4 family member C XM_017019143.2 90.6% 90.5% 1_147del;1430C>G
17 human 25834 MGAT4C MGAT4 family member C XM_024448930.1 86.5% 86.4% 1_222del;1505C>G
18 human 25834 MGAT4C MGAT4 family member C NR_147093.2 10.4% (many diffs)
19 mouse 67569 Mgat4c MGAT4 family, member C NM_001162368.1 88.9% 94.5% (many diffs)
20 mouse 67569 Mgat4c MGAT4 family, member C NM_001162369.1 88.9% 94.5% (many diffs)
21 mouse 67569 Mgat4c MGAT4 family, member C NM_001205098.1 88.9% 94.5% (many diffs)
22 mouse 67569 Mgat4c MGAT4 family, member C NM_026243.4 88.9% 94.5% (many diffs)
23 mouse 67569 Mgat4c MGAT4 family, member C XM_006514002.1 88.9% 94.5% (many diffs)
24 mouse 67569 Mgat4c MGAT4 family, member C XM_006514003.1 88.9% 94.5% (many diffs)
25 mouse 67569 Mgat4c MGAT4 family, member C XM_006514004.3 88.9% 94.5% (many diffs)
26 mouse 67569 Mgat4c MGAT4 family, member C XM_006514005.2 88.9% 94.5% (many diffs)
27 mouse 67569 Mgat4c MGAT4 family, member C XM_011243538.2 88.9% 94.5% (many diffs)
28 mouse 67569 Mgat4c MGAT4 family, member C XM_011243539.1 88.9% 94.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1503
ORF length:
1434
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtttaaattt catcaaatga aacatatttt tgaaatactt gataaaatga
121gatgcctgag aaaacgttct acagtgtcat tcttgggagt tcttgtcatt tttctccttt
181ttatgaactt gtacattgaa gatagctatg ttctggaagg agacaaacaa cttataaggg
241aaacatccac acatcaactg aattcagaac gctatgttca tactttcaag gatttatcta
301atttctcagg agccataaat gtcacctatc gctacctagc tgccacacct ttacaaagaa
361agcggtatct tacaattgga ctttcttcag taaagcgaaa aaaaggaaac tatttacttg
421agacaattaa gtcaattttt gagcaatcca gctatgaaga gctgaaggaa atttcagtgg
481tggttcacct agcagacttt aattcttcct ggcgtgatgc catggtccag gatattacac
541agaaatttgc gcaccatatt attgcaggaa gattaatggt tatacatgct ccagaggagt
601attacccaat cctagatggc cttaaaagaa attacaatga tccagaagat agagtcaaat
661ttcgttccaa gcaaaatgta gattatgctt ttctgcttaa tttttgtgcc aatacttcag
721actattatgt aatgcttgaa gatgatgttc gatgttcaaa aaatttctta actgccatca
781agaaagtcat tgcatcccta gaaggaactt actgggtaac tcttgaattc tctaagcttg
841gctacattgg taaactctat cattctcatg atctcccacg tttggcccat tttttattaa
901tgttttatca agaaatgcct tgtgattggc tattgactca tttccgtggt ctgttggctc
961agaaaaatgt gatccgtttt aaaccatctc tctttcagca catgggctat tattcatcat
1021acaaagggac ggagaataag ctgaaggatg atgattttga agaggagtca tttgacattc
1081ctgataaccc ccctgcaagt ctgtacacca acatgaatgt gtttgaaaat tatgaagcaa
1141gcaaggctta cagtagtgtt gatgagtact tttgggggaa acCACCTTCA ACAGGAGATG
1201TTTTTGTGAT TGTATTTGAA AATCCAATTA TAATAAAAAA AATTAAAGTA AATACTGGAA
1261CAGAAGATCG GCAAAATGAT ATTTTGCATC ATGGAGCCCT AGATGTTGGG GAAAACGTTA
1321TGCCTAGCAA ACAAAGGAGA CAATGTTCTA GTTACTTAAG ACTAGGAGAA TTCAAAAATG
1381GAAACTTTGA AATGTCAGGT GTAAATCAAA AAATTCCATT TGATATACAT TGTATGAGGA
1441TATATGTCAC CAAAACACAA AAGGAATGGC TAATTATTAG GAGTATTAGC ATTTGGACTT
1501CTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1561TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1621GGCTTTATAT ATCTTGTGGA AAGGACGAGG GGGGGACAGC ACGACATCAC TGACGCGTTA
1681AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)