Construct: ORF TRCN0000477433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016477.1_s317c1
- Derived from:
- ccsbBroadEn_13366
- DNA Barcode:
- AGCACGCCCTCCCTGCCATATACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLPP4 (196051)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477433
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | NM_001318167.1 | 100% | 100% | |
| 2 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_005269592.2 | 82% | 71.7% | (many diffs) |
| 3 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | NM_001030059.2 | 76.7% | 76.3% | 257_445del |
| 4 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | NM_001318166.1 | 74.8% | 48% | 255_318del;579_580ins109 |
| 5 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_006717685.4 | 71.4% | 69.3% | (many diffs) |
| 6 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_011539444.3 | 71.2% | 67.6% | (many diffs) |
| 7 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_017015821.2 | 66.8% | 55.8% | (many diffs) |
| 8 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_017015822.1 | 66.8% | 55.8% | (many diffs) |
| 9 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_024447860.1 | 66.8% | 55.8% | (many diffs) |
| 10 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_024447861.1 | 66.8% | 55.8% | (many diffs) |
| 11 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_024447862.1 | 66.8% | 55.8% | (many diffs) |
| 12 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_011539445.2 | 59.1% | 53.7% | (many diffs) |
| 13 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_006717686.4 | 56.8% | 52.3% | (many diffs) |
| 14 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_017015820.1 | 53.9% | 46.9% | (many diffs) |
| 15 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | NM_001318169.1 | 46.1% | 33.1% | 165_166ins262;288_289ins74 |
| 16 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | NM_001318168.1 | 46.1% | 30.2% | 165_166ins91;288_289ins245 |
| 17 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_017015823.1 | 46.1% | 30.2% | 165_166ins91;288_289ins245 |
| 18 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | XM_017015824.2 | 46.1% | 30.2% | 165_166ins91;288_289ins245 |
| 19 | human | 196051 | PLPP4 | phospholipid phosphatase 4 | NR_134516.1 | 45.8% | (many diffs) | |
| 20 | mouse | 381925 | Plpp4 | phospholipid phosphatase 4 | XM_017322324.1 | 91.3% | 99.5% | (many diffs) |
| 21 | mouse | 381925 | Plpp4 | phospholipid phosphatase 4 | XM_017322323.1 | 70.4% | 76.6% | (many diffs) |
| 22 | mouse | 381925 | Plpp4 | phospholipid phosphatase 4 | NM_001080963.1 | 70.1% | 76% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 693
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgggagctg gccattgaga tcggggtgcg agccctgctc ttcggagtct 121 tcgtttttac agagtttttg gatccgttcc agagagtcat ccagccagaa gagatctggc 181 tctataaaaa tcctttggtg caatcagata acatacctac ccgcctcatg tttgcaattt 241 ctttcctcac acccctggct gttatttgtg tggtgaaaat tatccggcga acagacaaga 301 ctgaaattaa ggaagccttc ttagttgcct tttcgggcct tGGCTTCACG ACGTTCTACT 361 TGGCGGGCAA GCTGCACTGC TTCACCGAGA GTGGGCGGGG AAAGAGCTGG CGGCTCTGTG 421 CTGCCATCCT GCCCTTGTAC TGCGCCATGA TGATTGCCCT GTCCCGCATG TGCGACTACA 481 AGCATCACTG GCAAGATTCC TTTGTGGGTG GAGTCATCGG CCTCATTTTT GCATACATTT 541 GCTACAGACA GCACTATCCT CCTCTGGCCA ACACAGCTTG CCATAAACCC TACGTTAGTC 601 TGCGAGTCCC AGCCTCACTG AAGAAAGAGG AGAGGCCCAC AGCTGACAGC GCACCCAGCT 661 TGCCTCTGGA GGGGATCACC GAAGGCCCGG TATTGCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 841 CACGCCCTCC CTGCCATATA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt