Transcript: Human XM_017015822.1

PREDICTED: Homo sapiens phospholipid phosphatase 4 (PLPP4), transcript variant X10, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PLPP4 (196051)
Length:
11166
CDS:
93..545

Additional Resources:

NCBI RefSeq record:
XM_017015822.1
NBCI Gene record:
PLPP4 (196051)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017015822.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360271 TGGAGTGATGAACTCGGAAAT pLKO_005 86 5UTR 100% 10.800 15.120 N PLPP4 n/a
2 TRCN0000052733 GCTTGCCATAAACCCTACGTT pLKO.1 426 CDS 100% 3.000 4.200 N PLPP4 n/a
3 TRCN0000052736 CATTTGCTACAGACAGCACTA pLKO.1 386 CDS 100% 4.050 2.835 N PLPP4 n/a
4 TRCN0000072628 CCTCCCAAAGTGCTAGGATTA pLKO.1 6236 3UTR 100% 10.800 5.400 Y MRPS16 n/a
5 TRCN0000178912 CCGGAATTTGAATCCTGCAAT pLKO.1 10622 3UTR 100% 4.950 2.475 Y LINC01561 n/a
6 TRCN0000179518 GAATCTTGAGTGTCCAGATCA pLKO.1 9556 3UTR 100% 4.950 2.475 Y LINC01561 n/a
7 TRCN0000183438 GCAGTGAATTTGGTTGTTCTT pLKO.1 10689 3UTR 100% 4.950 2.475 Y LINC01561 n/a
8 TRCN0000140657 CCTCCCAAAGTGCTAGGATAA pLKO.1 6236 3UTR 100% 10.800 5.400 Y CD3EAP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017015822.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13366 pDONR223 100% 66.8% 55.8% None (many diffs) n/a
2 ccsbBroad304_13366 pLX_304 0% 66.8% 55.8% V5 (many diffs) n/a
3 TRCN0000477433 AGCACGCCCTCCCTGCCATATACC pLX_317 55.9% 66.8% 55.8% V5 (many diffs) n/a
4 ccsbBroadEn_05169 pDONR223 100% 55.3% 55.3% None 0_1ins363 n/a
5 ccsbBroad304_05169 pLX_304 0% 55.3% 55.3% V5 0_1ins363 n/a
6 TRCN0000469398 ATGGCTGAAACGTTTTGAGTCATT pLX_317 43.3% 55.3% 55.3% V5 0_1ins363 n/a
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