Transcript: Mouse XM_017322324.1

PREDICTED: Mus musculus phospholipid phosphatase 4 (Plpp4), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Plpp4 (381925)
Length:
1078
CDS:
95..721

Additional Resources:

NCBI RefSeq record:
XM_017322324.1
NBCI Gene record:
Plpp4 (381925)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017322324.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000080691 GCCTATATTTGCTACAGACAA pLKO.1 557 CDS 100% 4.950 6.930 N Plpp4 n/a
2 TRCN0000360201 GAAGAGATCTGGCTCTATAAA pLKO_005 194 CDS 100% 15.000 10.500 N PLPP4 n/a
3 TRCN0000052735 CCCGCCTCATGTTTGCAATTT pLKO.1 246 CDS 100% 13.200 9.240 N PLPP4 n/a
4 TRCN0000080689 CCTCGGTTTCACAACATTCTA pLKO.1 364 CDS 100% 5.625 3.938 N Plpp4 n/a
5 TRCN0000080692 GCCTCATGTTTGCAATTTCTT pLKO.1 249 CDS 100% 5.625 3.938 N Plpp4 n/a
6 TRCN0000080688 CGTTCTGTGGATGGTGTTCTT pLKO.1 846 3UTR 100% 4.950 3.465 N Plpp4 n/a
7 TRCN0000080690 GCTGTTATTTGCGTGGTGAAA pLKO.1 284 CDS 100% 4.950 3.465 N Plpp4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017322324.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13366 pDONR223 100% 91.3% 99.5% None (many diffs) n/a
2 ccsbBroad304_13366 pLX_304 0% 91.3% 99.5% V5 (many diffs) n/a
3 TRCN0000477433 AGCACGCCCTCCCTGCCATATACC pLX_317 55.9% 91.3% 99.5% V5 (many diffs) n/a
4 ccsbBroadEn_05169 pDONR223 100% 70.1% 76% None (many diffs) n/a
5 ccsbBroad304_05169 pLX_304 0% 70.1% 76% V5 (many diffs) n/a
6 TRCN0000469398 ATGGCTGAAACGTTTTGAGTCATT pLX_317 43.3% 70.1% 76% V5 (many diffs) n/a
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