Construct: ORF TRCN0000477451

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010275.1_s317c1
Derived from:
ccsbBroadEn_00448
DNA Barcode:
GAAAAATTTTCGGACATAACTCAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DLG3 (1741)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477451
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1741 DLG3 discs large MAGUK scaffold ... NM_021120.4 100% 100%
2 human 1741 DLG3 discs large MAGUK scaffold ... XM_006724626.2 98.3% 98.3% 1819_1860del
3 human 1741 DLG3 discs large MAGUK scaffold ... XM_011530883.1 97.2% 95.9% (many diffs)
4 human 1741 DLG3 discs large MAGUK scaffold ... XM_006724625.2 95.6% 94.3% (many diffs)
5 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029324.2 56.9% 54.3% (many diffs)
6 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029323.2 56% 53.4% (many diffs)
7 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029322.2 55.1% 51.5% (many diffs)
8 human 1741 DLG3 discs large MAGUK scaffold ... NM_020730.3 54.3% 50.7% (many diffs)
9 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029327.1 40.8% 40.8% 0_1ins1449
10 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029325.2 40.6% 39.5% (many diffs)
11 human 1741 DLG3 discs large MAGUK scaffold ... NM_001166278.1 38.8% 37.8% (many diffs)
12 human 1741 DLG3 discs large MAGUK scaffold ... XM_005262248.4 38.8% 37.8% (many diffs)
13 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029326.2 38.8% 37.8% (many diffs)
14 mouse 53310 Dlg3 discs, large homolog 3 (Dro... NM_001290402.1 93% 99.2% (many diffs)
15 mouse 53310 Dlg3 discs, large homolog 3 (Dro... NM_001177778.2 91% 97.1% (many diffs)
16 mouse 53310 Dlg3 discs, large homolog 3 (Dro... NM_016747.4 89.5% 95.5% (many diffs)
17 mouse 53310 Dlg3 discs, large homolog 3 (Dro... XM_006528124.3 89% 93.6% (many diffs)
18 mouse 53310 Dlg3 discs, large homolog 3 (Dro... XM_006528123.3 87.2% 91.7% (many diffs)
19 mouse 53310 Dlg3 discs, large homolog 3 (Dro... NM_001177779.1 51.4% 50.7% (many diffs)
20 mouse 53310 Dlg3 discs, large homolog 3 (Dro... XR_001782754.1 42.8% (many diffs)
21 mouse 53310 Dlg3 discs, large homolog 3 (Dro... NM_001177780.1 36.5% 37.7% (many diffs)
22 mouse 53310 Dlg3 discs, large homolog 3 (Dro... XM_006528127.3 36.5% 37.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2520
ORF length:
2451
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcgccat gcacaagcac cagcactgct gtaagtgccc tgagtgctat gaggtgaccc
121gcctggccgc cctgcggcgc ctcgagcctc cgggctacgg cgactggcaa gtccccgacc
181cttacgggcc aggtgggggc aacggcgcca gcgcgggtta tgggggctac agctcgcaga
241ccttgccctc gcaggcgggg gccaccccca cccctcgcac caaggccaag ctcatcccca
301ccggccggga tgtggggccg gtgcctccta agccagtccc gggcaagagc acccccaaac
361tcaacggcag cggccccagc tggtggccag agtgcacctg taccaaccgg gactggtatg
421agcaggtgaa tggcagtgat ggcatgttca aatatgagga aatcgtactt gagaggggca
481actctggcct gggcttcagt atcgcaggtg gcatcgacaa tccccatgtc cctgatgacc
541ctggcatctt tattaccaag attatccctg gtggagcagc tgccatggat gggaggctgg
601gggtgaatga ctgtgtgctg cgggtgaatg aggtggacgt gtcggaggtg gtacacagcc
661gggcggtgga ggcgctgaag gaggcaggcc ctgtggtgcg attggtggtg cggaggcgac
721agcctccacc cgagaccatc atggaggtca acctgctcaa agggcccaaa ggcctgggtt
781tcagcattgc tgggggtatt ggcaaccagc acatcccagg agacaacagc atctacatca
841ccaagatcat tgaggggggt gctgctcaga aggatggacg cctacagatt ggggaccggc
901tgctggcggt gaacaacacc aatctgcagg atgtgaggca cgaggaagct gtggcctcac
961tgaagaacac atctgatatg gtgtatttga aggtggccaa gccaggcagc ctccacctca
1021acgacatgta cgctccccct gactacgcca gcacttttac tgccttggct gacaaccaca
1081taagccataa ttccagcctg ggttatctcg gggctgtgga gagcaaggtc agctaccctg
1141ctcctcctca ggttcccccc acccgctact ctcctattcc caggcacatg ctggctgagg
1201aggacttcac cagagagcct cgcaagatca tcctgcacaa aggctccaca ggcctgggct
1261tcaacatcgt aggaggagag gatggagaag gcatttttgt ctccttcatc ctggcaggag
1321gcccagctga cctgagtggg gagctgcgca ggggagaccg gatcttatcg gtgaatggag
1381tgaatctgag gaatgcaact catgagcagg ctgcagctgc tctgaaacgg gccggccagt
1441cagtcaccat tgtggcccag tacagacctg aagaatacag tcgctttgaa tcgaagatac
1501atgacttacg agaacaaatg atgaacagca gcatgagctc tgggtctggg tccctccgaa
1561caagtgaaaa gaggtccttg tatgtcaggg ccctgtttga ttatgatcgg actcgggaca
1621gctgcctgcc aagccagggg ctcagcttct cttatggtga cattctgcat gtcattaatg
1681cctctgatga tgagtggtgg caggcaaggc tggtgacccc acacggagaa agtgagcaga
1741tcggtgtgat ccccagtaag aagagggtgg aaaagaaaga aagagctcga ttgaaaactg
1801tgaagttcca tgccaggacg gggatgattg agtctaacag ggacttcccg gggttaagtg
1861acgattatta tggagcaaag aacctgaaag gacaagagga tgctattttg tcatatgagc
1921cagtgacacg gcaagaaatt cactatgcaa ggcctgtgat catcctgggc ccaatgaagg
1981accgagtcaa tgatgacctg atctccgaat ttccacataa atttggatcc tgtgtgccac
2041atactacccg gcctcGACGT GATAATGAGG TGGATGGACA AGACTACCAC TTTGTGGTGT
2101CCCGAGAACA AATGGAGAAA GATATTCAGG ACAACAAGTT CATCGAGGCG GGCCAATTTA
2161ATGATAACCT CTATGGGACC AGCATCCAGT CAGTGCGGGC AGTTGCAGAG AGGGGCAAGC
2221ACTGCATCTT AGATGTTTCC GGCAATGCTA TCAAGAGACT GCAGCAAGCA CAACTTTACC
2281CCATTGCCAT TTTCATCAAG CCCAAGTCCA TTGAAGCCCT TATGGAAATG AACCGAAGGC
2341AGACATATGA ACAAGCAAAT AAGATCTATG ACAAAGCCAT GAAACTGGAG CAGGAATTTG
2401GAGAGTACTT TACAGCCATT GTACAGGGTG ACTCACTGGA AGAGATTTAT AACAAAATCA
2461AACAAATCAT TGAGGACCAG TCTGGGCACT ACATTTGGGT CCCATCCCCT GAAAAACTCT
2521TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
2581GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2641TTTATATATC TTGTGGAAAG GACGAGAAAA ATTTTCGGAC ATAACTCATA CGCGTTAAGT
2701Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)