Transcript: Human XM_006724625.2

PREDICTED: Homo sapiens discs large MAGUK scaffold protein 3 (DLG3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DLG3 (1741)
Length:
6123
CDS:
353..2902

Additional Resources:

NCBI RefSeq record:
XM_006724625.2
NBCI Gene record:
DLG3 (1741)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147306 GGGGATGATTGAGTCTAACA pXPR_003 GGG 1768 69% 12 0.8615 DLG3 DLG3 76156
2 BRDN0001145728 TGTCATATGAGCCAGTGACA pXPR_003 CGG 1953 77% 15 0.7266 DLG3 DLG3 76157
3 BRDN0001149280 AGTCCGATCATAATCAAACA pXPR_003 GGG 1528 60% 11 0.3917 DLG3 DLG3 76158
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006724625.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000219897 TGGCGACAGAAAGTATCTTAT pLKO.1 3369 3UTR 100% 13.200 18.480 N DLG3 n/a
2 TRCN0000140857 CCAATGAAGGACCGAGTCAAT pLKO.1 2351 CDS 100% 4.950 6.930 N DLG3 n/a
3 TRCN0000330032 CCAATGAAGGACCGAGTCAAT pLKO_005 2351 CDS 100% 4.950 6.930 N DLG3 n/a
4 TRCN0000140913 CCGAGTCAATGATGACCTGAT pLKO.1 2362 CDS 100% 4.050 5.670 N DLG3 n/a
5 TRCN0000219896 ACACATCTGATATGGTGTATT pLKO.1 1251 CDS 100% 13.200 10.560 N DLG3 n/a
6 TRCN0000139121 CGACGTGATAATGAGGTGGAT pLKO.1 2435 CDS 100% 2.640 2.112 N DLG3 n/a
7 TRCN0000144685 GACTCACTGGAAGAGATTTAT pLKO.1 2810 CDS 100% 15.000 10.500 N DLG3 n/a
8 TRCN0000330033 GACTCACTGGAAGAGATTTAT pLKO_005 2810 CDS 100% 15.000 10.500 N DLG3 n/a
9 TRCN0000144753 GAGTACTTTACAGCCATTGTA pLKO.1 2783 CDS 100% 5.625 3.938 N DLG3 n/a
10 TRCN0000330107 GAGTACTTTACAGCCATTGTA pLKO_005 2783 CDS 100% 5.625 3.938 N DLG3 n/a
11 TRCN0000024848 CGCAAGATCATCCTGCACAAA pLKO.1 1505 CDS 100% 4.950 3.465 N Dlg3 n/a
12 TRCN0000140912 CGCAAGATCATCCTGCACAAA pLKO.1 1505 CDS 100% 4.950 3.465 N DLG3 n/a
13 TRCN0000330105 CGCAAGATCATCCTGCACAAA pLKO_005 1505 CDS 100% 4.950 3.465 N DLG3 n/a
14 TRCN0000139153 CATCATGTGACTGTGCCCATT pLKO.1 3119 3UTR 100% 4.050 2.835 N DLG3 n/a
15 TRCN0000139338 CCAAGTCCATTGAAGCCCTTA pLKO.1 2682 CDS 100% 4.050 2.835 N DLG3 n/a
16 TRCN0000330034 GGCTTGTGAAGTGAGCTAAAT pLKO_005 3270 3UTR 100% 13.200 7.920 N DLG3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006724625.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00448 pDONR223 100% 95.6% 94.3% None (many diffs) n/a
2 ccsbBroad304_00448 pLX_304 0% 95.6% 94.3% V5 (many diffs) n/a
3 TRCN0000477451 GAAAAATTTTCGGACATAACTCAT pLX_317 19.1% 95.6% 94.3% V5 (many diffs) n/a
4 ccsbBroadEn_14616 pDONR223 100% 57.3% 22.2% None (many diffs) n/a
5 ccsbBroad304_14616 pLX_304 0% 57.3% 22.2% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000474898 GCATCTGGCTCATCTTTTACCAAT pLX_317 30.4% 57.3% 22.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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