Construct: ORF TRCN0000477457

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010625.1_s317c1
Derived from:
ccsbBroadEn_07601
DNA Barcode:
ACATGTTCCTATACTGTTACCAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
YAP1 (10413)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477457
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10413 YAP1 Yes associated protein 1 NM_001130145.3 99.8% 99.8% 1491A>C;1510T>C
2 human 10413 YAP1 Yes associated protein 1 XM_005271380.3 99.4% 99.4% 802_807delGGTAAA;1497A>C;1516T>C
3 human 10413 YAP1 Yes associated protein 1 NM_001282101.1 99% 99% 982_993del;1503A>C;1522T>C
4 human 10413 YAP1 Yes associated protein 1 XM_005271378.3 98.6% 98.6% (many diffs)
5 human 10413 YAP1 Yes associated protein 1 NM_001282100.1 97.4% 97% (many diffs)
6 human 10413 YAP1 Yes associated protein 1 XM_005271381.3 97% 96.6% (many diffs)
7 human 10413 YAP1 Yes associated protein 1 NM_001195044.2 96.6% 96.6% 981_982ins48;1443A>C;1462T>C
8 human 10413 YAP1 Yes associated protein 1 XM_005271383.3 96.3% 96.2% (many diffs)
9 human 10413 YAP1 Yes associated protein 1 NM_001282097.2 92.3% 92.2% 687_688ins114;1377A>C;1396T>C
10 human 10413 YAP1 Yes associated protein 1 NM_001282099.1 91.6% 91.5% (many diffs)
11 human 10413 YAP1 Yes associated protein 1 NM_001282098.2 89.8% 89.4% (many diffs)
12 human 10413 YAP1 Yes associated protein 1 NM_006106.5 89.1% 89% (many diffs)
13 human 10413 YAP1 Yes associated protein 1 NM_001195045.2 64.5% 64.4% 0_1ins534;957A>C;976T>C
14 human 10413 YAP1 Yes associated protein 1 XM_011542556.2 64% 63.9% (many diffs)
15 human 10413 YAP1 Yes associated protein 1 XM_011542555.2 63.7% 63.7% (many diffs)
16 human 10413 YAP1 Yes associated protein 1 XM_017017093.1 61.3% 61.3% (many diffs)
17 mouse 22601 Yap1 yes-associated protein 1 NM_001171147.1 85.5% 88.4% (many diffs)
18 mouse 22601 Yap1 yes-associated protein 1 XM_006509854.3 85.1% 88.1% (many diffs)
19 mouse 22601 Yap1 yes-associated protein 1 XM_006509853.3 84.8% 87.7% (many diffs)
20 mouse 22601 Yap1 yes-associated protein 1 XM_006509852.2 84.5% 87.4% (many diffs)
21 mouse 22601 Yap1 yes-associated protein 1 XM_006509856.3 83.1% 86.1% (many diffs)
22 mouse 22601 Yap1 yes-associated protein 1 XM_006509855.3 82.8% 85.7% (many diffs)
23 mouse 22601 Yap1 yes-associated protein 1 NM_009534.3 82.4% 85.7% (many diffs)
24 mouse 22601 Yap1 yes-associated protein 1 XM_006509857.3 82.1% 85.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1578
ORF length:
1512
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tcccgggcag cagccgccgc ctcaaccggc cccccagggc caagggcagc
121cgccttcgca gcccccgcag gggcagggcc cgccgtccgg acccgggcaa ccggcacccg
181cggcgaccca ggcggcgccg caggcacccc ccgccgggca tcagatcgtg cacgtccgcg
241gggactcgga gaccgacctg gaggcgctct tcaacgccgt catgaacccc aagacggcca
301acgtgcccca gaccgtgccc atgaggctcc ggaagctgcc cgactccttc ttcaagccgc
361cggagcccaa atcccactcc cgacaggcca gtactgatgc aggcactgca ggagccctga
421ctccacagca tgttcgagct cattcctctc cagcttctct gcagttggga gctgtttctc
481ctgggacact gacccccact ggagtagtct ctggcccagc agctacaccc acagctcagc
541atcttcgaca gtcttctttt gagatacctg atgatgtacc tctgccagca ggttgggaga
601tggcaaagac atcttctggt cagagatact tcttaaatca catcgatcag acaacaacat
661ggcaggaccc caggaaggcc atgctgtccc agatgaacgt cacagccccc accagtccac
721cagtgcagca gaatatgatg aactcggctt caggtcctct tcctgatgga tgggaacaag
781ccatgactca ggatggagaa atttactata taaaccataa gaacaagacc acctcttggc
841tagacccaag gcttgaccct cgttttgcca tgaaccagag aatcagtcag agtgctccag
901tgaaacagcc accacccctg gctccccaga gcccacaggg aggcgtcatg ggtggcagca
961actccaacca gcagcaacag atgcgactgc agcaactgca gatggagaag gagaggctgc
1021ggctgaaaca gcaagaactg cttcggcagg caatgcggaa tatcaatccc agcacagcaa
1081attctccaaa atgtcaggag ttagccctgc gtagccagtt accaacactg gagcaggatg
1141gtgggactca aaatccagtg tcttctcccg ggatgtctca ggaattgaga acaatgacga
1201ccaatagctc agatcctttc cttaacagtg gcacctatca ctctcgagat gagagtacag
1261acagtggact aagcatgagc agcTACAGTG TCCCTCGAAC CCCAGATGAC TTCCTGAACA
1321GTGTGGATGA GATGGATACA GGTGATACTA TCAACCAAAG CACCCTGCCC TCACAGCAGA
1381ACCGTTTCCC AGACTACCTT GAAGCCATTC CTGGGACAAA TGTGGACCTT GGAACACTGG
1441AAGGAGATGG AATGAACATA GAAGGAGAGG AGCTGATGCC AAGTCTGCAG GAAGCTTTGA
1501GTTCTGACAT CCTTAATGAC ATGGAGTCTG TTTTGGCTGC CACCAAGCTA GATAACGAAA
1561GCTTTCTTAC ATGGCTATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1621TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1681GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACATGTT CCTATACTGT
1741TACCAATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)