Construct: ORF TRCN0000477463

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002725.1_s317c1
Derived from:
ccsbBroadEn_04340
DNA Barcode:
TTTGTTTTACTGATTTTTACTAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM164 (84187)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477463
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84187 TMEM164 transmembrane protein 164 NM_001353849.2 100% 100%
2 human 84187 TMEM164 transmembrane protein 164 NM_032227.4 100% 100%
3 human 84187 TMEM164 transmembrane protein 164 XM_005262205.4 100% 100%
4 human 84187 TMEM164 transmembrane protein 164 XM_005262208.5 61.6% 53.6% (many diffs)
5 human 84187 TMEM164 transmembrane protein 164 XM_017029894.2 58.8% 56.5% (many diffs)
6 human 84187 TMEM164 transmembrane protein 164 NM_001353850.2 57.2% 57.2% 0_1ins381
7 human 84187 TMEM164 transmembrane protein 164 NM_001353851.1 57.2% 57.2% 0_1ins381
8 human 84187 TMEM164 transmembrane protein 164 XM_017029899.1 57.2% 57.2% 0_1ins381
9 human 84187 TMEM164 transmembrane protein 164 XM_017029897.1 56.5% 56.2% 0_1ins384;3_4insCAT
10 human 84187 TMEM164 transmembrane protein 164 XM_017029895.1 53.2% 42.7% (many diffs)
11 human 84187 TMEM164 transmembrane protein 164 XM_017029898.1 52.1% 42.8% 390_391ins50;465_466ins376
12 human 84187 TMEM164 transmembrane protein 164 XM_017029900.1 50.7% 50.5% 0_1ins438;2T>A
13 human 84187 TMEM164 transmembrane protein 164 NM_017698.2 49.8% 49.8% 0_1ins447
14 human 84187 TMEM164 transmembrane protein 164 XM_011531054.1 49.8% 49.8% 0_1ins447
15 human 84187 TMEM164 transmembrane protein 164 XM_011531055.2 49.8% 49.8% 0_1ins447
16 mouse 209497 Tmem164 transmembrane protein 164 NM_001199360.1 95.6% 94.9% (many diffs)
17 mouse 209497 Tmem164 transmembrane protein 164 NM_177592.4 95.6% 94.9% (many diffs)
18 mouse 209497 Tmem164 transmembrane protein 164 XM_006528779.1 83.7% 82.8% (many diffs)
19 mouse 209497 Tmem164 transmembrane protein 164 XM_006528780.1 82.6% 73.8% (many diffs)
20 mouse 209497 Tmem164 transmembrane protein 164 XM_006528781.1 65.7% 57.3% (many diffs)
21 mouse 209497 Tmem164 transmembrane protein 164 XM_011247804.1 57.6% 52.6% (many diffs)
22 mouse 209497 Tmem164 transmembrane protein 164 NM_001199357.1 47.6% 47.8% (many diffs)
23 mouse 209497 Tmem164 transmembrane protein 164 XM_006528783.3 47.6% 47.8% (many diffs)
24 mouse 209497 Tmem164 transmembrane protein 164 XM_006528784.2 47.6% 47.8% (many diffs)
25 mouse 209497 Tmem164 transmembrane protein 164 XM_006528785.1 47.6% 47.8% (many diffs)
26 mouse 209497 Tmem164 transmembrane protein 164 XM_006528786.3 47.6% 47.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
960
ORF length:
891
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtcccggtat agctaccaga gtctcctgga ctggctctat gggggcgtgg
121accccagttt tgcaggcaat gggggccccg actgtgctgc cttcctctct tggcagcagc
181ggctgctgga aagtgtggtg gtcctgaccc tggctctgtt ggagatcctg gtggccctgc
241ggcacatcct gaggcagacg aaggaggacg gtaggggtag ccctggcagc cagccagagc
301aggtgaccca gcggccagag gaaggcaagg agagcctgag caagaatctg ctcttagtag
361ccctgtgcct gaccttcggg gtggaggtgg gctttaagtt cgccaccaag accgtcatct
421acctgctcaa cccctgtcac ctggtcacca tgatgcatat ctttctcctg gcctgccctc
481catgtcgggg agctatcgtc gtcttcaagc tacagatgca catgttgaat ggagctcTTC
541TGGCATTGCT GTTTCCTGTG GTAAACACTC GGCTGCTCCC CTTTGAATTG GAGATTTACT
601ACATTCAGCA TGTTATGCTC TACGTGGTAC CCATCTACCT GCTTTGGAAA GGAGGTGCTT
661ACACTCCAGA GCCCCTCAGC AGTTTCCGGT GGGCTCTTCT CTCAACTGGC CTCATGTTCT
721TTTATCACTT CAGCGTCTTG CAGATCCTCG GCCTGGTCAC CGAAGTGAAT TTGAACAACA
781TGCTGTGTCC GGCCATCTCA GACCCATTCT ACGGCCCCTG GTATCGCATC TGGGCCTCGG
841GACACCAGAC TCTCATGACC ATGACCCACG GGAAGCTGGT CATCCTGTTC TCATACATGG
901CTGGGCCCTT GTGTAAATAT CTGCTGGATT TGCTCCGGCT TCCAGCCAAG AAAATAGACT
961TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1021GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1081TTTATATATC TTGTGGAAAG GACGATTTGT TTTACTGATT TTTACTAATA CGCGTTAAGT
1141Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)