Construct: ORF TRCN0000477479

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013744.1_s317c1
Derived from:
ccsbBroadEn_13268
DNA Barcode:
GCGGCTGATGGCCGATATAAGTCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEPTIN10 (151011)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477479
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 151011 SEPTIN10 septin 10 XM_006712317.2 95% 92.6% (many diffs)
2 human 151011 SEPTIN10 septin 10 NM_001321498.2 93% 92.2% 1497_1498insA;1521_1632del
3 human 151011 SEPTIN10 septin 10 XM_011510703.2 91.3% 90.4% 1_1delAins28;1470_1471insA;1494_1605del
4 human 151011 SEPTIN10 septin 10 XM_011510698.2 90.3% 89.5% 0_1ins45;1452_1453insA;1476_1587del
5 human 151011 SEPTIN10 septin 10 NM_144710.5 89.5% 88.5% 1349_1350ins83;1362_1363ins76
6 human 151011 SEPTIN10 septin 10 XM_011510699.2 88.8% 88% 30_31ins69;1428_1429insA;1452_1563del
7 human 151011 SEPTIN10 septin 10 NM_001321512.2 88.7% 88.7% 1350_1351ins171
8 human 151011 SEPTIN10 septin 10 NM_001321513.2 86.9% 86.7% 1_1delAins28;1323_1324ins171
9 human 151011 SEPTIN10 septin 10 NM_001321514.2 86.5% 85.6% 0_1ins45;1304_1305ins83;1317_1318ins76
10 human 151011 SEPTIN10 septin 10 XM_011510700.2 85.3% 84.5% 0_1ins126;1371_1372insA;1395_1506del
11 human 151011 SEPTIN10 septin 10 NM_178584.4 85% 84% 30_31ins69;1280_1281ins83;1293_1294ins76
12 human 151011 SEPTIN10 septin 10 NM_001321500.2 84.2% 84.2% 30_31ins69;1281_1282ins171
13 human 151011 SEPTIN10 septin 10 NM_001321515.2 83.1% 82% (many diffs)
14 human 151011 SEPTIN10 septin 10 NM_001321509.2 81.2% 80.2% 0_1ins126;1223_1224ins83;1236_1237ins76
15 human 151011 SEPTIN10 septin 10 NM_001321501.2 80.4% 80.4% 0_1ins126;1224_1225ins171
16 human 151011 SEPTIN10 septin 10 XM_011510704.3 70.8% 68.2% 1026_1027ins133;1077_1078ins311
17 human 151011 SEPTIN10 septin 10 XM_011510701.3 68.2% 67% (many diffs)
18 human 151011 SEPTIN10 septin 10 NM_001321502.2 66.2% 63.7% 30_31ins69;957_958ins133;1008_1009ins311
19 human 151011 SEPTIN10 septin 10 NM_001321496.2 62.9% 61.5% (many diffs)
20 human 151011 SEPTIN10 septin 10 XM_011510702.2 62.4% 61.5% 99_100ins501;996_997insA;1020_1131del
21 human 151011 SEPTIN10 septin 10 NM_001321507.2 62.1% 61.7% (many diffs)
22 human 151011 SEPTIN10 septin 10 NM_001321510.2 56.6% 55.6% 99_100ins501;848_849ins83;861_862ins76
23 human 151011 SEPTIN10 septin 10 NM_001321499.2 55.8% 55.8% 99_100ins501;849_850ins171
24 human 151011 SEPTIN10 septin 10 NM_001321505.2 51.4% 50.4% 0_1ins579;770_771ins83;783_784ins76
25 human 151011 SEPTIN10 septin 10 NM_001321506.2 51.4% 49.1% (many diffs)
26 human 151011 SEPTIN10 septin 10 NM_001321504.2 50.6% 50.6% 0_1ins579;771_772ins171
27 human 151011 SEPTIN10 septin 10 NM_001321511.2 50.6% 49.3% (many diffs)
28 human 151011 SEPTIN10 septin 10 XR_922870.3 39% (many diffs)
29 human 151011 SEPTIN10 septin 10 NM_001321508.2 32.7% 30.1% 0_1ins579;447_448ins133;498_499ins311
30 human 151011 SEPTIN10 septin 10 NM_001321503.2 32.7% 28.7% (many diffs)
31 mouse 103080 Sept10 septin 10 XM_011243255.2 73.2% 71.3% (many diffs)
32 mouse 103080 Sept10 septin 10 NM_001024911.2 72.5% 71.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1590
ORF length:
1521
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcctcctcc gaggtggcgc ggcacctgct ctttcagtct cacatggcaa
121cgaaaacaac ttgtatgtct tcacaaggat cagatgatga acagataaaa agagaaaaca
181ttcgttcgtt gactatgtct ggccatgttg gttttgagag tttgcctgat cagctggtga
241acagatccat tcagcaaggt ttctgcttta atattctctg tgtgggggaa actggaattg
301gaaaatcaac actgattgac acattgttta atactaattt tgaagactat gaatcctcac
361atttttgccc aaatgttaaa cttaaagctc agacatatga actccaggaa agtaatgttc
421aattgaaatt gaccattgtg aatacagtgg gatttggtga ccaaataaat aaagaagaga
481gctaccaacc aatagttgac tacatagatg ctcagtttga ggcctatctc caagaagaac
541tgaagattaa gcgttctctc tttacctacc atgattctcg catccatgtg tgtctctact
601tcatttcacc gacaggccac tctctgaaga cacttgatct cttaaccatg aagaaccttg
661acagcaaggt aaacattata ccagtgattg ccaaagcaga tacggtttct aaaactgaat
721tacagaagtt taagatcaag ctcatgagtg aattggtcag caatggcgtc cagatatacc
781agttcccaac ggatgatgac actattgcta aggtcaacgc tgcaatgaat ggacagttgc
841cgtttgctgt tgtgggaagt atggatgagg taaaagtcgg aaacaagatg gtcaaagctc
901gccagtaccc ttggggtgtt gtacaagtgg aaaatgaaaa ccactgtgac tttgtaaagc
961tgcgggaaat gctcatttgt acaaatatgg aggacctgcg agagcagacc cataccaggc
1021actatgagct ttacaggcgc tgcaaactgg aggaaatggg ctttacagat gtgggcccag
1081aaaacaagcc agtcagtgtt caagagaccT ATGAAGCCAA AAGACATGAG TTCCATGGTG
1141AACGTCAGAG GAAGGAAGAA GAAATGAAAC AGATGTTTGT GCAGCGAGTA AAGGAGAAAG
1201AAGCCATATT GAAAGAAGCT GAGAGAGAGC TACAGGCCAA ATTTGAGCAC CTTAAGAGAC
1261TTCACCAAGA AGAGAGAATG AAGCTTGAAG AAAAGAGAAG ACTTTTGGAA GAAGAAATAA
1321TTGCTTTCTC TAAAAAGAAA GCTACCTCCG AGATATTTCA CAGCCAGTCC TTTCTGGCAA
1381CAGGCAGCAA CCTGAGGAAG GACAAGGACC GTAAGAAGGA ACCAGGCTGT CGATTCGAAC
1441TCCTGTGCAT TGATGTCAGA GCTTGTGAAA CCAATGGTGG ACGTAAAGAC GCAGAGAAAG
1501CTCCAATTTT TTGTAAAACA GAAGTTCCAG AGCACAGAAG GTCATCATCA CAAGCAAACT
1561TTATTAAAAA AAAAACTAGA AGTGTGCTTT TGCCAACTTT CTTGTACAAA GTGGTTGATA
1621TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1681CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCGGC
1741TGATGGCCGA TATAAGTCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1801tgaaagatt

Download FASTA (ORF) (Full)