Transcript: Human NM_001321502.2

Homo sapiens septin 10 (SEPTIN10), transcript variant 8, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
SEPTIN10 (151011)
Length:
4537
CDS:
140..1150

Additional Resources:

NCBI RefSeq record:
NM_001321502.2
NBCI Gene record:
SEPTIN10 (151011)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321502.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000144316 CAAAGCAGATACGGTTTCTAA pLKO.1 694 CDS 100% 5.625 7.875 N SEPTIN10 n/a
2 TRCN0000142317 GCCTTATTATGGCTGACGTAT pLKO.1 3913 3UTR 100% 4.950 6.930 N SEPTIN10 n/a
3 TRCN0000140647 GCTGGTGAACAGATCCATTCA pLKO.1 235 CDS 100% 4.950 3.960 N SEPTIN10 n/a
4 TRCN0000141285 CCAAAGCAGATACGGTTTCTA pLKO.1 693 CDS 100% 5.625 3.938 N SEPTIN10 n/a
5 TRCN0000140716 GAAGGACAAGGACCGTAAGAA pLKO.1 1266 3UTR 100% 5.625 3.938 N SEPTIN10 n/a
6 TRCN0000142403 GTCGGAAACAAGATGGTCAAA pLKO.1 878 CDS 100% 4.950 3.465 N SEPTIN10 n/a
7 TRCN0000139056 CAGGCCAAATTTGAGCACCTT pLKO.1 1102 CDS 100% 2.640 1.848 N SEPTIN10 n/a
8 TRCN0000142614 GAACCTTGACAGCAAGGTAAA pLKO.1 655 CDS 100% 1.080 0.756 N SEPTIN10 n/a
9 TRCN0000139746 CCCAACGGATGATGACACTAT pLKO.1 787 CDS 100% 4.950 2.970 N SEPTIN10 n/a
10 TRCN0000141055 CATGAGTGAATTGGTCAGCAA pLKO.1 745 CDS 100% 2.640 1.584 N SEPTIN10 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321502.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05045 pDONR223 100% 74% 71.1% None 30_31ins69;957_958ins133;1008_1009ins152 n/a
2 ccsbBroad304_05045 pLX_304 0% 74% 71.1% V5 30_31ins69;957_958ins133;1008_1009ins152 n/a
3 TRCN0000470390 ACGAGCTTACTATCCAGCAACTCG pLX_317 33.5% 74% 71.1% V5 30_31ins69;957_958ins133;1008_1009ins152 n/a
4 ccsbBroadEn_13268 pDONR223 100% 66.2% 63.7% None 30_31ins69;957_958ins133;1008_1009ins311 n/a
5 ccsbBroad304_13268 pLX_304 0% 66.2% 63.7% V5 30_31ins69;957_958ins133;1008_1009ins311 n/a
6 TRCN0000477479 GCGGCTGATGGCCGATATAAGTCT pLX_317 31.8% 66.2% 63.7% V5 30_31ins69;957_958ins133;1008_1009ins311 n/a
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