Construct: ORF TRCN0000477482
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011409.1_s317c1
- Derived from:
- ccsbBroadEn_01345
- DNA Barcode:
- ATTAACTGTCTATCAACCTAGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTPRO (5800)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477482
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5800 | PTPRO | protein tyrosine phosphatas... | NM_030667.3 | 100% | 100% | |
| 2 | human | 5800 | PTPRO | protein tyrosine phosphatas... | NM_002848.4 | 97.6% | 97.6% | 2625_2626ins84 |
| 3 | human | 5800 | PTPRO | protein tyrosine phosphatas... | XM_017019725.2 | 77.9% | 74.6% | (many diffs) |
| 4 | human | 5800 | PTPRO | protein tyrosine phosphatas... | XR_931316.3 | 52.9% | 1_393del;4042_6885del | |
| 5 | human | 5800 | PTPRO | protein tyrosine phosphatas... | NM_030669.3 | 33.3% | 33.3% | 0_1ins2433 |
| 6 | human | 5800 | PTPRO | protein tyrosine phosphatas... | NM_030671.3 | 33.3% | 33.3% | 0_1ins2433 |
| 7 | human | 5800 | PTPRO | protein tyrosine phosphatas... | NM_030668.3 | 31% | 31% | 0_1ins2433;192_193ins84 |
| 8 | human | 5800 | PTPRO | protein tyrosine phosphatas... | NM_030670.3 | 31% | 31% | 0_1ins2433;192_193ins84 |
| 9 | human | 5800 | PTPRO | protein tyrosine phosphatas... | XR_931318.2 | 24% | (many diffs) | |
| 10 | mouse | 19277 | Ptpro | protein tyrosine phosphatas... | NM_011216.3 | 84.6% | 90.9% | (many diffs) |
| 11 | mouse | 19277 | Ptpro | protein tyrosine phosphatas... | NM_001164401.1 | 82.5% | 88.8% | (many diffs) |
| 12 | mouse | 19277 | Ptpro | protein tyrosine phosphatas... | NM_001164402.1 | 29.4% | 31.7% | (many diffs) |
| 13 | mouse | 19277 | Ptpro | protein tyrosine phosphatas... | NM_001164403.1 | 27.3% | 29.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3717
- ORF length:
- 3648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggcacctg cccacgggga tacacggcgc ccgccgcctc ctgcctctgc 121 tctggctctt tgtgctgttc aagaatgcta cagctttcca tgtaactgtc caagatgata 181 ataacatcgt tgtctcatta gaagcttcag acgtcatcag tccagcatct gtgtatgttg 241 tgaagataac tggtgaatcc aaaaattatt tcttcgaatt tgaggaattc aacagcactt 301 tgcctcctcc tgttattttc aaggccagtt atcatggcct ttattatata atcactctgg 361 tagtggtaaa tggaaatgtg gtgaccaagc catccagatc aatcactgtg ttaacaaaac 421 ctctacctgt aaccagtgtt tccatatatg actataaacc ttctcctgaa acaggagtcc 481 tgtttgaaat acattatcca gaaaaatata acgttttcac aagagtgaac attagctact 541 gggaaggtaa agacttccgg acaatgctat ataaagattt ctttaaggga aaaacagtat 601 ttaatcactg gctgccagga atgtgttata gtaatatcac ctttcagctg gtatctgagg 661 caacttttaa taaaagtacc cttgttgagt acagtggtgt cagtcacgaa cccaaacagc 721 acagaactgc cccttatcca cctcaaaata tttccgttcg tatcgtaaac ttgaacaaaa 781 acaactggga agaacagagt ggcaatttcc cagaagaatc cttcatgaga tcacaagata 841 caataggaaa agaaaaactc ttccatttta cagaagaaac ccctgaaatt ccctcgggca 901 acatttcttc cggttggcct gattttaata gcagtgacta tgaaactacg tctcagccat 961 attggtggga cagtgcatct gcagctcctg aaagtgaaga tgaatttgtc agcgtacttc 1021 ccatggaata cgaaaataac agtacactca gtgagacaga gaagtcaaca tcaggctctt 1081 tctccttttt ccctgtgcaa atgatattga cctggttacc acccaaacca cccactgctt 1141 ttgatgggtt ccatatccat attgaacgag aagagaactt tactgaatat ttgatggtgg 1201 atgaagaagc acatgaattt gttgcagaac tgaaggaacc tgggaaatat aagttatctg 1261 tgacaacctt tagttcctca ggatcttgtg aaactcgaaa aagtcagtca gcaaaatcac 1321 tcagctttta tatcagtcct tcaggagagt ggattgaaga actgaccgag aagccgcagc 1381 acgtgagtgt ccacgtttta agctcaacca ctgccttgat gtcctggaca tcttcccaag 1441 agaactacaa cagcaccatt gtgtctgtgg tgtcgctgac ctgccagaaa caaaaggaga 1501 gccagaggct tgaaaagcag tactgcactc aggtgaactc aagcaaacct attattgaaa 1561 atctggttcc tggtgcccag taccaggttg taatatacct aaggaaaggc cctttgattg 1621 gaccaccttc agatcctgtg acatttgcta ttgttcccac aggaataaag gatttaatgc 1681 tctatccttt gggtcctacg gccgtggttc tgagctggac cagaccttat ttaggcgtgt 1741 tcagaaaata cgtggttgaa atgttttatt tcaaccctgc tacaatgaca tcagagtgga 1801 ccacctacta tgaaatagca gcaactgttt ccttaactgc atccgtgaga atagctaatc 1861 tgctgccagc atggtactac aacttccggg ttaccatggt gacgtgggga gatccagaat 1921 tgagctgctg tgacagctct accatcagct tcataacagc cccagtggct ccggaaatca 1981 cttctgtgga atatttcaac agtctgttat atatcagttg gacatatggg gatgatacaa 2041 cggacttgtc ccattctaga atgcttcact ggatggtggt tgcagaagga aaaaagaaaa 2101 ttaaaaagag tgtaacacgc aatgtcatga ctgcaattct cagcttgcct ccaggcgaca 2161 tctataacct ctcagtaact gcttgtactg aaagaggaag taatacctcc atgctccgcc 2221 ttgtcaagct agaaccagct ccacccaaat cactcttcgc agtgaacaaa acccagactt 2281 cagtgacttt gctgtgggtg gaagagggag tagctgattt ctttgaagtt ttctgtcaac 2341 aagttggctc cagtcagaaa accaaacttc aggaaccagt tgctgtttct tcccatgtcg 2401 tgaccatctc cagccttctt cctgccactg cctacaattg tagtgtcacc agctttagcc 2461 atgacagccc cagtgtccct acgttcatag ccgtctcaac aatggttaca gagatgaatc 2521 ccaatgtggt agtgatctcc gtgctggcca tccttagcac acttttaatt ggactgttgc 2581 ttgttaccct cattattctt aggaaaaagc atctgcagat ggctagggag tgtggagctg 2641 gtacatttgt caattttgca tccttagaga gggatggaaa gcttccatac aactggcgta 2701 ggagtatatt tgctttctta accctgctac cctcatgtct ttggactgat tatcttttgg 2761 cattttatat taatccttgg agtaaaaatg gtttaaagaa gaggaaactg acaaacccgg 2821 ttcaactgga tgactttgat gcctatatta aggatatggc caaagactct gactataaat 2881 tttctcttca gtttgaggag ttgaaattga ttggactgga tatcccacac tttgctgcag 2941 atcttccact gaatcgatgt aaaaaccgtt acacaaacat cctaccatat gacttcagcc 3001 gtgtgagatt agtctccatg aatgaagagg aaggtgcaga ctacatcaat gccaactata 3061 ttcctggata caactcaccc caggagtata ttgccaccca ggggccactg cctgaaacca 3121 gaaatgactt ctggaagatg gtcctgcaac aaaagtctca gattattgtc atgctcactc 3181 agtgtaatga gaaaaggagg gtgaaatgtg accattactg gccattcacg gaagaaccta 3241 tagcctatGG AGACATCACT GTGGAGATGA TTTCAGAGGA AGAGCAGGAC GACTGGGCCT 3301 GTAGACACTT CCGGATCAAC TATGCTGACG AGATGCAGGA TGTGATGCAT TTTAACTACA 3361 CTGCATGGCC TGATCATGGT GTGCCCACAG CAAATGCTGC AGAAAGTATC CTGCAGTTTG 3421 TACACATGGT CCGACAGCAA GCTACCAAGA GCAAAGGTCC CATGATCATT CACTGCAGTG 3481 CTGGCGTGGG ACGGACAGGA ACATTCATTG CCCTGGACAG GCTCTTGCAG CACATTCGGG 3541 ATCATGAGTT TGTTGACATC TTAGGGCTGG TGTCAGAAAT GAGGTCATAC CGGATGTCTA 3601 TGGTACAGAC AGAGGAGCAG TACATTTTTA TCCATCAGTG TGTGCAACTG ATGTGGATGA 3661 AGAAGAAGCA GCAGTTCTGC ATCAGTGATG TCATATACGA GAATGTTAGC AAGTCCTTGC 3721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3841 ATATATCTTG TGGAAAGGAC GAATTAACTG TCTATCAACC TAGATCACGC GTTAAGTCga 3901 caatcaacct ctggattaca aaatttgtga aagatt