Construct: ORF TRCN0000477482

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011409.1_s317c1
Derived from:
ccsbBroadEn_01345
DNA Barcode:
ATTAACTGTCTATCAACCTAGATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PTPRO (5800)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477482
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5800 PTPRO protein tyrosine phosphatas... NM_030667.3 100% 100%
2 human 5800 PTPRO protein tyrosine phosphatas... NM_002848.4 97.6% 97.6% 2625_2626ins84
3 human 5800 PTPRO protein tyrosine phosphatas... XM_017019725.2 77.9% 74.6% (many diffs)
4 human 5800 PTPRO protein tyrosine phosphatas... XR_931316.3 52.9% 1_393del;4042_6885del
5 human 5800 PTPRO protein tyrosine phosphatas... NM_030669.3 33.3% 33.3% 0_1ins2433
6 human 5800 PTPRO protein tyrosine phosphatas... NM_030671.3 33.3% 33.3% 0_1ins2433
7 human 5800 PTPRO protein tyrosine phosphatas... NM_030668.3 31% 31% 0_1ins2433;192_193ins84
8 human 5800 PTPRO protein tyrosine phosphatas... NM_030670.3 31% 31% 0_1ins2433;192_193ins84
9 human 5800 PTPRO protein tyrosine phosphatas... XR_931318.2 24% (many diffs)
10 mouse 19277 Ptpro protein tyrosine phosphatas... NM_011216.3 84.6% 90.9% (many diffs)
11 mouse 19277 Ptpro protein tyrosine phosphatas... NM_001164401.1 82.5% 88.8% (many diffs)
12 mouse 19277 Ptpro protein tyrosine phosphatas... NM_001164402.1 29.4% 31.7% (many diffs)
13 mouse 19277 Ptpro protein tyrosine phosphatas... NM_001164403.1 27.3% 29.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3717
ORF length:
3648
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggggcacctg cccacgggga tacacggcgc ccgccgcctc ctgcctctgc
121tctggctctt tgtgctgttc aagaatgcta cagctttcca tgtaactgtc caagatgata
181ataacatcgt tgtctcatta gaagcttcag acgtcatcag tccagcatct gtgtatgttg
241tgaagataac tggtgaatcc aaaaattatt tcttcgaatt tgaggaattc aacagcactt
301tgcctcctcc tgttattttc aaggccagtt atcatggcct ttattatata atcactctgg
361tagtggtaaa tggaaatgtg gtgaccaagc catccagatc aatcactgtg ttaacaaaac
421ctctacctgt aaccagtgtt tccatatatg actataaacc ttctcctgaa acaggagtcc
481tgtttgaaat acattatcca gaaaaatata acgttttcac aagagtgaac attagctact
541gggaaggtaa agacttccgg acaatgctat ataaagattt ctttaaggga aaaacagtat
601ttaatcactg gctgccagga atgtgttata gtaatatcac ctttcagctg gtatctgagg
661caacttttaa taaaagtacc cttgttgagt acagtggtgt cagtcacgaa cccaaacagc
721acagaactgc cccttatcca cctcaaaata tttccgttcg tatcgtaaac ttgaacaaaa
781acaactggga agaacagagt ggcaatttcc cagaagaatc cttcatgaga tcacaagata
841caataggaaa agaaaaactc ttccatttta cagaagaaac ccctgaaatt ccctcgggca
901acatttcttc cggttggcct gattttaata gcagtgacta tgaaactacg tctcagccat
961attggtggga cagtgcatct gcagctcctg aaagtgaaga tgaatttgtc agcgtacttc
1021ccatggaata cgaaaataac agtacactca gtgagacaga gaagtcaaca tcaggctctt
1081tctccttttt ccctgtgcaa atgatattga cctggttacc acccaaacca cccactgctt
1141ttgatgggtt ccatatccat attgaacgag aagagaactt tactgaatat ttgatggtgg
1201atgaagaagc acatgaattt gttgcagaac tgaaggaacc tgggaaatat aagttatctg
1261tgacaacctt tagttcctca ggatcttgtg aaactcgaaa aagtcagtca gcaaaatcac
1321tcagctttta tatcagtcct tcaggagagt ggattgaaga actgaccgag aagccgcagc
1381acgtgagtgt ccacgtttta agctcaacca ctgccttgat gtcctggaca tcttcccaag
1441agaactacaa cagcaccatt gtgtctgtgg tgtcgctgac ctgccagaaa caaaaggaga
1501gccagaggct tgaaaagcag tactgcactc aggtgaactc aagcaaacct attattgaaa
1561atctggttcc tggtgcccag taccaggttg taatatacct aaggaaaggc cctttgattg
1621gaccaccttc agatcctgtg acatttgcta ttgttcccac aggaataaag gatttaatgc
1681tctatccttt gggtcctacg gccgtggttc tgagctggac cagaccttat ttaggcgtgt
1741tcagaaaata cgtggttgaa atgttttatt tcaaccctgc tacaatgaca tcagagtgga
1801ccacctacta tgaaatagca gcaactgttt ccttaactgc atccgtgaga atagctaatc
1861tgctgccagc atggtactac aacttccggg ttaccatggt gacgtgggga gatccagaat
1921tgagctgctg tgacagctct accatcagct tcataacagc cccagtggct ccggaaatca
1981cttctgtgga atatttcaac agtctgttat atatcagttg gacatatggg gatgatacaa
2041cggacttgtc ccattctaga atgcttcact ggatggtggt tgcagaagga aaaaagaaaa
2101ttaaaaagag tgtaacacgc aatgtcatga ctgcaattct cagcttgcct ccaggcgaca
2161tctataacct ctcagtaact gcttgtactg aaagaggaag taatacctcc atgctccgcc
2221ttgtcaagct agaaccagct ccacccaaat cactcttcgc agtgaacaaa acccagactt
2281cagtgacttt gctgtgggtg gaagagggag tagctgattt ctttgaagtt ttctgtcaac
2341aagttggctc cagtcagaaa accaaacttc aggaaccagt tgctgtttct tcccatgtcg
2401tgaccatctc cagccttctt cctgccactg cctacaattg tagtgtcacc agctttagcc
2461atgacagccc cagtgtccct acgttcatag ccgtctcaac aatggttaca gagatgaatc
2521ccaatgtggt agtgatctcc gtgctggcca tccttagcac acttttaatt ggactgttgc
2581ttgttaccct cattattctt aggaaaaagc atctgcagat ggctagggag tgtggagctg
2641gtacatttgt caattttgca tccttagaga gggatggaaa gcttccatac aactggcgta
2701ggagtatatt tgctttctta accctgctac cctcatgtct ttggactgat tatcttttgg
2761cattttatat taatccttgg agtaaaaatg gtttaaagaa gaggaaactg acaaacccgg
2821ttcaactgga tgactttgat gcctatatta aggatatggc caaagactct gactataaat
2881tttctcttca gtttgaggag ttgaaattga ttggactgga tatcccacac tttgctgcag
2941atcttccact gaatcgatgt aaaaaccgtt acacaaacat cctaccatat gacttcagcc
3001gtgtgagatt agtctccatg aatgaagagg aaggtgcaga ctacatcaat gccaactata
3061ttcctggata caactcaccc caggagtata ttgccaccca ggggccactg cctgaaacca
3121gaaatgactt ctggaagatg gtcctgcaac aaaagtctca gattattgtc atgctcactc
3181agtgtaatga gaaaaggagg gtgaaatgtg accattactg gccattcacg gaagaaccta
3241tagcctatGG AGACATCACT GTGGAGATGA TTTCAGAGGA AGAGCAGGAC GACTGGGCCT
3301GTAGACACTT CCGGATCAAC TATGCTGACG AGATGCAGGA TGTGATGCAT TTTAACTACA
3361CTGCATGGCC TGATCATGGT GTGCCCACAG CAAATGCTGC AGAAAGTATC CTGCAGTTTG
3421TACACATGGT CCGACAGCAA GCTACCAAGA GCAAAGGTCC CATGATCATT CACTGCAGTG
3481CTGGCGTGGG ACGGACAGGA ACATTCATTG CCCTGGACAG GCTCTTGCAG CACATTCGGG
3541ATCATGAGTT TGTTGACATC TTAGGGCTGG TGTCAGAAAT GAGGTCATAC CGGATGTCTA
3601TGGTACAGAC AGAGGAGCAG TACATTTTTA TCCATCAGTG TGTGCAACTG ATGTGGATGA
3661AGAAGAAGCA GCAGTTCTGC ATCAGTGATG TCATATACGA GAATGTTAGC AAGTCCTTGC
3721CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
3781TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
3841ATATATCTTG TGGAAAGGAC GAATTAACTG TCTATCAACC TAGATCACGC GTTAAGTCga
3901caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)